Comparative analysis of mesenchymal stem cells cultivated in serum free media

Abstract

Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity.

Comprehensive assessment of goat adipose tissue-derived mesenchymal stem cells cultured in different media

“…CFSE is an effective and widely employed method for monitoring the division of lymphocytes 71 , 72 . Additionally, it is a valuable tool for assessing the proliferation of MSCs 73 – 75 . CFSE covalently attaches to enduring intracellular molecules, imparting them with the fluorescent dye carboxyfluorescein.…”

Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiation

“…This increased DNA concentration in ACF and XF media formulations can be attributed to the various additives used to replace animal sera. For example, a previous study showed that adipose-derived mesenchymal stromal cells cultured in serum-free media had a higher population doubling time compared to adipose-derived mesenchymal stromal cells cultured with FBS ( Lee et al, 2022 ). Another study showed that hBMSCs cultured in serum-free media had higher proliferation rates compared to cells cultured in serum-containing media ( Chase et al, 2010 ).…”

Recent advances in extracellular vesicle engineering and its applications to regenerative medicine

“… Product name Company Country Ref. 1 MSC NutriStem® XF Sartorius Germany [ 28 ] 2 CellCor™ CD MSC Xcell Therapeutics Korea [ 25 ] 3 KBM ADSC4 KOHJIN BIO Japan [ 29 ] 4 MSC Xeno-Free Culture Medium Cellartis Japan [ 30 ] 5 StemXVivo Xeno-Free Human MSC Expansion Media R&D Systems™ USA [ 31 ] 6 RoosterNourish™-MSC-XF RoosterBio USA [ 32 ] 7 StemPro MSC SFM XenoFree Gibco USA [ 33 ] …”