Comparative analysis of microbial community in the whole body and midgut from fully engorged and unfed female adult Melophagus ovinus

Abstract

Melophagus ovinus is a type of ectoparasite infesting sheep. Data regarding the comprehensive bacterial community associated with the whole body and midgut of M. ovinus under different engorged statuses are required. Melophagus ovinus were collected from the city of Jiuquan, China. Bacterial DNA was extracted from the whole body and midgut of fully engorged female adults, or newly hatched and unfed adult female M. ovinus. The 16S rRNA gene V3-V4 hypervariable regions were sequenced using the IonS5™XL platform (Thermo Fisher Scientific, Waltham, MA, U.S.A.). The whole body bacterial diversity of the newly hatched, unfed adult females was greater compared with that of the other three samples. Proteobacteria was the dominant bacterial phylum in all of the samples. Of the 42 total bacterial genera present in all of the experimental samples, Arsenophonus, Bartonella and Wolbachia were the dominant genera. The relative abundance of Arsenophonus in midgut was greater than that in the whole body. The relative abundance of Bartonella in fully engorged adults was far greater than those in newly hatched, unfed adults. The relative abundance of Wolbachia was highest in the whole body of newly hatched, unfed adults. Seventeen bacterial species were identified in all experimental samples. Bartonella chomelii, Streptococcus hyointestinalis and Escherichia coli were the first species reported in M. ovinus.

First determination of DNA virus and some additional bacteria from Melophagus ovinus (sheep ked) in Tibet, China

“…M. ovinus has a broad geographical distribution and is reported in Europe [e.g., Czechia ( Chrudimský et al, 2012 ; Nováková et al, 2015 ; Rudolf et al, 2016 ), Hungary ( Hornok et al, 2011 ), Slovakia ( Hubálek et al, 1986 ), Croatia ( Martinković et al, 2012 ), St Kilda ( Gibson et al, 2010 ), France ( Halos et al, 2004 ), Poland ( Werszko et al, 2021 ), Russia ( Litov et al, 2021 )], Africa [e.g., Ethiopia ( Kumsa et al, 2012 , 2014 ; Seyoum et al, 2015 ; Hadgu et al, 2021 ), Algeria ( Boucheikhchoukh et al, 2019 ), Kenya ( Liu et al, 2016 )], Oceania [e.g., Australia ( Liu et al, 2016 ; Litov et al, 2021 ), New Zealand ( Liu et al, 2016 )], North America [e.g., United States ( Halos et al, 2004 ; Kosoy et al, 2004 , 2016 )], South America [e.g., Peru ( Flores-Mendoza et al, 2021 )] and Asia [e.g., Turkey ( Payzin, 1953 ), Mongolia, India, Japan ( Liu et al, 2016 )]. In recent years, M. ovinus has frequently appeared in China, Asia in Tibet ( Chu et al, 2011 ), Gansu ( Duan et al, 2017 , 2020 ; Tang et al, 2018 ; Zhao et al, 2020 ), Xinjiang ( Liu et al, 2016 , 2017 , 2019 ; Zhao et al, 2018 , 2020 ), Sichuan ( Hao et al, 2020 ), and Qinghai ( Zhang et al, 2021 ).…”

“…( Hornok et al, 2011 ), R. raoultii , R. slovaca ( Liu et al, 2016 ), R. melophagi ( Arkwright et al, 1919 ; Cowdry, 1923 ; Hertig and Wolbach, 1924 ; Kligler and Aschner, 1931 ; Rudolf et al, 2016 ), Bartonella spp. ( Halos et al, 2004 ; Reeves et al, 2006 ; Kumsa et al, 2014 ; Duan et al, 2020 ; Werszko et al, 2021 ), Ba. melophagi ( Halos et al, 2004 ; Kosoy et al, 2004 , 2016 ; Bemis and Kania, 2007 ; Kumsa et al, 2014 ; Rudolf et al, 2016 ; Liu et al, 2018 ; Boucheikhchoukh et al, 2019 ; Flores-Mendoza et al, 2021 ), Ba.…”

“…melophagi variants variants, Ba. chomelii ( Duan et al, 2020 ), Ba. schoenbuchensis ( Halos et al, 2004 ), Wolbachia sp.…”

“…schoenbuchensis ( Halos et al, 2004 ), Wolbachia sp. ( Duan et al, 2017 , 2020 ; Liu et al, 2018 ; Boucheikhchoukh et al, 2019 ), Trypanosoma spp. ( Werszko et al, 2021 ), Tr.…”

Tandem mass tag labeled quantitative proteomic analysis of differential protein expression on total alkaloid of Aconitum flavum Hand.-Mazz. against melophagus ovinus

“…Besides, the most serious harm of Melophagus ovinus is that it can be used as a vector to spread bluetongue virus ( 1 ), Trypanosoma ( 2 ), Bartonella ( 3 ), Rickettsia ( 4 ), Acinetobacter ( 5 ), and other pathogens. Among them, bluetongue is one of the most susceptible sheep infectious diseases, with an incidence rate of 76% ( 6 ), causing great harm to animal husbandry.…”