Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants

Acemi, Arda; Bayrak, Bahar; Çakır, Merve; Demiryürek, Elif; Gün, Ece; Gueddari, Nour Eddine El; Özen, Fazıl

doi:10.1007/s11627-018-9915-0

Cited by 30 publications

(25 citation statements)

References 38 publications

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“…The biologic effects of biopolymers may differ according to their chemical properties. Thus their DP and DA may have critical roles, especially in plant production (5). The name "chitosan" is a general name used to describe the deacetylated form of chitin.…”

Section: Discussionmentioning

confidence: 99%

“…The seeds of Ipomoea purpurea (L.) Roth supplied from Vilmorin Garden, Turkey, were cultured in vitro to supply an adequate number of plant material for shoot multiplication. Surface sterilization of the seeds was done in 1% (w/v) sodium hypochlorite (NaOCl) solution with gentle shaking for three minutes, as described by Acemi et al (5). The seeds were then transferred onto Murashige & Skoog (MS) medium (11) and incubated for 30 days ( Fig.…”

Section: Plant Materials and Culture Establishmentmentioning

confidence: 99%

“…1B). Nodal explants with at least one node were taken from the multiplied shoots and further cultured in MS medium, supplemented either with chitosan oligomer mixture, with DP ranging from 2 to 15 or polymer (DP 70) at three different concentrations (5,10, and 20 mg L −1 ) as previously described by Acemi et al (5). The DA of each chitosan variant was 10%.…”

Section: Plant Materials and Culture Establishmentmentioning

confidence: 99%

“…Chitosan, which has been shown to be a natural alternative to commonly used synthetic PGRs (5), is the deacetylated form of the biopolymer of chitin. Chitin is found in the shells of members of the Crustaceae and the carapaces of insects, in the cell walls of fungi and in some algae (6).…”

Section: Introductionmentioning

confidence: 99%

“…Chitosans have been used to enhance seed germination rate, to induce lateral branching and earlier flowering and to extend vase-life in ornamental plants. In these reports, in vitro effects of chitosans have been mainly investigated based on morphometric parameters such as plant shoot and root length, and the number of shoots, roots and leaves (5,7,8). Besides these parameters, basic physiological parameters such as photosynthetic pigments, protein, and relative water contents could also be useful in giving an insight into the effects of different chitosans, since these parameters are related to the visual appearance of ornamentals.…”

Section: Introductionmentioning

confidence: 99%

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Polymerization degree-dependent changes in the effects of in vitro chitosan treatments on photosynthetic pigment, protein, and dry matter contents of Ipomoea purpurea

Acemi

2019

The EuroBiotech Journal

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Morning Glory (Ipomoea purpurea (L.) Roth.) is a climbing plant known for its ornamental properties and ease of cultivation in temperate climates. Quality and colour of flowers and leaves, especially in the production of ornamentals, are important parameters both for producers and for customers. This study aimed to investigate the changes in photosynthetic pigment, protein and dry matter content of in vitro-propagated I. purpurea following chitosan treatment with different polymerization degrees (DP) and to determine the indirect effect of this biopolymer on leaves of the plant. Nodal explants of I. purpurea were cultured in medium supplemented with 5, 10 and 20 mg L−1 concentrations of a chitosan oligomers mixture with a variable degree of polymerization (DP) ranging from 2 to 15 or chitosan polymer with DP of 70. It was found that both oligomeric and polymeric chitosan treatments increased chlorophyll-a contents in the leaves when compared to the chitosan-naïve control group. Polymeric chitosan stimulated chlorophyll-b and carotenoid synthesis more effectively than the oligomer mixture. Also, 10 mg L−1 polymeric chitosan better triggered total protein production and plant dry matter content in I. purpurea. The results of this study showed that, due to their stimulatory effects on photosynthetic pigment, protein and plant dry matter production, chitosan oligomers at low concentration and polymers at moderate concentration might be considered as safe and natural biostimulants for ornamental plants which could affect the plant’s attractiveness and commercial success.

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Section: Discussionmentioning

confidence: 99%

Section: Plant Materials and Culture Establishmentmentioning

confidence: 99%

Section: Plant Materials and Culture Establishmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polymerization degree-dependent changes in the effects of in vitro chitosan treatments on photosynthetic pigment, protein, and dry matter contents of Ipomoea purpurea

Acemi

2019

The EuroBiotech Journal

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Biostimulation and Toxicity

Benavides-Mendoza

González-Moscoso

Ojeda-Barrios

et al. 2021

Nanotechnology in Plant Growth Promotion and Protection

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Harvey

2021

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.

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Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants

Cited by 30 publications

References 38 publications

Polymerization degree-dependent changes in the effects of in vitro chitosan treatments on photosynthetic pigment, protein, and dry matter contents of Ipomoea purpurea

Polymerization degree-dependent changes in the effects of in vitro chitosan treatments on photosynthetic pigment, protein, and dry matter contents of Ipomoea purpurea

Biostimulation and Toxicity

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

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