Comparative analysis of two different subunits of antigen B from Echinococcus granulosus: gene sequences, expression in Escherichia coli and serological evaluation

Rott, Marilise Brittes; Fernández, Verónica; Farias, Sandra Estrazulas; Ceni, Janine Marta; Ferreira, Henrique Bunselmeyer; Haag, Karen Luisa; Zaha, Arnaldo

doi:10.1016/s0001-706x(00)00069-3

Cited by 65 publications

(78 citation statements)

References 25 publications

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“…The major drawback of these tests is the lack of species specificity, as cross-reactions between AE and CE and many other helminthic infections are commonly observed (cestodes, 89%; nematodes, 39%; trematodes; 30%) [5,20,[37][38][39][40]. Assays based on a recombinant subunit of AgB, denoted rAgB8/2, showed sensitivities of 45-93% and specificities of 86-99% with the same cross-reactions as mentioned above [20,41,42].…”

Section: Discussionmentioning

confidence: 89%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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PURPOSE: Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS: The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS: According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS: Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. Abstract Purpose Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. Methods The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. Results According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and [99.9% for the diagnosis of CE and AE, respectively. Antigens derived from...

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Section: Discussionmentioning

confidence: 89%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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“…Considerable efforts have recently been undertaken to develop recombinant proteins with potential usefulness in serodiagnosis. Thus, several recombinant antigens have been expressed and serologically evaluated, including the two different AgB subunits EgAgB1 and EgAgB2, 13,14 and recombinant Ag5, 15 recEpC1 16 and recEgCMDH (cytosolic malate dehydrogenase homologue). 17 Most of these studies preliminarily documented a high diagnostic performance of the recombinant antigens.…”

Section: Introductionmentioning

confidence: 99%

“…17 Most of these studies preliminarily documented a high diagnostic performance of the recombinant antigens. 9,[14][15][16]18,19 Alternatively, synthetic peptides including p176, 20,21 derived from the antigen B sequence, and the 89-122, which mimics an epitope of Ag5, 22 also provided interesting findings. Unfortunately, these recombinant antigens and synthetic peptides were not (or only poorly) investigated for their prognostic value in the serological follow-up of CE.…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular characterization of recombinant Echinococcus granulosus P29 protein (recP29) and its assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients

Nouir

Gianinazzi

Gorcii

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

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SummaryWe synthesized recombinant Echinococcus granulosus protoscolex recP29 antigen to be preliminarily assessed by ELISA and immunoblotting. RecP29-serology was carried out on 54 young patients with cystic echinococcosis (CE). Patients were classified into either cured (CCE) (n = 40) or non-cured (NCCE) (n = 14) CE patients. RecP29 ELISA showed a gradual decrease of antibody concentrations in all CCE cases that were initially (before treatment) seropositive to this antigen (25 out of 40) or that seroconverted following treatment. A complete seronegativity was reached within 3 years post-surgery in all of these cases. Conventional HCF ELISA yielded seronegativity in only 10% of initially recP29-seropositive CCE patients (P = 0.086). Likewise, recP29 immunoblotting yielded seronegativity in 93% of 29 out of 40 initially recP29-immunoblot-positive CCE patients after 3 years follow-up, compared with 72% in the HCF immunoblotting (P = 0.060). Eleven out of 14 NCCE patients were initially positive by recP29 ELISA, and 10 out of these maintained a marked anti-recP29 antibody reactivity until the endpoint of the follow-up period. All 14 NCCE cases were initially seropositive by recP29 immunoblotting, and 13 cases remained seropositive until the end of the study. Thus, recombinant P29 protein appears prognostically useful for monitoring those post-surgical CE cases with an initial seropositivity to this marker.

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“…Accordingly, AgB has received much attention due to its high immunogenicity and specificity [8].Antigen B is encoded by a multigene family-EgAgB8/1, EgAgB8/2, EgAgB8/3, and EgAgB8/4, each of which codes for one subunit of 8 kDa [9,10]. E.granulosus enhances both humoral and cellular responses in its intermediate host.…”

Section: Introductionmentioning

confidence: 99%

“…Comparison of execratory/ secretory protiens from E. granulosus hydatid fluid from several host showed some different patterns . The analysis of sequences related to AgB showed variation between parasites with different hosts and geographical origin [9].…”

Section: Introductionmentioning

confidence: 99%

Efficiency Evaluation of Immunization Protocol with Echinococcus Granulosus Antigen B (Agb)

Mustafa¹,

Shani²

2017

Cihan Univ.-Erbil Sci. J.

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Antigen B (AgB), the major antigens of Echinococcus granulosus, is a polymeric thermostable lipoprotein that constitutes as much as 10% of hydatid fluid.Antibody and cytokine response were both studied in rabbits immunized with Echinococcus granulosus antigen B (AgB). This study aimed to assess the effect of antigen B (AgB) on immune response for this purpose hydatid cysts were collected from liver of infected sheep from slaughter house in Basra . AgB was purified from hydatid fluid by anion exchange chromatography.For evaluation of AgB efficiency in immunization , 15 rabbits were enrolled in the study ,10 of them were immunized subcutaneously with AgB using freund's complete adjuvant after two weeks three booster injections were repeated intramuscularly according to immunization schedule using freund's incomplete adjuvant and 5 rabbits were treated as a control group. After six weeks , blood were collected from rabbits and the levels of IgG , IFN-γ and IL-10 were measured by ELISA test.Means of IgG of immunized rabbits were significantly (P < 0.05) elevated (45.9) ng/ml in comparison with control rabbits (16.6) ng/ml. Cytokines levels of Th1 (IFN-γ) and Th2 (IL-10) were recorded a significant differences (P < 0.05) between immunized and control groups , the mean concentrations of IFN-γ in immunized rabbits (28.6 pg/ml) was lower than those of control group (83.6 pg/ml) while IL-10 mean concentrations in immunized rabbits (60.1) pg/ml was higher than those of control group (18.8) pg/ml .The results indicated that AgB has the ability to modulate immune response toward Th2 bias in immunized rabbits as there was significant elevation in total IgG, IL-10 and significant decreasing IFN-γ concentration.

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Comparative analysis of two different subunits of antigen B from Echinococcus granulosus: gene sequences, expression in Escherichia coli and serological evaluation

Cited by 65 publications

References 25 publications

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Isolation and molecular characterization of recombinant Echinococcus granulosus P29 protein (recP29) and its assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients

Efficiency Evaluation of Immunization Protocol with Echinococcus Granulosus Antigen B (Agb)

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