Comparative Analysis of Morphometric Characteristics and Mucous Cell Distribution between Pangasius hypophthalmus and Clarias batrachus

The Effects of Stocking Density and Food Deprivation on Mucous Cells and Lysozyme Activity in the Skin and Gills of Silver Catfish

“…A lower count of mucosal cells was observed in the abdomen. However, an inverse pattern was found in Philippine catfish, where the highest number of mucosal cells was found in the head region and the lowest in the tail [22].…”

“…However, it is recognized that cutaneous mucus is considered the first line of defense against infections through the skin epidermis [1]. As proposed for Atlantic salmon (Salmo salar) [26], quantification of cutaneous goblet cells in silver catfish was observed at three collection points above the left lateral line and three collection points below the lateral line, while in striped catfish and Philippine catfish, the observed regions included barbels and dorsal and ventral parts of the head and the abdomen [22]. Therefore, adding to the proposed model for Atlantic salmon [26], an additional collection point was chosen that was located caudally to the operculum, which shows a similar cellularity to the other body regions observed in silver catfish.…”

“…The surface of the epidermis, in general, is covered by a mosaic pavement of polygonal epithelial cells with varying dimensions and irregular distribution [25]. It is also understandable that the distribution of mucous cells in a small area of the epidermis is not always uniform [22]. However, it is recognized that cutaneous mucus is considered the first line of defense against infections through the skin epidermis [1].…”

“…Samples were individually placed in 15 mL tubes containing 10% formaldehyde solution to facilitate subsequent routine histological processing. Upon paraffin embedding, 5 µm thick sections of both skin and gill tissues were subjected to PAS and Alcian Blue staining methods to analyze mucus-secreting cells [3,4,22]. According to [22], images of various regions were observed under an optical microscope, where an analysis of mucus-secreting cells was conducted in 15 to 25 fields for each section of the skin and gills of each sampled fish.…”

“…analyze mucus-secreting cells [3,4,22]. According to [22], images of various regions were observed under an optical microscope, where an analysis of mucus-secreting cells was conducted in 15 to 25 fields for each section of the skin and gills of each sampled fish.…”