Comparative cryopreservation study of trochophore larvae from two species of bivalves: Pacific oyster (Crassostrea gigas) and Blue mussel (Mytilus galloprovincialis)

Exploring the evolution of marine invertebrate cryopreservation – Landmarks, state of the art and future lines of research

“…For C. gigas Grout and McFadzen (1991) reported on a patent application, survivals after 10 days of incubation of 70-90% using 15% (v/v) Me 2 SO. Less successful was the use of 10-15% (v/v) PG (Gwo 1994, Paniagua-Chavez 2011) reporting respectively 38 and 24 % development to D-veliger larvae and Paredes et al 2013 reported 60% development to D-veliger larvae when using 10% (v/v) EG.…”

“…(Naidenko et al 1997, Odintsova et al 2006), antioxidants (Naidenko et al 1998, Odintsova et al 2009) and Bovine Serum Albumin (BSA) (Paredes et al 2012, Paredes et al 2013.…”

Freezing tolerance of sea urchin embryonic cells: Differentiation commitment and cytoskeletal disturbances in culture

“…These and other findings [36,46,47,54] have provided enough successful protocols for cryopreservation of marine invertebrate cells and larvae that will allow researchers to work with embryonic cells from these organisms throughout the year. The development of this field not only has environmental significance, but also offers great potential for marine biotechnology, as this field may clarify the cryoresistance mechanisms of marine organisms at ultra-low temperatures.…”

Cryobanking of aquatic species

“…As part of these programs, sperm collected from individuals used to make family crosses in a breeding run is banked. Methods have also been developed for cryopreserving Pacific oyster oocytes and for larvae of greenshell™ mussels and Pacific oyster (Paredes et al, 2012, 2013; Tervit et al, 2005). These methods have not yet been incorporated in either selective breeding or hatchery production and further research is needed to improve the reliability of these methods for both purposes.…”

“…In Australia, gametes and embryos of the blue mussel, M. galloprovincialis , are banked for out of season commercial production (Xiaoxu Li, SARDI, personal communication) but presently there is no banking for selective breeding (Liu and Li, 2015; Paredes et al, 2013). …”