Comparative aroma and nutrients profiling in six edible versus nonedible cruciferous vegetables using MS based metabolomics

Baky, Mostafa H.; Shamma, Samir; Xiao, Jianbo; Farag, Mohamed A.

doi:10.1016/j.foodchem.2022.132374

Cited by 34 publications

(25 citation statements)

References 37 publications

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“…Finally, the NIST mass spectral library and an in-house mass spectral database established using authentic standards were used together for metabolite identification ( Yan et al, 2021 ). Their retention indices were compared with n -alkanes ranging from C7-C36 for identification of volatile and non-volatile silylated metabolites according to previous reports ( Table S1 ) ( Baky et al, 2022 ). Based on the normalized metabolomic data, the heat maps of detected metabolites was drawn using TBtools (v1.082, Guangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence

et al. 2022

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“…Orbitrap-MS can obtain high-resolution data for accurate exact mass and elemental composition assignment, while TQ-MS is often used for quantitative analysis due to its high sensitivity. Several studies have demonstrated the power of using these technologies for volatile and non-volatile metabolite analysis [ 34 , 38 ]. For example, the nutritional components of six cruciferous vegetables were investigated by HS-SPME/GC-MS non-targeted analysis, and a total of 55 nutritional metabolites and 190 volatiles were detected [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have demonstrated the power of using these technologies for volatile and non-volatile metabolite analysis [ 34 , 38 ]. For example, the nutritional components of six cruciferous vegetables were investigated by HS-SPME/GC-MS non-targeted analysis, and a total of 55 nutritional metabolites and 190 volatiles were detected [ 38 ]. In a separate study, a total of 47 nutritional components in the flavedo, albedo and pulp of pummelo fruits were determined by UHPLC-TQ-MS targeted analysis.…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Guo

Lai

et al. 2022

Metabolites

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Coconut is a tropical fruit whose flesh has high flavor quality and nutritional value; however, the differences between coconut varieties are still unclear. Here, volatiles and non-volatiles were profiled at three ripening stages by HS-SPME/GC-MS and UHPLC-MS/MS in two coconut varieties (Hainan Tall, HT and Green Dwarf, GD). Four metabolite classes of volatiles were associated with good aroma including hydrocarbons, benzenoids, alcohols and esters, and these volatiles were generally higher in GD, especially at 7 and 9 months of coconut growth. Pathway-based metabolomics revealed that flavonols and their derivatives were significantly enriched in HT, and some of these metabolites were key determinants of HT flesh bitterness, including kaempferol 7-O-glucoside, a known bitter metabolite. Despite the overall accumulation of amino acids, including L-alanine, L-serine and L-methionine in GD, comparative metabolomics revealed that HT flesh provides a higher content of vitamins than GD. This study sheds light on the metabolic pathways and key metabolites differentiating the flesh flavor quality and nutritional value among coconut varieties, and reveals the possible mechanisms of flavor formation and regulation in coconut fruits.

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“…On the other hand, BITC content has been scarcely reported in vegetables. However, it is one of the predominant isothiocyanates in cauliflower and radish (4.5 and 2.2%, respectively) among the total myrosinase-mediated glucosinolate hydrolysis products [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Isothiocyanate-Rich Extracts from Cauliflower (Brassica oleracea Var. Botrytis) and Radish (Raphanus sativus) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells

Cuellar-Núñez

Luzardo-Ocampo

Lee-Martínez

et al. 2022

IJERPH

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Cruciferous vegetables such as cauliflower and radish contain isothiocyanates exhibiting chemoprotective effects in vitro and in vivo. This research aimed to assess the impact of cauliflower (CIE) and radish (RIE) isothiocyanate extracts on the metabolic activity, intracellular reactive oxygen species (ROS), and LDH production of selected human colorectal adenocarcinoma cells (HCT116 and HT-29 for early and late colon cancer development, respectively). Non-cancerous colon cells (CCD-33Co) were used as a cytotoxicity control. The CIE samples displayed the highest allyl isothiocyanate (AITC: 12.55 µg/g) contents, whereas RIE was the most abundant in benzyl isothiocyanate (BITC: 15.35 µg/g). Both extracts effectively inhibited HCT116 and HT-29 metabolic activity, but the CIE impact was higher than that of RIE on HCT116 (IC50: 0.56 mg/mL). Assays using the half-inhibitory concentrations (IC50) of all treatments, including AITC and BITC, displayed increased (p < 0.05) LDH (absorbance: 0.25–0.40 nm) and ROS release (1190–1697 relative fluorescence units) in both cell lines. BITC showed the highest in silico binding affinity with all the tested colorectal cancer molecular markers (NF-kB, β-catenin, and NRF2-NFE2). The theoretical evaluation of AITC and BITC bioavailability showed high values for both compounds. The results indicate that CIE and RIE extracts display chemopreventive effects in vitro, but additional experiments are needed to validate their effects.

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Comparative aroma and nutrients profiling in six edible versus nonedible cruciferous vegetables using MS based metabolomics

Cited by 34 publications

References 37 publications

Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence

Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Isothiocyanate-Rich Extracts from Cauliflower (Brassica oleracea Var. Botrytis) and Radish (Raphanus sativus) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells

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