Comparative cytofluorometric DNA analysis of pleomorphic adenoma and adenoid cystic carcinoma of the salivary glands

The clinical relevance of ploidy and S‐phase fraction determination in salivary gland tumors: A flow cytometric study of 97 cases

“…4,6,9,33,41 Therefore, our data and the accumulated literature agree that the low occurrence of DNA aneuploidy is a general rule among salivary gland tumors. The few exceptions to these observations came from the studies of el-Naggar et al, 31 Eibling et al, 12 Timon et al, 14 and all the published data dealing with ploidy analysis in salivary duct carcinoma.…”

“…Greiner et al 4 reported that 95% of the adenoid cystic carcinomas were diploid, and also suggest that SPF may be more useful than ploidy in predicting disease prognosis. Daniele et al 21 immunohistochemically assessed the proliferating cell nuclear antigen nuclear expression in a series of 32 pleomorphic adenomas and 7 adenoid cystic carcinomas and suggested that the distinct biologic behavior of the two types of tumors could be explained by differences in their proliferative activity, which were higher in the latter, in keeping with the conclusions of Saka et al 33 in their image cytometry study. Using a distinct methodology to estimate cell proliferation, our data do not support this view, because a similar value for the mean SPFs was found in the two types of neoplasms.…”

“…The finding is, in general, in agreement with the majority of previous studies in benign mixed tumors. Saka et al 33 studied a series of 17 mixed tumors by image cytometry and did not find DNA aneuploidy cases. Atula et al 34 reported aneuploid clones in 7 of 119 pleomorphic adenomas evaluated, and Daniele et al 21 mentioned only 1 of 32 similar cases.…”

Adenoid Cystic Carcinoma: Clinical and Molecular Features

“…Analyses of chromosomal ploidy have shown that most classic non-solid ACC show a low degree of aneuploidy, much less than other types of carcinomas, but, ACC with solid histology show increased aneuploidy [51,52]. Studies using CGH and aCGH show a correlation of the degree of genetic alterations (change in DNA copy number, chromosome number, genetic deletion) with the degree of solid growth pattern [39,41,42], consistent with the notion that ACCs with solid histology represent a later stage in tumor progression.…”

Proliferative activity in oral salivary gland tumors: the role of PCNA and AgNOR assessed by a double staining technique

“…Although both tumors originate in the intercalated duct with the same cellular derivation (15) and secretion of proteins of the extracellular matrix (16), they exhibit the characteristic behavior of benign and malignant tumors, respectively, coupled with distinct proliferative activity (17)(18)(19)(20) The objective of this study was to evaluate the role of PCNA and AgNOR in the assessment of salivary gland tumor proliferation using a double staining technique.…”