Comparative Analysis of Fluorescence Reporter Signals Based on Intensity, Anisotropy, Time-Resolution, and Wavelength-Ratiometry

Demchenko, Alexander P.

doi:10.1007/978-3-642-04702-2_1

Cited by 16 publications

(7 citation statements)

References 62 publications

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“…Such a red shift in emission has also been reported for PMMA films doped with Rh6G . The observed red shift can be indicative of dye–dye interactions between the fluorophore molecules in different environments, for example, Förster resonance energy transfer (homo-FRET, i.e., Förster resonance energy transfer between chemically identical molecules). − This, however, is not a change of the individual spectra of the respective dye molecules but an apparent red shift of the overall spectrum of each stained bead . As underpinned by the spectroscopic properties of differently sized beads loaded with varying amounts of Rh6G, the red shift in emission is characteristic of small beads, whereas the occurrence and size of spectral broadening is governed by both the loading concentration and the bead size.…”

Section: Results and Discussionmentioning

confidence: 99%

Close Spectroscopic Look at Dye-Stained Polymer Microbeads

et al. 2018

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Dye-stained micrometer-sized polymer beads are important tools in the life sciences with applications in biomedical, biochemical, and clinical research. Here, bead-based assays are increasingly used, for example, in DNA sequencing and the detection of autoimmune diseases or pathogenic microorganisms. Moreover, stained beads are employed as calibration tools for fluorescence microscopy and flow cytometry methods with increasing complexity. To address the requirements concerning the relevant fluorescence features, the spectroscopic properties of representative polymer beads with diameters ranging from about 1 to 10 μm stained with varying concentrations of rhodamine 6G were systematically assessed. The observed dependence of the spectral properties, fluorescence decay kinetics, and fluorescence quantum yields on bead size and dye loading concentration is attributed to different fluorescence characteristics of fluorophores located in the particle core and near-surface dye molecules. Supported by the fluorescence anisotropy measurements, the origin of the observed alteration of fluorescence features is ascribed to a combination of excitation energy transfer and polarity-related effects that are especially pronounced at the interface of the bead and the surrounding medium. The results of our studies underline the need to carefully control and optimize all parameters that can affect the fluorescence properties of the dye-stained beads.

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Section: Results and Discussionmentioning

confidence: 99%

Close Spectroscopic Look at Dye-Stained Polymer Microbeads

et al. 2018

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“…It will identify the noise of pest wingbeats or sound by their mouth of anything else for their basic instinct [15].  Thermal sensor: This sensor is used to sensing fluorescence [16]. Chlorophyll fluorescence will be identified by this sensor as a healthy leaf's fluorescence data will be stored previously.…”

Section: System Architecturementioning

confidence: 99%

IFSG: Intelligence agriculture crop-pest detection system using IoT automation system

Salehin

Noman

Ul-Islam

et al. 2021

IJEECS

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The agricultural and technological combination is blessed for modern world life. Internet of things (IoT) is essential for comfort and development to our agriculture side. In our study, we detected the various pest using different types of sensors and this information has automatically sent to the farmer's mobile for the alert. All these sensors had a central database. Those sensors collect all the data and display the results compared to the central data. The High-image sensor will be able to detect all the rays emitted from the plant and another one is the gas sensor which is able to detect all the gases coming from the diseased plant. We mainly use sound sensor, MQ138, CMOSOV-7670, AMG-8833 for a better automation system. We test it with real-time environment conditions (40°C≤TA≤14°C). Crop pest detection automatic process is more efficient than the other detection process according to testing output. As a result, far-reaching changes in the agricultural sector are possible. To reduce extra cost and increasing more farming ability we need to IoT and Agriculture combinations more.

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“…Long-wavelength organic fluorophores are expected to have shorter fluorescence lifetimes as compared to those emitting in the UV/visible. The lifetime properties are in particular important when it comes to measuring fluorescence polarization as the molecular weight of the antigen that can be measured in an assay is directly related to the lifetime of the fluorescently labeled tracer molecule [2]. Again, new developments in this field are showing promising results also in this direction and are discussed below.…”

Section: Introductionmentioning

confidence: 97%

“…FLT has several advantages as compared to conventional methods. In particular in high throughput screening (HTS), where the assay robustness is of utmost importance, FLT promises great potential to reduce the number of undesired false positives and false negatives results [1,2]. Despite its advantages, fluorescence lifetime is a parameter that has only been moderately explored for use in biomedical assays.…”

mentioning

confidence: 99%

Long-Wavelength Probes and Labels Based on Cyanines and Squaraines

Patsenker

Tatarets

Terpetschnig³

2010

Springer Series on Fluorescence

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In this review, we make an attempt to compare the characteristics and applications of red and near infrared cyanine and squaraine dyes used for biological research, biomedical assays, and high-throughput screening. While the favorable photophysical properties of cyanine dyes makes them predestined as covalent labels, the environmentally sensitive squaraine dyes are utilizable as both florescent probes and labels. Reducing the aggregation tendencies of these dyes in aqueous media seems to be one of the most promising ways to improve their brightness, fluorescence lifetimes, and photostability. Indolenine-based squaraines including ring-substituted squaraines exhibit great potential for the design of bright and sensitive fluorescent probes and labels with increased photostability.

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Comparative Analysis of Fluorescence Reporter Signals Based on Intensity, Anisotropy, Time-Resolution, and Wavelength-Ratiometry

Cited by 16 publications

References 62 publications

Close Spectroscopic Look at Dye-Stained Polymer Microbeads

Close Spectroscopic Look at Dye-Stained Polymer Microbeads

IFSG: Intelligence agriculture crop-pest detection system using IoT automation system

Long-Wavelength Probes and Labels Based on Cyanines and Squaraines

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