Comparative chromosome painting map between two Ryukyu spiny rat species, Tokudaia osimensis and Tokudaia tokunoshimensis (Muridae, Rodentia)

Nakamura, Taro; Kuroiwa, Asato; Nishida‐Umehara, Chizuko; Matsubara, Kazumi; Yamada, Fumio; Matsuda, Yoichi

doi:10.1007/s10577-007-1163-9

Cited by 20 publications

(15 citation statements)

References 30 publications

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“…This was not observed in the other two Tokudaia species, where the chromosomal segments recognized by MMU11 and MMU16 corresponded to chromosome 3q in T. osimensis and chromosome 10 in T. tokunoshimensis, respectively (Nakamura et al 2007). The fusion of a pair of autosomes with both the X and Y chromosomes is a rare chromosomal rearrangement in mammals.…”

Section: Fusion Of Autosomes and The Sex Chromosomesmentioning

confidence: 73%

“…The Zoo-FISH analysis was performed using biotin-and Cy3-labeled chromosome-specific painting probes from the laboratory mouse (Cambio Ltd.). FISH was carried out as described by Nakamura et al (2007) with slight modifications. The probes were denatured at 75°C for 10 min and pre-annealed by incubation at 37°C for 1 h. The chromosome slides were hybridized with the biotin-or Cy3-labeled probes at 37°C for 5 days.…”

Section: Dna Probesmentioning

confidence: 99%

“…TTO, T. tokunoshimensis chromosome tokunoshimensis generated by chromosome painting (Nakamura et al 2007): MMU1b/17a/17e, a single segment of MMU14, and the two distinct chromosomal segments of MMU15 occurred in T. muenninki, whereas MMU1b/17a, two distinct chromosomal segments of MMU14, and a single segment of MMU15 occurred in T. osimensis and T. tokunoshimensis. The former segments found in T. muenninki are also conserved in the ancestral karyotype of the genus Apodemus (Matsubara et al 2004), which is the most closely related group to the genus Tokudaia in Murinae (Michaux et al 2002;Sato and Suzuki 2004;Rowe et al 2008), suggesting that the ancestor of genus Tokudaia might have the same segments as T. muenninki, MMU1b/17a/17e, two distinct chromosomal segments of MMU14 and a single segment of MMU15.…”

Section: Ancestral Karyotype Of the Genus Tokudaiamentioning

confidence: 99%

“…R-and Hoechst G-banding patterns are shown in (a, c, e, g, i, k) and (b, d, f, h, j, l), respectively. The scale bar indicates 10 μm species (Arakawa et al 2002;Nakamura et al 2007) and six Apodemus species (Matsubara et al 2004), which suggested that no chromosomal variation had occurred in T. muenninki in these regions.…”

Section: Ancestral Karyotype Of the Genus Tokudaiamentioning

confidence: 99%

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The Y chromosome of the Okinawa spiny rat, Tokudaia muenninki, was rescued through fusion with an autosome

Murata

Yamada

Kawauchi³

et al. 2011

Chromosome Res

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The genus Tokudaia comprises three species, two of which have lost their Y chromosome and have an XO/XO sex chromosome constitution. Although Tokudaia muenninki (Okinawa spiny rat) retains the Y chromosome, both sex chromosomes are unusually large. We conducted a molecular cytogenetic analysis to characterize the sex chromosomes of T. muenninki. Using crossspecies fluorescence in situ hybridization (Zoo-FISH), we found that both short arms of the T. muenninki sex chromosomes were painted by probes from mouse chromosomes 11 and 16. Comparative genomic hybridization analysis was unable to detect sex-specific regions in the sex chromosomes because both sex probes highlighted the large heterochromatic blocks on the Y chromosome as well as five autosomal pairs. We then performed comparative FISH mapping using 29 mouse complementary DNA (cDNA) clones of the 22 X-linked genes and the seven genes linked to mouse chromosome 11 (whose homologue had fused to the sex chromosomes), and FISH mapping using two T. muenninki cDNA clones of the Y-linked genes. This analysis revealed that the ancestral gene order on the long arm of the X chromosome and the centromeric region of the short arm of the Y chromosome were conserved. Whereas six of the mouse chromosome 11 genes were also mapped to Xp and Yp, in addition, one gene, CBX2, was also mapped to Xp, Yp, and chromosome 14 in T. muenninki. CBX2 is the candidate gene for the novel sex determination system in the two other species of Tokudaia, which lack a Y chromosome and SRY gene. Overall, these results indicated that the Y chromosome of T. muenninki avoided a loss event, which occurred in an ancestral lineage of T. osimensis and T. tokunoshimensis, through fusion with an autosome. Despite retaining the Y chromosome, sex determination Chromosome Res (2012) 20:111-125

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Section: Fusion Of Autosomes and The Sex Chromosomesmentioning

confidence: 73%

Section: Dna Probesmentioning

confidence: 99%

Section: Ancestral Karyotype Of the Genus Tokudaiamentioning

confidence: 99%

Section: Ancestral Karyotype Of the Genus Tokudaiamentioning

confidence: 99%

See 2 more Smart Citations

The Y chromosome of the Okinawa spiny rat, Tokudaia muenninki, was rescued through fusion with an autosome

Murata

Yamada

Kawauchi³

et al. 2011

Chromosome Res

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“…However, some species with conserved genomes have been identified. For example, a single chromosomal rearrangement distinguishes Apodemus (Matsubara et al, 2004;Stanyon et al, 2004) and relatively high genome conservation was established for species within Rattus and Tokudaia, representatives of the tribe Rattini (Guilly et al, 1999;Stanyon et al, 1999;Cavagna et al, 2002;Nakamura et al, 2007;Badenhorst et al, 2011).…”

Section: Muridaementioning

confidence: 99%

Chromosomal evolution in Rodentia

et al. 2011

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Rodentia is the most species-rich mammalian order and includes several important laboratory model species. The amount of new information on karyotypic and phylogenetic relations within and among rodent taxa is rapidly increasing, but a synthesis of these data is currently lacking. Here, we have integrated information drawn from conventional banding studies, recent comparative painting investigations and molecular phylogenetic reconstructions of different rodent taxa. This permitted a revision of several ancestral karyotypic reconstructions, and a more accurate depiction of rodent chromosomal evolution.

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Spiny rat SRY lacks a long Q‐rich domain and is not stable in transgenic mice

Ogata

Nishikata

Kitada

et al. 2019

Developmental Dynamics

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Background: Although Tokudaia muenninki has multiple extra copies of the Sry gene on the Y chromosome, loss of function of these sequences is indicated. To examine the Sry gene function for sex determining in T. muenninki, we screened a BAC library and identified a clone (SRY26) containing complete SRY coding and promoter sequences. Results: SRY26 showed high identity to mouse and rat SRY. In an in vitro reporter gene assay, SRY26 was unable to activate testis-specific enhancer of Sox9. Four lines of BAC transgenic mice carrying SRY26 were generated. Although the embryonic gonads of XX transgenic mice displayed sufficient expression levels of SRY26 mRNA, these mice exhibited normal female phenotypes in the external and internal genitalia, and up-regulation of Sox9 was not observed. Expression of the SRY26 protein was confirmed in primate-derived COS7 cells transfected with a SRY26 expression vector. However, the SRY26 protein was not expressed in the gonads of BAC transgenic mice. Conclusions: Overall, these results support a previous study demonstrated a long Q-rich domain plays essential roles in protein stabilization in mice. Therefore, the original aim of this study, to examine the function of the Sry gene of this species, was not achieved by creating TG mice. K E Y W O R D SBAC, neo-Y, transgenic mouse, Y chromosome

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Comparative chromosome painting map between two Ryukyu spiny rat species, Tokudaia osimensis and Tokudaia tokunoshimensis (Muridae, Rodentia)

Abstract: Ryukyu spiny rats (genus Tokudaia) are indigenous species that are confined to three islands of the Nansei Shoto archipelago, Amami-Oshima, Tokunoshima and Okinawa-jima, Japan. Tokudaia tokunoshimensis from Tokunoshima Island and

Cited by 20 publications

References 30 publications

The Y chromosome of the Okinawa spiny rat, Tokudaia muenninki, was rescued through fusion with an autosome

The Y chromosome of the Okinawa spiny rat, Tokudaia muenninki, was rescued through fusion with an autosome

Chromosomal evolution in Rodentia

Spiny rat SRY lacks a long Q‐rich domain and is not stable in transgenic mice

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