Comparative Analysis of Polylocus Spectra of ISSR-PCR Markers in Dogs, Jackals And Wolves

Abstract

The problems of the relationship between the three representatives of the canidae family (Canidae) based on the example of a domestic dog (Canis lupus familiaris), a wolf (Canis lupus) and a jackal (Canis aureus) are considered. A study using ISSR-PCR on di-and trinucleotide primers (AG) 9C, (GA) 9C and (GAG) 6C, showed that jackals are more genetically diverse than wolves and dogs. According to the spectra of amplification products and the presence of common fragments, jackals are genetically closer to dogs than to wolves. Based on the genotyping of 53 loci, it can be concluded that in the genome of the jackal, the DNA segments flanked by inverted microsatellite repeats are more common than those observed in the genomes of wolves and the domestic dog. The results of studies using ISSR-PCR markers, indicating a high genetic heterogeneity of the jackal population, are consistent with the published data obtained by genotyping at microsatellite loci, as well as the polymorphism of the D-loop of mitochondrial DNA, which also indicate a more pronounced genetic variability Jackals in comparison with dogs and wolves.