Comparative analysis of genotypic diversity in Entamoeba nuttalli isolates from Tibetan macaques and rhesus macaques in China

Guan, Yue; Feng, Min; Cai, Junlong; Min, Xiangyang; Zhou, Xingyu; Xu, Qing; Tan, Ning; Cheng, Xunjia; Tachibana, Hiroshi

doi:10.1016/j.meegid.2015.12.014

Cited by 21 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are numerous wild macaque populations in China, in which the rhesus macaque is widely distributed, but the Tibetan macaque is unique to east central China. Our recent studies have detected E. nuttalli infection in wild rhesus and Tibetan macaques in China (1,8), indicating that these macaques are also natural hosts of E. nuttalli.…”

Section: Introductionmentioning

confidence: 84%

“…It has been demonstrated that genetic diversity of Chinese E. nuttalli isolates is associated with geographic distance based on the tRNA-STR loci (8). The importance of the geographic factor was proved by investigation of other E. nuttalli isolates in Nepal (16).…”

Section: Discussionmentioning

confidence: 99%

“…Stool samples, PCR-positive for E. nuttalli, were xenically cultured in modified Tanabe-Chiba medium (17). In addition, trophozoites of two E. nuttalli isolates from Mount Qian-ling (GY) of the Guizhou Province and six E. nuttalli isolates from Mount E-mei (EM) of the Sichuan Province were axenically cultured in YIMDHA-S medium (1,8). Trophozoites from 16 E. nuttalli isolates from wild rhesus macaques in Mount Long-hu (LH) of the Guanxi Province were xenically cultured in modified Tanabe-Chiba medium.…”

Section: Stool Examination and Culturementioning

confidence: 99%

“…tRNA-linked short tandem repeats (STRs) were another effective polymorphic marker (16). Recent studies on E. nuttalli isolate genotypes have suggested that these are correlated with the geographical distribution of host macaques (8,16).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Correlation of genetic diversity between hosts and parasites in <i>Entamoeba nuttalli</i> isolates from Tibetan and rhesus macaques in China

Miao

Feng

Guan

et al. 2018

BST

Self Cite

View full text Add to dashboard Cite

Entamoeba nuttalli infection is prevalent in captive and wild macaques. Recent studies have suggested that genotypes of E. nuttalli isolates are correlated with the geographical distribution of host macaques. Correlation of amoebic genotypes with genetic diversity of host macaques was analyzed in present study. Sixty fresh stool samples were obtained from wild Tibetan macaques living in Mount Huang (HS) of the An-hui Province in China. PCR analysis revealed that the most prevalent Entamoeba species was E. chattoni (E. polecki ST2) (86.7%) followed by E. nuttalli (58.3%) and E. coli (25%). Six E. nuttalli HS isolates were successfully cultured. The tRNA-linked short tandem repeat (STR) loci and serine-rich protein gene of E. nuttalli isolates from four different regions of China (Mount Long-hu, Gui-yang, Mount E-mei, and HS, the former three isolates were obtained in previous studies) were studied and high numbers of polymorphisms were detected. When genetic diversity of different populations of E. nuttalli isolates was compared with geographical distance, an r value of 0.919 was assigned by a Mantel test based on the tRNA-STR loci. In host macaques, the mtDNA HVS-I gene was also highly polymorphic in each of the genomes. Multiple regression analysis using E. nuttalli tRNA-STR loci genetic, macaque mtDNA HVS-I gene, and geographic distances showed an r value of 0.943, indicating that a higher relevance was demonstrated when geographic and host gene factors were considered. Analysis of genetic factor of host would benefit for better understanding of the evolution of E. nuttalli.

show abstract

Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Stool Examination and Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Correlation of genetic diversity between hosts and parasites in <i>Entamoeba nuttalli</i> isolates from Tibetan and rhesus macaques in China

Miao

Feng

Guan

et al. 2018

BST

Self Cite

View full text Add to dashboard Cite

show abstract

“…In cases of coinfections by diverse clones or ameba species (as reported in human, great ape, and rhesus‐macaque hosts; Guan et al. ; Ngobeni et al. ; Vlčková et al.…”

Section: Implications Of Clone Discrimination Ability For E Histolytmentioning

confidence: 99%

Discrimination Experiments in Entamoeba and Evidence from Other Protists Suggest Pathogenic Amebas Cooperate with Kin to Colonize Hosts and Deter Rivals

Espinosa

Paz-y-Miño-C

2018

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Entamoeba histolytica is one of the least understood protists in terms of taxa, clone, and kin discrimination/recognition ability. However, the capacity to tell apart same or self (clone/kin) from different or nonself (nonclone/nonkin) has long been demonstrated in pathogenic eukaryotes like Trypanosoma and Plasmodium, free-living social amebas (Dictyostelium, Polysphondylium), budding yeast (Saccharomyces), and in numerous bacteria and archaea (prokaryotes). Kin discrimination/recognition is explained under inclusive fitness theory; that is, the reproductive advantage that genetically closely related organisms (kin) can gain by cooperating preferably with one another (rather than with distantly related or unrelated individuals), minimizing antagonism and competition with kin, and excluding genetic strangers (or cheaters = noncooperators that benefit from others' investments in altruistic cooperation). In this review, we rely on the outcomes of in vitro pairwise discrimination/recognition encounters between seven Entamoeba lineages to discuss the biological significance of taxa, clone, and kin discrimination/recognition in a range of generalist and specialist species (close or distantly related phylogenetically). We then focus our discussion on the importance of these laboratory observations for E. histolytica's life cycle, host infestation, and implications of these features of the amebas' natural history for human health (including mitigation of amebiasis).

show abstract

First Detection and Molecular Identification of Entamoeba in Yaks from China

et al. 2020

View full text Add to dashboard Cite

Background Yak, a predominant livestock of plateau areas, is known as a host to many parasites. And the genus Entamoeba, the third-common cause of the mortality worldwide from parasitic diseases, was discovered in yaks once. Methods We investigated the distribution and species of Entamoeba spp. from yaks in Qinghai province, northwestern China, by collecting 1027 yak fecal samples. All samples were divided according to seven geographical sites, four seasons, and two age groups of yaks. After extracting DNA, polymerase chain reaction (PCR) was performed to amplify the 18S rRNA gene, and sequences were analyzed with phylogenetic method. ResultsWe observed an overall Entamoeba positive rate of 36.32% (373/1027) in yaks from Qinghai province. The common species included Entamoeba bovis (284/373), Entamoeba sp. MG107/BEL (79/373), Entamoeba sp. ribosomal lineage (RL) two (8/373), and Entamoeba sp. RL9 (2/373). According to the result of statistical analysis, Entamoeba infection rate was the highest in summer and significantly differed from that observed during other seasons (P < 0.05). The yaks from Golog had the highest prevalence of Entamoeba among all geographical origins in Qinghai province (P < 0.05). However, no significant difference was observed (P > 0.05) among different age groups, as evident from a positive rate of 39.58% in ≤ 6-month and 36.16% in > 6-month yaks. Conclusion These results indicate the prevalence and predominant species of Entamoeba in yaks. To our knowledge, this is the first study to report E. bovis, Entamoeba sp. RL2, and Entamoeba sp. RL9 in Chinese yaks. Keywords Qinghai • Yak • Entamoeba • 18S rRNA • Phylogeny Abbreviations PCR Polymerase chain reaction RL Ribosomal lineages χ 2 test Chi-square test/Chi-square goodness-of-fit test BLAST Basic local alignment search tool * Qing Lin

show abstract

Comparative analysis of genotypic diversity in Entamoeba nuttalli isolates from Tibetan macaques and rhesus macaques in China

Cited by 21 publications

References 30 publications

Correlation of genetic diversity between hosts and parasites in <i>Entamoeba nuttalli</i> isolates from Tibetan and rhesus macaques in China

Correlation of genetic diversity between hosts and parasites in <i>Entamoeba nuttalli</i> isolates from Tibetan and rhesus macaques in China

Discrimination Experiments in Entamoeba and Evidence from Other Protists Suggest Pathogenic Amebas Cooperate with Kin to Colonize Hosts and Deter Rivals

First Detection and Molecular Identification of Entamoeba in Yaks from China

Contact Info

Product

Resources

About