Comparative Analysis of the Rats’ Gut Microbiota Composition in Animals with Different Ginsenosides Metabolizing Activity

Dong, Weiwei; Xuan, Fang-Ling; Zhong, Fei-Liang; Jiang, Jun; Wu, Songquan; Li, Donghao; Quan, Lin-Hu

doi:10.1021/acs.jafc.6b04848

Cited by 43 publications

(28 citation statements)

References 36 publications

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“…16 Saponins regulate gut microbiota composition and alleviate intestinal inflammatory environment. 28 Here we demonstrated that HFD increased the abundance of Desulfovibrio genus and clostridiales order, and elevated the levels of LPS and DCA in the feces and serums. Conversely, GA modulated gut microbiota and decreased the levels of LPS and DCA in the feces, and thereby prevented pre-metastatic niche formation and metastasis.…”

Section: Discussionmentioning

confidence: 60%

“…Saponins, a novel type of plant-derived secondary metabolites, modulate gut microbiota composition and exhibit anti-metastasis activities in multiple tumors including lung adenocarcinoma, hepatocellular carcinoma, and leukemia. 27,28 Glycyrrhizic acid (GA), a triterpenoid saponin glycoside from the licorice roots, inhibits tumor growth and B16 melanoma pulmonary metastasis. 29,30 GA also ameliorates HFD-induced obesity and non-alcoholic fatty liver disease in murine models.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis

et al. 2019

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High-fat diet (HFD) promotes lung pre-metastatic niche formation and metastasis. Thus, there is an urgent need to identify the underlying mechanisms and develop strategies to overcome them. Here we demonstrate that glycyrrhizic acid (GA) prevents HFDenhanced pre-metastatic niche formation and metastasis through gut microbiota. GA reduced HFD-enhanced myeloid-derived suppressor cell recruitment, pro-metastatic protein S100A8/A9 expression and metastasis burden of 4T1 breast cancer and B16F10 melanoma, accompanied by gut microbiota alteration and colonic macrophage polarization far away the M1-like phenotype. These parameters were greatly decreased by treatment with antibiotics, recolonization of Desulfovibrio vulgaris and Clostridium sordellii, and administration of lipopolysaccharide or deoxycholic acid. Macrophage depletion attenuated HFD-enhanced pre-metastatic niche formation and metastasis, but failed to further affect the effects of GA. Mechanistically, counteraction of HFD-enhanced gut microbiota dysbiosis by GA inhibited Gr-1 + myeloid cell migration and S100A8/A9 expression through decreasing the proportion of M1-like macrophages and their production of CCL2 and TNF-α in the colons via LPS/HMGB1/NF-κB signaling inactivation. Together, targeting the gut microbiota by GA to modulate colonic macrophages could be a novel strategy for the prevention of HFDenhanced pre-metastatic niche formation and metastasis.

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Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis

et al. 2019

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“…In general, deglycosylation of natural products is regulated by enterobacterial hydrolases. To further elucidate whether differences in the composition of intestinal microbiota result in different activities of hydrolytic enzymes and to analyze the relationships between microbiota enzymatic activity and metabolism, we measured the intestinal microbial glucosidase activities in each group (Dong et al, ). The glycosidase activities in the two model groups were lower than those in the control group, and there was a significant difference between the OACS and ATM groups.…”

Section: Discussionmentioning

confidence: 99%

Changes in intestinal microbiota affect metabolism of ginsenoside Re

Zhang

Qin

et al. 2018

Biomedical Chromatography

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Ginsenoside Re, an active ingredient in Panax ginseng, is widely used as a therapeutic and nutriment. The intestinal microbiota plays crucial roles in modulating the pharmacokinetics and pharmacological actions of ginsenoside Re. The aim of this study was to explore the relationship between bacterial community variety and the metabolic profiles of ginsenoside Re. We developed two models with intestinal dysbacteriosis: a pseudo-germ-free model induced by a nonabsorbable antimicrobial mixture (ATM), and Qi-deficiency model established via over-fatigue and acute cold stress (OACS). First, the bacterial community structures in control, ATM and OACS rats were compared via 16S ribosomal RNA amplicon sequencing. Then, the gut microbial metabolism of ginsenoside Re was assessed qualitatively and quantitatively in the three groups by UPLC-Q-TOF/MS and HPLC-TQ-MS, respectively. Ten metabolites of ginsenoside Re were detected and tentatively identified, three of which were novel. Moreover, owing to significant differences in bacterial communities, deglycosylated products, as the main metabolites of ginsenoside Re, were produced at lower levels in ATM and OACS models. Importantly, the levels of these deglycosylated metabolites correlated with alterations in Prevotella, Lactobacillus and Bacteroides populations, as well as glycosidase activities. Collectively, biotransformation of ginsenoside Re is potentially influenced by regulation of the composition of intestinal microbiota and glycosidase activities.

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“…Microbial phylotype richness was estimated by Chao and Ace, and the diversity of the bacterial community was revealed by Shannon [ 17 ]. As shown in Table 1 , there was a significant overall decrease in the richness and the diversity of gut microbiota in the mice fed by HFD ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

Effect of A Polyphenol-Rich Canarium album Extract on the Composition of the Gut Microbiota of Mice Fed a High-Fat Diet

Zhang

Guo

et al. 2018

Molecules

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This study investigated the influence of Canarium album extract (CAext) on intestinal microbiota composition of mice fed a high-fat diet (HFD). Kun Ming (KM) mice were fed either a normal chow diet or a HFD for six weeks. At the seventh week, HFD-fed mice were gavaged daily with saline, or a different dose of CAext for four weeks, respectively. Then, the composition of the gut microbiota was analyzed by high-throughput sequencing technology. Analysis of fecal microbial populations, grouped by phyla, showed significant increases of Firmicutes and Verrucomicrobia, but a decrease of Bacteroidetes in all CAext-fed mice. Particularly, CAext gavage in a low dose or a medium dose caused a significant increase in the proportion of Akkermansia. These findings suggested that CAext can alter the gut microbiota composition of HFD-fed mice, and had a potential prebiotic effects on Akkermansia.

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Comparative Analysis of the Rats’ Gut Microbiota Composition in Animals with Different Ginsenosides Metabolizing Activity

Cited by 43 publications

References 36 publications

Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis

Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis

Changes in intestinal microbiota affect metabolism of ginsenoside Re

Effect of A Polyphenol-Rich Canarium album Extract on the Composition of the Gut Microbiota of Mice Fed a High-Fat Diet

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