Comparative analysis of the self‐incompatibility (<i>S</i>‐) locus region of <i>Prunus mume</i>: identification of a pollen‐expressed F‐box gene with allelic diversity

Entani, Tetsuyuki; Iwano, Megumi; Shiba, Hiroshi; Che, Fang‐Sik; Isogai, Akira; Takayama, Seiji

doi:10.1046/j.1365-2443.2003.00626.x

Cited by 300 publications

(405 citation statements)

References 52 publications

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“…In the Solanaceae, Asteraceae, Convulvulaceae, Plantaginaceae and Rosaceae, SI is mediated by the reaction of a secreted pistil-expressed RNase (SRNase) and a pollen-expressed F-box protein, SLF (Murfett et al, 1994;Sijacic et al, 2004). Both female (for example, Ushijima et al, 1998) and male (for example, Entani et al, 2003) components exhibit high sequence diversity, with novel haplotypes possibly derived by recombination (Wang et al, 2001) or gene duplication: in the case of SLF, more than five different subgroups have been detected and several SLF-like genes are found in close linkage at the S locus and are co-expressed in pollen (Kubo et al, 2010). In the tomato, the SI system is an important mediator of interspecific unilateral incompatibility (Li and Chetelat, 2010), once again highlighting the importance of supergenes in ecological speciation.…”

Section: Self-incompatibility and Heterostylymentioning

confidence: 99%

Supergenes and their role in evolution

2014

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Adaptation is commonly a multidimensional problem, with changes in multiple traits required to match a complex environment. This is epitomized by balanced polymorphisms in which multiple phenotypes co-exist and are maintained in a population by a balance of selective forces. Consideration of such polymorphisms led to the concept of the supergene, where alternative phenotypes in a balanced polymorphism segregate as if controlled by a single genetic locus, resulting from tight genetic linkage between multiple functional loci. Recently, the molecular basis for several supergenes has been resolved. Thus, major chromosomal inversions have been shown to be associated with polymorphisms in butterflies, ants and birds, offering a mechanism for localised reduction in recombination. In several examples of plant self-incompatibility, the functional role of multiple elements within the supergene architecture has been demonstrated, conclusively showing that balanced polymorphism can be maintained at multiple coadapted and tightly linked elements. Despite recent criticism, we argue that the supergene concept remains relevant and is more testable than ever with modern molecular methods.

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Section: Self-incompatibility and Heterostylymentioning

confidence: 99%

Supergenes and their role in evolution

2014

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“…The S-pollen gene encodes an SLF or SFB (SLF/SFB; for S-locus F-box) protein, which is a member of the Fbox protein family (Entani et al, 2003;Sijacic et al, 2004). In vitro binding assays show that PiSLF in Petunia inflata physically interacts with S-RNases, although this interaction is stronger with nonself SRNases than with self S-RNases (Hua and Kao, 2006).…”

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confidence: 99%

NaStEP: A Proteinase Inhibitor Essential to Self-Incompatibility and a Positive Regulator of HT-B Stability inNicotiana alataPollen Tubes

et al. 2012

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In Solanaceae, the self-incompatibility S-RNase and S-locus F-box interactions define self-pollen recognition and rejection in an S-specific manner. This interaction triggers a cascade of events involving other gene products unlinked to the S-locus that are crucial to the self-incompatibility response. To date, two essential pistil-modifier genes, 120K and High Top-Band (HT-B), have been identified in Nicotiana species. However, biochemistry and genetics indicate that additional modifier genes are required. We recently reported a Kunitz-type proteinase inhibitor, named NaStEP (for Nicotiana alata Stigma-Expressed Protein), that is highly expressed in the stigmas of self-incompatible Nicotiana species. Here, we report the proteinase inhibitor activity of NaStEP. NaStEP is taken up by both compatible and incompatible pollen tubes, but its suppression in Nicotiana spp. transgenic plants disrupts S-specific pollen rejection; therefore, NaStEP is a novel pistil-modifier gene. Furthermore, HT-B levels within the pollen tubes are reduced when NaStEP-suppressed pistils are pollinated with either compatible or incompatible pollen. In wild-type self-incompatible N. alata, in contrast, HT-B degradation occurs preferentially in compatible pollinations. Taken together, these data show that the presence of NaStEP is required for the stability of HT-B inside pollen tubes during the rejection response, but the underlying mechanism is currently unknown.

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“…In Brassica species, highly divergent regions from 30 to 56 kbp are present in the S -locus region, and this region is fl anked by sequences with high similarity (Fukai et al 2003 ;Shiba et al 2003 ). This polymorphic region is also observed in Prunus Rahman et al 2007a ) and Malus (Ushijima et al 2001 ;Entani et al 2003 ;Sassa et al 2007 ). In these plants, male-and female-determinant genes involved in SI are located within these polymorphic regions in the S -locus region.…”

Section: S -Locus Of I Trifi Damentioning

confidence: 98%

“…In S -RNase-based GSI, S -RNases are abundantly expressed in the transmitting tract of the style, and they directly inhibit the growth of self-pollen tubes (Lee et al 1994 ). The pollen determinant genes, SFB / SLF and related genes, are expressed in developing pollen grains and pollen tubes (Ushijima et al 2003 ;Entani et al 2003 ;Sijacic et al 2004 ;Sassa et al 2007Sassa et al , 2010Meng et al 2010 ;Kakui et al 2011 ), and they recognize self or non-self S -RNases (Meng et al 2010 ;Sassa et al 2010 ). In Papaver plants, the female S -gene product, PrsS, is an extracellular signaling molecule that acts as a ligand to the self male S -gene product, PrpS, a transmembrane ion-channel/receptor.…”

Section: Genes Located At or Near The S -Locus Of I Trifi Damentioning

confidence: 99%

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

Tsuchiya

2014

Sexual Reproduction in Animals and Plants

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Diploid Ipomoea trifi da (Convolvulaceae) is a close relative of the cultivated hexaploid Ipomoea batatas , the cultivated sweet potato. These plants have sporophytic self-incompatibility that is regulated by a single multiallelic locus, designated as the S -locus. Genetic analyses of I. trifi da plants collected from Central America identifi ed about 50 different S -haplotypes with a linear dominance hierarchy having some codominance relationships. A linkage map of DNA markers around the S -locus indicated that the S -locus is delimited to 0.23 cM. Within the S -locus genomic region, a hypervariable genomic region of 35-95 kbp was identifi ed, and we designated this region SDR ( S -locus-specifi c divergent region). Of the several genes located within the SDR, one anther-specifi c gene, AB2 , and three stigma-specifi c genes, SE1 , SE2 , and SEA , are candidate S -genes that may encode male and female S -determinants of self-incompatibility.Keywords Ipomoea trifi da • Self-incompatibility • Sweet potato IntroductionSelf-incompatibility (SI) is a genetic mechanism to prevent self-fertilization (and thus encourage outcrossing) in angiosperms. In self-incompatible plants, when a pollen grain is recognized as the same type as self, some stage of pollen germination, pollen tube elongation, ovule fertilization, or embryo development is halted, and no seeds are produced. SI is classifi ed into several groups: homomorphic SI, heteromorphic SI, cryptic SI (CSI), and late-acting SI. Heteromorphic SI is classifi ed into two groups: distyly is determined by a single locus, which has two alleles, and tristyly is determined by two loci, each with two alleles. Heteromorphic SI is sporophytic, in that both alleles in the male plant determine the SI response in the pollen. CSI exists in a limited number of taxa (for example, there is evidence for CSI in Silene vulgaris in the Caryophyllaceae; Glaettli 2004 ). In this mechanism, the simultaneous presence of cross-and self-pollen on the same stigma results in higher seed set from cross-pollen (Bateman 1956 ). Late-acting SI is also termed ovarian SI. In this mechanism, self-pollen germinates and reaches the ovules, but no fruit is set (Seavey and Bawa 1986 ;Sage et al. 1994 ).Homomorphic SI is classifi ed into sporophytic SI (SSI) and gametophytic SI (GSI) . The SSI system is found in species of several plant families, such as the Brassicaceae, Asteraceae, Malvaceae, Betulaceae, Sterculiaceae, Polemoniaceae, and Convolvulaceae (de Nettancourt 2001 ; Allen and Hiscock 2008 ). In the plants in these families, SI is genetically regulated by a single multi-allelic locus, the S -locus . Within the S -locus, a pair of genes (named S -genes ), one encoding the male-determinant molecules and the other the female-determinant molecules, is localized. These genes are essential for the SI reaction, because these gene products contribute to self/non-self recognition. The sets of S -genes are tightly linked at the S -locus, and the S -locus (also called S -haplotype : Nasrallah and Na...

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Comparative analysis of the self‐incompatibility (S‐) locus region of Prunus mume: identification of a pollen‐expressed F‐box gene with allelic diversity

Cited by 300 publications

References 52 publications

Supergenes and their role in evolution

Supergenes and their role in evolution

NaStEP: A Proteinase Inhibitor Essential to Self-Incompatibility and a Positive Regulator of HT-B Stability inNicotiana alataPollen Tubes

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

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