Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus

Baños-Lara, Ma. Del Rocío; Zabaleta, Jovanny; Garai, Jone; Baddoo, Melody; Guerrero-Plata, Antonieta

doi:10.1186/s13104-018-3541-0

Cited by 23 publications

(25 citation statements)

References 42 publications

(46 reference statements)

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“…For example, Mn 2+ ion uptake in neuron cells exposed to MnCl2 was shown to trigger upregulation of miR-6087 and miR-7704 (He et al 2017). Hsa-miR-7704 has also been validated as a miRNA that is upregulated by more than 20-fold in virus-infected dendritic cells (Baños-Lara et al 2018). MiR-342-3p was observed to be downregulated in A549 cells exposed to chitosan conjugated gold nanoparticles (Choi et al 2018).…”

Section: Discussionmentioning

confidence: 97%

Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential

et al. 2019

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After over a decade of nanosafety research, it is indisputable that the vast majority of nano-sized particles induce a plethora of adverse cellular responses-the severity of which is linked to the material's physicochemical properties. Differentiated THP-1 cells were previously exposed for 6 hours (6h) and 24 hours (24h) to silver, titanium dioxide and zinc oxide nanoparticles at the maximum molar concentration at which no more than 15% cellular cytotoxicity was observed. All three nanoparticles differed in extent of induction of biological pathways corresponding to immune response signaling and metal ion homeostasis. In the current study, we integrated gene and miRNA expression profiles from the same cells to propose miRNA biomarkers of adverse exposure to metal-based nanoparticles. We employed RNA sequencing together with a quantitative strategy that also enables analysis of the overlooked repertoire of length and sequence miRNA variants called isomiRs. Whilst only modest changes in expression was observed within the first 6h of exposure, the miRNA/isomiR (miR) profiles of each nanoparticle was unique. Via canonical correlation and pathway enrichment analyses, we identified a co-regulated miR-mRNA cluster, predicted to be highly relevant for cellular response to metal ion homeostasis. These miRs were annotated to be canonical or variant isoforms of Hsa-miR-142-5p,-342-3p,-5100,-6087,-6894-3p and-7704. Hsa-miR-5100 was differentially expressed in response to each nanoparticle in both the 6h and 24h exposures. Taken together, this co-regulated miR-mRNA cluster could represent potential biomarkers of sub-toxic metal-based nanoparticle exposure.

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Section: Discussionmentioning

confidence: 97%

Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential

et al. 2019

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“…Importantly, hMPV infection in DCs also induces the expression of several microRNAs (miRs) such as hsa-miR-182-5p and hsa-miR-4634. Although several microRNAs are believed to be known regulators of DC functions, including maturation, antigen presentation, and cytokine production, no link between the target genes of the hMPV-induced miRNAs and DC function has been found [120,121].…”

Section: Dendritic Cells (Dcs)mentioning

confidence: 99%

Cell-Mediated Responses to Human Metapneumovirus Infection

Ballegeer

Saelens

2020

Viruses

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Viruses are the most common cause of acute respiratory tract infections (ARTI). Human metapneumovirus (hMPV) frequently causes viral pneumonia which can become life-threatening if the virus spreads to the lungs. Even though hMPV was only isolated in 2001, this negative-stranded RNA virus has probably been circulating in the human population for many decades. Interestingly, almost all adults have serologic evidence of hMPV infection. A well-established host immune response is evoked when hMPV infection occurs. However, the virus has evolved to circumvent and even exploit the host immune response. Further, infection with hMPV induces a weak memory response, and re-infections during life are common. In this review, we provide a comprehensive overview of the different cell types involved in the immune response in order to better understand the immunopathology induced by hMPV. Such knowledge may contribute to the development of vaccines and therapeutics directed against hMPV.

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“…A recent study by Baños-Lara et al showed a differential miRNA pattern expression in Monocyte derived DCs (MoDCs) induced by hMPV- and hRSV-infection. Therein, they report that hMPV induces a higher expression of has-miR-185–5p, a miRNA that affects different genes such as FOXO1, PDCD4 and RECK, among others; but the effect in the dysregulation of these genes is not reported ( 89 ). On the other hand, hRSV induces the expression of miR-4448, that is associated with the Akt/mTOR pathway, inhibiting apoptosis and, as a consequence, increasing cell survivor ( 90 ).…”

Section: Tlrs and Their Effects Over The Immune Response Induced By Hmentioning

confidence: 99%

Human Metapneumovirus: Mechanisms and Molecular Targets Used by the Virus to Avoid the Immune System

et al. 2018

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Human metapneumovirus (hMPV) is a respiratory virus, first reported the year 2001. Since then, it has been described as one of the main etiological agents that causes acute lower respiratory tract infections (ALRTIs), which is characterized by symptoms such as bronchiolitis, wheezing and coughing. Susceptible population to hMPV-infection includes newborn, children, elderly and immunocompromised individuals. This viral agent is a negative-sense, single-stranded RNA enveloped virus, that belongs to the Pneumoviridae family and Metapneumovirus genus. Early reports—previous to 2001—state several cases of respiratory illness without clear identification of the responsible pathogen, which could be related to hMPV. Despite the similarities of hMPV with several other viruses, such as the human respiratory syncytial virus or influenza virus, mechanisms used by hMPV to avoid the host immune system are still unclear. In fact, evidence indicates that hMPV induces a poor innate immune response, thereby affecting the adaptive immunity. Among these mechanisms, is the promotion of an anergic state in T cells, instead of an effective polarization or activation, which could be induced by low levels of cytokine secretion. Further, the evidences support the notion that hMPV interferes with several pattern recognition receptors (PRRs) and cell signaling pathways triggered by interferon-associated genes. However, these mechanisms reported in hMPV are not like the ones reported for hRSV, as the latter has two non-structural proteins that are able to inhibit these pathways. Several reports suggest that viral glycoproteins, such as G and SH, could play immune-modulator roles during infection. In this work, we discuss the state of the art regarding the mechanisms that underlie the poor immunity elicited by hMPV. Importantly, these mechanisms will be compared with those elicited by other common respiratory viruses.

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Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus

Cited by 23 publications

References 42 publications

Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential

Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential

Cell-Mediated Responses to Human Metapneumovirus Infection

Human Metapneumovirus: Mechanisms and Molecular Targets Used by the Virus to Avoid the Immune System

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