Comparative analysis of mouse embryonic palatal mesenchymal cells isolated by two primary culture methods

Wang, Xiaotong; Xia, Peng; Chen, Jing; Wang, Yijia; Zhao, Xige; Li, Tianli; Du, Juan

doi:10.1016/j.tice.2022.101783

Cited by 3 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MEPM cells were derived from E13.5 ICR pregnant mice as in our previous study [ 53 ] (purchased from Sibeifu Company, Beijing, China). The palatal shelves were isolated and then digested with 0.25% trypsin (25200-072, Gibco Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C for 20 min to obtain MEPM cells.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Cxcr4 Disrupts Mouse Embryonic Palatal Mesenchymal Cell Migration and Induces Cleft Palate Occurrence

Zheng

Zhao

Wang

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Many processes take place during embryogenesis, and the development of the palate mainly involves proliferation, migration, osteogenesis, and epithelial–mesenchymal transition. Abnormalities in any of these processes can be the cause of cleft palate (CP). There have been few reports on whether C-X-C motif chemokine receptor 4 (CXCR4), which is involved in embryonic development, participates in these processes. In our study, the knockdown of Cxcr4 inhibited the migration of mouse embryonic palatal mesenchymal (MEPM) cells similarly to the use of its inhibitor plerixafor, and the inhibition of cell migration in the Cxcr4 knockdown group was partially reversed by supplementation with C-X-C motif chemokine ligand 12 (CXCL12). In combination with low-dose retinoic acid (RA), plerixafor increased the incidence of cleft palates in mice by decreasing the expression of Cxcr4 and its downstream migration-regulating gene Rac family small GTPase 1 (RAC1) mediating actin cytoskeleton to affect lamellipodia formation and focal complex assembly and ras homolog family member A (RHOA) regulating the actin cytoskeleton to affect stress fiber formation and focal complex maturation into focal adhesions. Our results indicate that the disruption of cell migration and impaired normal palatal development by inhibition of Cxcr4 expression might be mediated through Rac1 with RhoA. The combination of retinoic acid and plerixafor might increase the incidence of cleft palate, which also provided a rationale to guide the use of the drug during conception.

show abstract

Section: Methodsmentioning

confidence: 99%

Inhibition of Cxcr4 Disrupts Mouse Embryonic Palatal Mesenchymal Cell Migration and Induces Cleft Palate Occurrence

Zheng

Zhao

Wang

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The method of MEPM cell culture was according to Wang’ report. 18 The MEPM cells were cultured in flasks with DMEM (Dulbecco’s Modified Eagle’s medium)/F12 medium (Hyclon, Logan, Utah, USA) supplemented with 10% fetal calf serum (Sijiqing, Hangzhou, China). The MEPM cells were placed in a humidified incubator at 37°C with 5% CO 2 atmosphere with media replaced every other day.…”

Section: Methodsmentioning

confidence: 99%

“…The gene expression was regulated by non-coding RNAs (ncRNAs) and revealed the full transcriptome level through the regulatory network of the ceRNAs. 18 Many circRNAs mediated their target protein-coding genes. CircRNA4451 may be regarded as centers and targeted tgfbr2 through TGF-beta signaling pathway.…”

Section: Predicted Function Of Circrnasmentioning

confidence: 99%

Identification and characterization of differentially expressed circRNA in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cleft palate

et al. 2023

View full text Add to dashboard Cite

Various circular RNAs (circRNAs) are novel class of non-coding RNAs, which are pervasively transcribed in the genome. CircRNAs play important roles in human, animals and plants. Up to now, there was no report regarding circRNAs of cleft palate by 2,3,7,8-tetrachlorodibenzo-pdioxin (TCDD) induce. The present study screened identification and characterization of differential expressed-circRNAs in TCDD-induced cleft palate. 6903 circRNAs candidates came from cleft palates. Among them, 3525 circRNAs are up-regulation, and 3378 circRNAs are down-regulation by TCDD induce. The cluster and GO analysis found that circRNAs involved in biological process, cellular component, and molecular function. Through the analysis of KEGG Pathway, circRNAs made functions via classical signaling pathway in cleft palate, such as TGF-beta signaling pathway, BMP signal pathway, MAPK signaling pathway. In addition, we found down-regulated circRNA224, circRNA3302 and up-regulated circRNA5021 targeted tgfbr3, but up-regulated circRNA4451 targeted tgfbr2. circRNA4451 may make functions through TGF-beta signaling pathway. These results suggested that many different circRNAs may make important role in TCDD-induced cleft palate, which provided a theoretical basis for further research.

show abstract

Osteogenic microenvironment affects palatal development through glycolysis

et al. 2023

View full text Add to dashboard Cite

Comparative analysis of mouse embryonic palatal mesenchymal cells isolated by two primary culture methods

Cited by 3 publications

References 46 publications

Inhibition of Cxcr4 Disrupts Mouse Embryonic Palatal Mesenchymal Cell Migration and Induces Cleft Palate Occurrence

Inhibition of Cxcr4 Disrupts Mouse Embryonic Palatal Mesenchymal Cell Migration and Induces Cleft Palate Occurrence

Identification and characterization of differentially expressed circRNA in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cleft palate

Osteogenic microenvironment affects palatal development through glycolysis

Contact Info

Product

Resources

About