Comparative analysis of two 14-3-3 homologues and their expression pattern in the root-knot nematode Meloidogyne incognita

Jaubert, Stéphanie; Laffaire, Jean Baptiste; Ledger, T. N.; Escoubas, Pierre; Amri, Ez-Zoubir; Abad, Pierre; Rosso, Marie‐Noëlle

doi:10.1016/j.ijpara.2004.02.008

Cited by 42 publications

(30 citation statements)

References 37 publications

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“…Among the 10 most abundant proteins in our data ( Table S1 ), 14-3-3b protein and calreticulin were previously shown to be produced and secreted by the SvG of M. incognita [25],[26].…”

Section: Resultsmentioning

confidence: 71%

Direct Identification of the Meloidogyne incognita Secretome Reveals Proteins with Host Cell Reprogramming Potential

et al. 2008

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The root knot nematode, Meloidogyne incognita, is an obligate parasite that causes significant damage to a broad range of host plants. Infection is associated with secretion of proteins surrounded by proliferating cells. Many parasites are known to secrete effectors that interfere with plant innate immunity, enabling infection to occur; they can also release pathogen-associated molecular patterns (PAMPs, e.g., flagellin) that trigger basal immunity through the nematode stylet into the plant cell. This leads to suppression of innate immunity and reprogramming of plant cells to form a feeding structure containing multinucleate giant cells. Effectors have generally been discovered using genetics or bioinformatics, but M. incognita is non-sexual and its genome sequence has not yet been reported. To partially overcome these limitations, we have used mass spectrometry to directly identify 486 proteins secreted by M. incognita. These proteins contain at least segmental sequence identity to those found in our 3 reference databases (published nematode proteins; unpublished M. incognita ESTs; published plant proteins). Several secreted proteins are homologous to plant proteins, which they may mimic, and they contain domains that suggest known effector functions (e.g., regulating the plant cell cycle or growth). Others have regulatory domains that could reprogram cells. Using in situ hybridization we observed that most secreted proteins were produced by the subventral glands, but we found that phasmids also secreted proteins. We annotated the functions of the secreted proteins and classified them according to roles they may play in the development of root knot disease. Our results show that parasite secretomes can be partially characterized without cognate genomic DNA sequence. We observed that the M. incognita secretome overlaps the reported secretome of mammalian parasitic nematodes (e.g., Brugia malayi), suggesting a common parasitic behavior and a possible conservation of function between metazoan parasites of plants and animals.

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“…Among the 10 most abundant proteins in our data ( Table S1 ), 14-3-3b protein and calreticulin were previously shown to be produced and secreted by the SvG of M. incognita [25],[26].…”

Section: Resultsmentioning

confidence: 71%

Direct Identification of the Meloidogyne incognita Secretome Reveals Proteins with Host Cell Reprogramming Potential

et al. 2008

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“…The identification of effectors known to act intracellularly (Jaubert et al, 2004) and the recent demonstration of physical interactions between an RKN secretion and plant transcription factors (Huang et al, 2006a) support the activity of RKN secretions in the plant cell cytoplasm. Determining whether stylet secretions are injected in the cytoplasm and their role in the induction of giant cell formation constitutes a major challenge because of the low amount of such secretions in planta.…”

Section: Article In Pressmentioning

confidence: 85%

“…RKNs have been shown to produce auxin conjugates and cytokinins that may interfere with the hormone balance of the plant cell (de Meutter et al, 2003(de Meutter et al, , 2005, but it remains unclear whether these nematode products play a role in planta. Interestingly, a 14-3-3 isoform z produced in the esophageal glands of infective juveniles has been identified in purified stylet secretions (Jaubert et al, 2004). In eukaryotic cells, 14-3-3 proteins play a crucial role in modulating the signaling events involved in responses to environmental stimuli, progress through the cell cycle and programmed cell death (Takihara et al, 2000).…”

Section: Article In Pressmentioning

confidence: 99%

Root-knot nematodes manipulate plant cell functions during a compatible interaction

Caillaud

Dubreuil

Quentin

et al. 2008

Journal of Plant Physiology

243

177

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“…14-3-3b protein expressed in the dorsal gland of infective second-stage juveniles (J2) has been identified as being present in the secretome of M. Incognita . This gene may involve in guide protein-protein interactions and has essential roles in hormonal signal transduction processes in plants[55].…”

Section: Resultsmentioning

confidence: 99%

Exploring the Host Parasitism of the Migratory Plant-Parasitic Nematode Ditylenchus destuctor by Expressed Sequence Tags Analysis

et al. 2013

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The potato rot nematode, Ditylenchus destructor, is a very destructive nematode pest on many agriculturally important crops worldwide, but the molecular characterization of its parasitism of plant has been limited. The effectors involved in nematode parasitism of plant for several sedentary endo-parasitic nematodes such as Heterodera glycines, Globodera rostochiensis and Meloidogyne incognita have been identified and extensively studied over the past two decades. Ditylenchus destructor, as a migratory plant parasitic nematode, has different feeding behavior, life cycle and host response. Comparing the transcriptome and parasitome among different types of plant-parasitic nematodes is the way to understand more fully the parasitic mechanism of plant nematodes. We undertook the approach of sequencing expressed sequence tags (ESTs) derived from a mixed stage cDNA library of D. destructor. This is the first study of D. destructor ESTs. A total of 9800 ESTs were grouped into 5008 clusters including 3606 singletons and 1402 multi-member contigs, representing a catalog of D. destructor genes. Implementing a bioinformatics' workflow, we found 1391 clusters have no match in the available gene database; 31 clusters only have similarities to genes identified from D. africanus, the most closely related species to D. destructor; 1991 clusters were annotated using Gene Ontology (GO); 1550 clusters were assigned enzyme commission (EC) numbers; and 1211 clusters were mapped to 181 KEGG biochemical pathways. 22 ESTs had similarities to reported nematode effectors. Interestedly, most of the effectors identified in this study are involved in host cell wall degradation or modification, such as 1,4-beta-glucanse, 1,3-beta-glucanse, pectate lyase, chitinases and expansin, or host defense suppression such as calreticulin, annexin and venom allergen-like protein. This result implies that the migratory plant-parasitic nematode D. destructor secrets similar effectors to those of sedentary plant nematodes. Finally we further characterized the two D. destructor expansin proteins.

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Comparative analysis of two 14-3-3 homologues and their expression pattern in the root-knot nematode Meloidogyne incognita

Cited by 42 publications

References 37 publications

Direct Identification of the Meloidogyne incognita Secretome Reveals Proteins with Host Cell Reprogramming Potential

Direct Identification of the Meloidogyne incognita Secretome Reveals Proteins with Host Cell Reprogramming Potential

Root-knot nematodes manipulate plant cell functions during a compatible interaction

Exploring the Host Parasitism of the Migratory Plant-Parasitic Nematode Ditylenchus destuctor by Expressed Sequence Tags Analysis

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