Comparative characterization of two galectins excreted-secreted from intestine-dwelling parasitic versus free-living females of the soil-transmitted nematode Strongyloides

Ditgen, Dana; Anandarajah, Emmanuela M.; Reinhardt, Anika; Younis, Abuelhassan Elshazly; Witt, Susanne; Hansmann, Jan; Lorenz, Eva; García-Hernández, Marisela; Paclik, Daniela; Soblik, Hanns; Jolodar, Abbas; Seeberger, Peter H.; Liebau, Eva; Brattig, Norbert W.

doi:10.1016/j.molbiopara.2018.08.008

Cited by 11 publications

(12 citation statements)

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“…The S. ratti galectins promoted release of the type 2 cytokines thymic stromal lymphopoietin and IL-22 from mucosal cells, supporting their immunoregulatory functions. They also stimulated cell migration in a dose-dependent manner [96]. Further studies to identify the biological functions of other salient ES proteins in parasitic females and iL3 stages of Strongyloides spp.…”

Section: The Proteomesmentioning

confidence: 93%

“…These constitute the excretory and secretory proteins (the ES proteome), which modulate the immune systems of their vertebrate hosts, allowing the parasitic stages of the worms to survive longer [94]. Excretory and secretory proteins are likely crucial in establishing and maintaining parasitism by nematodes at all stages of infection, including recognition of the host, tissue penetration, somatic migration, and immune evasion throughout these processes [94][95][96][97]. These essential functions make proteins of the ES secretome promising targets for drugs or vaccines.…”

Section: The Proteomesmentioning

confidence: 99%

“…Despite the efforts cited above, the functions and biological significance of most of the ES proteins from S. ratti, S. stercoralis, and S. venezuelensis remain largely unknown. Notable exceptions are two galectins, Sr-GAL-1 and Sr-GAL-3, excreted-secreted by S. ratti [96]. Galectins are a large group of β-galactoside-binding proteins which are involved in several biological processes especially in host-parasite interactions.…”

Section: The Proteomesmentioning

confidence: 99%

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Advances in the Molecular and Cellular Biology of Strongyloides spp.

Jaleta

Lok

2019

Curr Trop Med Rep

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Purpose of Review This paper constitutes an update of recent studies on the general biology, molecular genetics, and cellular biology of Strongyloides spp. and related parasitic nematodes. Recent Findings Increasingly, human strongyloidiasis is considered the most neglected of neglected tropical diseases. Despite this, the last 5 years has seen remarkable advances in the molecular biology of Strongyloides spp. Genome sequences for S. stercoralis, S. ratti, S. venezuelensis, S. papillosus, and the related parasite Parastrongyloides trichosuri were created, annotated, and analyzed. These genomic resources, along with a practical transgenesis platform for Strongyloides spp., aided a major achievement, the advent of targeted mutagenesis via CRISPR/Cas9 in S. stercoralis and S. ratti. The genome sequences have also enabled significant molecular epidemiologic and phylogenetic findings on human strongyloidiasis, including the first genetic evidence of zoonotic transmission of S. stercoralis between dogs and humans. Studies of molecular signaling pathways identified the nuclear receptor Ss-DAF-12 as one that can be manipulated in the parasite by exogenous application of its steroid ligands. The chemotherapeutic implications of this were unscored by a study in which a Ss-DAF-12 ligand suppressed autoinfection by S. stercoralis in a new murine model of human strongyloidiasis. Summary Seminal advances in genomics of Strongyloides spp. have transformed research into strongyloidiasis, facilitating fundamental phylogenetic and epidemiologic studies and aiding the deployment of CRISPR/Cas9 gene disruption and editing as functional genomic tools in Strongyloides spp. Studies of Ss-DAF-12 signaling in S. stercoralis demonstrated the potential of this pathway as a novel chemotherapeutic target in parasitic nematodes.

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Section: The Proteomesmentioning

confidence: 93%

Section: The Proteomesmentioning

confidence: 99%

Section: The Proteomesmentioning

confidence: 99%

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Advances in the Molecular and Cellular Biology of Strongyloides spp.

Jaleta

Lok

2019

Curr Trop Med Rep

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“…A galectina já foi identificada em larvas infectantes de S. stercoralis após a digestão prolongada com tripsina (Marcilla et al, 2010;Ditgen et al, 2018). Os genes codificantes para as proteínas em questão foram identificados em outras espécies de nematódeos parasitas incluindo (Newton et al, 1997;Greenhalgh et al, 2000;Kiel et al, 2007;Jeong et al, 2013;Gonzáles-Miguel et al, 2015;Shi et al, 2018).…”

Section: Western-blotting 2dunclassified

“…Neste estudo, a galectina foi identificada principalmente na fração de membrana (TM) utilizando gel 1D e 2D. Rodpai et al ( e 2017 A partir dos resultados imunoproteômicos apresentados no presente estudo e com base na importância dada pela literatura Ditgen et al, 2018)…”

Section: Western-blotting 2dunclassified