Comparative Analysis of the Spindle of Fresh In Vivo-Matured Human Oocytes Through Polarized Light and Confocal Microscopy: A Pilot Study

Dib, Luciana Azôr; Broi, Michele Gomes Da; Navarro, Paula Andrea

doi:10.1177/1933719113519174

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…This comparison showed that the non-visualization of the spindle is associated with meiosis abnormalities. This was confirmed by another study, but using oocytes matured in vitro [ 16 ]. This technique could be considered in clinical practice but is costly and requires significant experience.…”

Section: Other Criteria To Assess Oocyte Qualitysupporting

confidence: 71%

Methods for Assessing Oocyte Quality: A Review of Literature

et al. 2022

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The rate of infertility continues to rise in the world for several reasons, including the age of conception and current lifestyle. We list in this paper potential non-invasive and invasive techniques to assess oocyte quality. We searched the database PubMed using the terms “oocytes AND quality AND evaluation”. In the first part, we study the morphological criteria, compartment by compartment, to then focus in a second part on more objective techniques such as genetics, molecular, apoptosis, or human follicular fluid that contain biologically active molecules. The main criteria used to assess oocyte quality are morphological; however, several other techniques have been studied in women to improve oocyte quality assessment, but most of them are invasive and not usable in routine.

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Section: Other Criteria To Assess Oocyte Qualitysupporting

confidence: 71%

Methods for Assessing Oocyte Quality: A Review of Literature

et al. 2022

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“…De Vos et al . in a randomized sibling‐oocyte study reported that fertilization rate, embryo formation and clinical pregnancy were not different in IMSI and ICSI in male factor patients (De Vos et al ., ). Daniel Luna et al .…”

Section: High Magnification Imaging Systemmentioning

confidence: 97%

“…A notable matter in ICSI by conventional microscopy is damaging the spindle and chromosomes when inserting the ICSI needle into the ooplasm due to unclearing the MS. The location of the first PB is not being a reliable predictor of the MS position, because the MS is not always beneath the first PB (Montag et al, 2011;Dib et al, 2014). Rienzi and associates reported a reduced fertilization rate in oocytes with displacement of the first PB with regard to MS of more than 90° (Rienzi et al, 2003).…”

Section: Spindle Presence/position and Art Outcomesmentioning

confidence: 99%

Noninvasive imaging systems for gametes and embryo selection in IVF programs: a review

Omidi

Faramarzi

Agha‐Rahimi

et al. 2017

Journal of Microscopy

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Optimizing the efficiency of the in vitro fertilization procedure by improving pregnancy rates and reducing the risks of multiple pregnancies simultaneously are the primary goals of the current assisted reproductive technology program. With the move to single embryo transfers, the need for more cost-effective and noninvasive methods for embryo selection prior to transfer is paramount. These aims require advancement in a more acquire gametes/embryo testing and selection procedures using high-tech devices. Therefore, the aim of the present review is to evaluate the efficacy of noninvasive imaging systems in the current literatures, focusing on the potential clinical application in infertile patients undergoing assisted reproductive technology treatments. In this regards, three advanced imaging systems of motile sperm organelle morphology examination, polarization microscopy and time-lapse monitoring for the best selection of the gametes and preimplantation embryos are introduced in full.

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