Comparative analysis of salt-induced changes in the root proteome of two accessions of the halophyte Cakile maritima

Belghith, Ikram; Senkler, Jennifer; Hildebrandt, Tatjana M.; Abdelly, Chédly; Braun, Hans‐Peter; Debez, Ahmed

doi:10.1016/j.plaphy.2018.06.029

Cited by 20 publications

(11 citation statements)

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“…The response to salt stress also differed between populations. It has been reported that C. maritima has a great genetic diversity due to adaptation to different ecological conditions, and, consequently, its populations may have different optimal temperatures and photoperiods for germination (Debez et al 2013;Belghith et al 2018) and different levels of salt sensitivity. Differences in the germination response of C. maritima to NaCl have been mainly attributed to different climatic conditions of collection sites.…”

Section: Discussionmentioning

confidence: 99%

“…Although its growth at vegetative stage and seed production are enhanced at moderate salinity (NaCl 50-100 mM; Davy et al 2006;Debez et al 2006Debez et al , 2012, this species is salt-sensitive during the germination phase (Debez et al 2004(Debez et al , 2008(Debez et al , 2018Megdiche et al 2007;Ghars et al 2009). While the ecology of the species has been investigated in depth (Davy et al 2006;Megdiche et al 2008;Debez et al 2013;Ben Hamed-Laouti et al 2016;Houmani and Corpas 2016;Belghith et al 2018), the response to salinity has been mainly analysed for Tunisian populations (Debez et al 2004(Debez et al , 2008(Debez et al , 2012(Debez et al , 2018Megdiche et al 2007;Ghars et al 2009), while other areas of the Mediterranean Basin received less attention. Moreover, the interaction with other environmental factors such as temperature and salinity has often been overlooked, except for England, where GA 3 -treated seeds were found to be less sensitive to salinity at the optimal germination temperature of 20°C (Ignaciuk and Lee 1980).…”

Section: Introductionmentioning

confidence: 99%

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Germination responses of Mediterranean populations of Cakile maritima to light, salinity and temperature

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Germination responses of Mediterranean populations of Cakile maritima to light, salinity and temperature

et al. 2018

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“…Similar results were verified in a study on the effects of NaCl on Broussonetia papyrifera to show the different activities of SOD, POD, and CAT in leaves, stems, and roots accompanying up/down-regulated expression of SOD, POD, and CAT isoenzymes caused by 50, 100, and 150 mM NaCl treatments ( Zhang et al, 2013 ). Heat-shock family proteins such as sHsp, Hsp60, Hsp70, Hsp90, Hsp100 and HSF can regulate hormones, kinases, the cell cycle, the redox state, antioxidant activity and osmolytes for stress tolerance ( Wang et al, 2004 ) and the study reported that two salt-responsive heat-shock proteins (Hsp60 and Hsp70) were identified in the roots of the halophyte Cakile maritima under 100 and 300 mM NaCl treatments ( Belghith et al, 2018 ). The O2-evolving complex 33 kD family protein in our research was downregulated (0.23 ± 0.17) and affected PSII.…”

Section: Discussionmentioning

confidence: 99%

The photo-inhibition of camphor leaves (Cinnamomum camphora L.) by NaCl stress based on physiological, chloroplast structure and comparative proteomic analysis

Yue

Shi

Zhang

et al. 2020

PeerJ

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Background The distribution and use of camphor (Cinnamomum camphora L.) trees are constrained by increasing soil salinity in south-eastern China along the Yangtze River. However, the response mechanism of this species to salinity, especially in team of photosynthesis, are unknown. Methods Here, we analysed themorphological, physiological, ultrastructural, and proteomic traits of camphor seedlings under NaCl (103.45 mM) treatment in pot experiments for 80 days. Results The growth was limited because of photosynthetic inhibition, with the most significant disturbance occurring within 50 days. Salinity caused severe reductions in the leaf photosynthetic rate (An), stomatal conductance (gs), maximal chlorophyll fluorescence (Fm), maximum quantum yield of PSII (Fv/Fm), non-photochemical quenching (NPQ), relative quantum efficiency of PSII photochemistry (ΦPSII), photochemical quenching coefficient (qP) and photo-pigment contents (chlorophyll a (Cha), chlorophyll b (Chb), total chlorophyll (Chl)); weakened the antioxidant effects, including those of malondialdehyde (MDA), superoxide dismutase (SOD) and peroxidase (POD); and injured chloroplasts. The physiologicalresults indicated that the main reason for photo-inhibition was oxidative factors induced by NaCl. The proteomic results based on isobaric tags for relative and absolute quantitation (iTRAQ) further confirmedthat photosynthesis was the most significant disrupted process by salinity (P < 0.01) and there were 30 downregulated differentially expression proteins (DEPs) and one upregulated DEP related to restraint of the photosynthetic system, which affected photosystem I, photosystem II, the Cytochrome b6/f complex, ATP synthase and the light-harvesting chlorophyll protein complex. In addition, 57 DEPs were related to photo-inhibition by redox effect and 6 downregulated DEPs, including O2 evolving complex 33kD family protein (gi—224094610) and five other predicted proteins (gi—743921083, gi—743840443, gi—743885735, gi—743810316 and gi—743881832) were directly affected. This study provides new proteomic information and explains the possible mechanisms of photo-inhibition caused by salinity on C. camphor.

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“…V-H + -PPase and V-H + -ATPase are two proton transport systems in the plant vacuolar membrane; these systems create electrochemical potential gradients across the tonoplast and transport many metabolites [38,39]. These proton pumps have been identified in several proteomic studies and are also considered as important salt-responsive marker proteins [37,40,41,42]. Overexpression of V-H + -PPase or V-H + -ATPase resulted in enhanced salt tolerance in transgenic tobacco [43] or Broussonetia papyrifera [44].…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.)

Wang

Feng

et al. 2018

IJMS

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Salinity is one of the major abiotic stress factors that limit plant growth and crop yield worldwide. To understand the molecular mechanisms and screen the key proteins in response of sugar beet (Beta vulgaris L.) to salt, in the present study, the proteomics of roots and shoots in three-week-old sugar beet plants exposed to 50 mM NaCl for 72 h was investigated by isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology. The results showed that 105 and 30 differentially expressed proteins (DEPs) were identified in roots and shoots of salt-treated plants compared with untreated plants, respectively. There were 46 proteins up-regulated and 59 proteins down-regulated in roots; and 13 up-regulated proteins and 17 down-regulated proteins found in shoots, respectively. These DEPs were mainly involved in carbohydrate metabolism, energy metabolism, lipid metabolism, biosynthesis of secondary metabolites, transcription, translation, protein folding, sorting, and degradation as well as transport. It is worth emphasizing that some novel salt-responsive proteins were identified, such as PFK5, MDH, KAT2, ACAD10, CYP51, F3H, TAL, SRPR, ZOG, V-H+-ATPase, V-H+-PPase, PIPs, TIPs, and tubulin α-2/β-1 chain. qRT-PCR analysis showed that six of the selected proteins, including BvPIP1-4, BvVP and BvVAP in root and BvTAL, BvURO-D1, and BvZOG in shoot, displayed good correlation between the expression levels of protein and mRNA. These novel proteins provide a good starting point for further research into their functions using genetic or other approaches. These findings should significantly improve the understanding of the molecular mechanisms involved in salt tolerance of sugar beet plants.

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Comparative analysis of salt-induced changes in the root proteome of two accessions of the halophyte Cakile maritima

Cited by 20 publications

References 35 publications

Germination responses of Mediterranean populations of Cakile maritima to light, salinity and temperature

Germination responses of Mediterranean populations of Cakile maritima to light, salinity and temperature

The photo-inhibition of camphor leaves (Cinnamomum camphora L.) by NaCl stress based on physiological, chloroplast structure and comparative proteomic analysis

iTRAQ-Based Comparative Proteomic Analysis Provides Insights into Molecular Mechanisms of Salt Tolerance in Sugar Beet (Beta vulgaris L.)

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