Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

Pérez‐Hedo, Meritxell; Sánchez-López, Isabel; Eizaguirre, Matilde

doi:10.1186/1477-5956-10-58

Cited by 10 publications

(8 citation statements)

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“…The large majority of these previously described proteins are very abundant hemolymph components as inferred from spectral counting [48] (Table S2). Similarly, previous analyses of the hemolymph proteome in other insects (including the bee Apis mellifera , the silkworm Bombyx mori , and the tobacco hornworm Manduca sexta ) have been of comparatively limited scope, revealing primarily abundant constituents [10], [15], [19], [24], [49], [50], [51], [52], [53], [54].…”

Section: Resultsmentioning

confidence: 99%

The Hemolymph Proteome of Fed and Starved Drosophila Larvae

et al. 2013

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The co-operation of specialized organ systems in complex multicellular organisms depends on effective chemical communication. Thus, body fluids (like blood, lymph or intraspinal fluid) contain myriads of signaling mediators apart from metabolites. Moreover, these fluids are also of crucial importance for immune and wound responses. Compositional analyses of human body fluids are therefore of paramount diagnostic importance. Further improving their comprehensiveness should increase our understanding of inter-organ communication. In arthropods, which have trachea for gas exchange and an open circulatory system, the single dominating interstitial fluid is the hemolymph. Accordingly, a detailed analysis of hemolymph composition should provide an especially comprehensive picture of chemical communication and defense in animals. Therefore we used an extensive protein fractionation workflow in combination with a discovery-driven proteomic approach to map out the detectable protein composition of hemolymph isolated from Drosophila larvae. Combined mass spectrometric analysis revealed more than 700 proteins extending far beyond the previously known Drosophila hemolymph proteome. Moreover, by comparing hemolymph isolated from either fed or starved larvae, we provide initial provisional insights concerning compositional changes in response to nutritional state. Storage proteins in particular were observed to be strongly reduced by starvation. Our hemolymph proteome catalog provides a rich basis for data mining, as exemplified by our identification of potential novel cytokines, as well as for future quantitative analyses by targeted proteomics.

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Section: Resultsmentioning

confidence: 99%

The Hemolymph Proteome of Fed and Starved Drosophila Larvae

et al. 2013

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“…The haemolymph also contains proteins with roles in immunity [50–53], development [54–56], olfaction [48, 57] and neurotransmission [58, 59]. Profiling changes in haemolymph protein abundance has the potential to resolve global molecular signatures and biomarkers underlying key life stage events [59–61], including diapause [62]. Here, using mass spectrometry-based proteomics, we aim to establish a clear understanding of the molecular mechanisms underlying the ecophysiological stages of onset, maintenance and termination of diapause, by profiling the haemolymph proteome throughout diapause for the buff-tailed bumblebee, B. terrestris .…”

Section: Introductionmentioning

confidence: 99%

Mating precedes selective immune priming which is maintained throughout bumblebee queen diapause

et al. 2019

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BackgroundUnderstanding the mechanisms by which organisms adapt to unfavourable conditions is a fundamental question in ecology and evolutionary biology. One such mechanism is diapause, a period of dormancy typically found in nematodes, fish, crustaceans and insects. This state is a key life-history event characterised by arrested development, suppressed metabolism and increased stress tolerance and allows an organism to avoid prolonged periods of harsh and inhospitable environmental conditions. For some species, diapause is preceded by mating which can have a profound effect on female behaviour, physiology and key biological processes, including immunity. However, our understanding of how mating impacts long-term immunity and whether these effects persist throughout diapause is currently limited. To address this, we explored molecular changes in the haemolymph of the ecologically important pollinator, the buff-tailed bumblebee Bombus terrestris. B. terrestris queens mate prior to entering diapause, a non-feeding period of arrested development that can last 6–9 months. Using mass-spectrometry-based proteomics, we quantified changes in the pre-diapause queen haemolymph after mating, as well as the subsequent protein expression of mated queens during and post-diapause.ResultsOur analysis identified distinct proteome profiles associated with diapause preparation, maintenance and termination. More specifically, mating pre-diapause was followed by an increase in the abundance of antimicrobial peptides, key effectors of the immune system. Furthermore, we identified the elevated abundance of these proteins to be maintained throughout diapause. This finding was in contrast to the general reduction observed in immune proteins during diapause suggestive of selective immune priming and expression during diapause. Diapause also affected the expression of proteins involved in cuticular maintenance, olfaction, as well as proteins of unknown function, which may have roles in diapause regulation.ConclusionsOur results provide clear molecular evidence for the consequences and benefits of mating at the immune level as it precedes the selective increased abundance of antimicrobial peptides that are sustained throughout diapause. In addition, our results provide novel insights into the molecular mechanisms by which bumblebees prepare for, survive, and recover from diapause, insights that may have implications for our general understanding of these processes in other insect groups.

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“…The haemolymph also contains proteins with roles in immunity [50][51][52][53], development [54][55][56], olfaction [48,57] and neurotransmission [58,59]. Profiling changes in haemolymph protein abundance has the potential to resolve global molecular signatures and biomarkers underlying key life stage events [59][60][61], including diapause [62]. Here, using mass spectrometry-based proteomics, we aim to establish a clear understanding of the molecular mechanisms underlying the ecophysiological stages of onset, maintenance and termination of diapause, by profiling the haemolymph proteome throughout diapause for the buff-tailed bumblebee,…”

Section: Introductionmentioning

confidence: 99%

Mating precedes selective immune priming which is maintained throughout bumblebee queen diapause

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et al. 2019

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Background: Understanding the mechanisms by which organisms adapt to unfavourable conditions is a fundamental question in ecology and evolutionary biology. One such mechanism is diapause, a period of dormancy typically found in nematodes, fish, crustaceans and insects. This state is a key life-history event characterised by arrested development, suppressed metabolism and increased stress tolerance and allows an organism to avoid prolonged periods of harsh and inhospitable environmental conditions. For some species, diapause is preceded by mating which can have a profound effect on female behaviour, physiology and key biological processes, including immunity. However, our understanding of how mating impacts long-term immunity and whether these effects persist throughout diapause is currently limited. To address this, we explored molecular changes in the haemolymph of the ecologically important pollinator, the buff-tailed bumblebee Bombus terrestris . B. terrestris queens mate prior to entering diapause, a non-feeding period of arrested development that can last 6-9 months. Using mass-spectrometry-based proteomics, we quantified changes in the pre-diapause queen haemolymph after mating, as well as the subsequent protein expression of mated queens during and post-diapause. Results: Our analysis identified distinct proteome profiles associated with diapause preparation, maintenance and termination. More specifically, mating pre-diapause was followed by an increase in the abundance of antimicrobial peptides, key effectors of the immune system. Furthermore, we identified the elevated abundance of these proteins to be maintained throughout diapause. This finding was in contrast to a general reduction in immune proteins during diapause suggestive of selective immune priming and expression during diapause. Diapause also affected the expression of proteins involved in cuticular maintenance, olfaction, as well as proteins of unknown function, which may have roles in diapause regulation. Conclusions: Our results provide clear molecular evidence for the consequences and benefits of mating at the immune level as it precedes the selective increased abundance of antimicrobial peptides that are sustained throughout diapause. In addition, our results provide novel insights into the molecular mechanisms by which bumblebees prepare for, survive, and recover from diapause, insights that may have implications for our general understanding of these processes in other insect groups.

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Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

Cited by 10 publications

References 47 publications

The Hemolymph Proteome of Fed and Starved Drosophila Larvae

The Hemolymph Proteome of Fed and Starved Drosophila Larvae

Mating precedes selective immune priming which is maintained throughout bumblebee queen diapause

Mating precedes selective immune priming which is maintained throughout bumblebee queen diapause

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