Comparative assessment of structural and biological properties of biomimetically coated hydroxyapatite on alumina (α‐Al₂O₃) and titanium (Ti‐6Al‐4V) alloy substrates

Abstract

Previous reports have shown the use of hydroxyapatite (HAp) and related calcium phosphate coatings on metal and nonmetal substrates for preparing tissue-engineering scaffolds, especially for osteogenic differentiation. These studies have revealed that the structural properties of coated substrates are dependent significantly on the method and conditions used for coating and also whether the substrates had been modified prior to the coating. In this article, we have done a comparative evaluation of the structural features of the HAp coatings, prepared by using simulated body fluid (SBF) at 25 degrees C for various time periods, on a nonporous metal substrate titanium-aluminium-vanadium (Ti-6Al-4V) alloy and a bioinert ceramic substrate alpha-alumina (alpha-Al(2)O(3)), with and without their prior treatment with the globular protein bovine serum albumin (BSA). Our analysis of these substrates by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectrometry showed significant and consistent differences in the quantitative and qualitative properties of the coatings. Interestingly, the bioactivity of these substrates in terms of supporting in vitro cell adhesion and spreading, and in vivo effects of implanted substrates, showed a predictable pattern, thus indicating that some coated substrates prepared under our conditions could be more suitable for biological/biomedical applications.

Bioinspired and Biomimetic Functional Hybrids as Tools for Regeneration of Orthopedic Interfaces

“…In our method, the metal /ceramic substrates were incubated in simulated body fluid (SBF) at 25°C for different time points with prior treatment with globular protein BSA (bovine serum albumin) [Chakraborty et al, 2009;Kapoor et al, 2010]. This process leads to the formation of HAp coating exhibiting bone like apatite growth on the surface.…”

“…Mg-Zn-Zr, HA-PEEK poly (aryl-etherether-ketone), Polyphospha zenes, BG-COL-HYA-PS (glass-collagen hyaluronic acid-phosphatidylserine) Yeung et al,2007;Aksakal et al, 2008;Seligson et al,1997;Marti 2000Aksakal et al, 2008Chakraborty et al, 2009;Yeung et al,2007;Banerjee et al, 2004;Banerjee et al, 2006;Niinomi 2003;Ning et al, 2010;Seligson et al,1997Kapoor et al, 2010Christel et al, 1988;Gorustovich et al, 2010;Yuan et al, 2001Andersson et al, 2004Reis et al, 2010;Oral et al, 2007;Butler et al, 2001;Athanasiou et al, 1998;Smith et al,2007;Geary et al, 2008;Shalumon et al, 2009;Jayabalan et al, 2001Ye et al,2010Kurtz et al, 2007;Sethuraman et al, 2010;Xu et al, 2010 Since there is no material available that can per se become a bone substitute, several modifications on the original material are required to make it biocompatible. The aim to do these modifications is that the new material should be nontoxic and biologically inert but yet it should show orthopedic bioactivity and its production should be cost effective.…”

“…The design and manufacturing of orthopedic bimimetic implants is meant to replace parts of the hard and weight-bearing bones [Rigo et al, 2004;Chakraborty et al, 2009;Kapoor et al, 2010;Nair et al, 2009;Geary et al, 2008] and replacing soft orthopedic tissue [Balasundaram & Webster, 2007]. Further improvements in the implant's properties, which could include the incorporation of bioactive molecules such as long chain proteins or short peptide sequences [Chakraborty et al, 2009;Kapoor et al, 2010;Balasundaram & Webster, 2007;Keselowsky et al, 2005;Le et al, 2006;Stevens et al, 2008;Sato &Webster, 2004], are necessary to make them more acceptable by the cells at the site and thus ensure their long term success. This is an ongoing process and several new developments have taken in this field in recent years.…”

Avaliação histológica e histomorfométrica da energia superficial e molhabilidade em implantes de titânio grau IV e grau V: estudo experimental em coelhos

“…Mas o uso de biomateriais diversos não se faz presente só em implantes dentários, mas em substitutos ósseos em casos de enxertos, com o uso de cerâmicas, polímeros e minerais naturais (ANANTH et al, 2015;KAPOOR et al, 2010).…”

“…Modificações em sua morfologia e superfície já são utilizadas para influenciar diretamente as respostas teciduais, alguns tipos de superfícies de metal e não metais têm sido utilizados com ou sem revestimentos, com a finalidade de aumentar o embricamento mecânico entre superfície do implante e tecido ósseo, aperfeiçoando a estabilidade primária, dissipação de forças e resistência (ANANTH et al, 2015;KAPOOR et al, 2010;MISCH, 2008).…”