Comparative Detection of Trypanosomal DNA by Loop-Mediated Isothermal Amplification and PCR from Flinders Technology Associates Cards Spotted with Patient Blood

Loop-mediated isothermal amplification assay for the detection of Ehrlichia canis DNA in blood samples from dogs

“…The sensitivity of the assay, which was established using 10-fold serial dilutions of plasmid DNA containing a fragment of the groESL operon, was 1 copy of plasmid DNA. This sensitivity can be compared to that reported for other LAMP assays developed for Anaplasmataceae (Ma et al 2011, Nakao et al 2010 and other studies have demonstrated the higher sensitivity of the method when compared to PCR (Alhassan et al 2007, Cho et al 2006, Ma et al 2011, Matovu et al 2010.…”

Comparison of nucleic acid sequence-based amplification and loop-mediated isothermal amplification for diagnosis of human African trypanosomiasis

“…Nucleic acids amplifications 2.5.1. LAMP reaction LAMP was carried as described by Njiru et al (2008) and Matovu et al (2010), using the primers designed to target the RIME (Njiru et al, 2008). Briefly, the 25 μL of LAMP reaction mixture contained, 2 μmol each of FIP and BIP, 2 μmol each of F3 and B3, and 0.8 μmol of LB and LF primers, 8 U of Bst DNA polymerase large fragment (New England Biolabs, Inc.), 0.2 mmol/L deoxynucleoside triphosphates, 0.8 mol/L betaine, 20 mmol/L Tris-HCl (pH 8.8), 10 mmol/L KCl, 10 mmol/L (NH 4 )2SO 4 , 8 mmol/L MgSO 4 , 0.1% Tween 20, and 4 μL of the boom extracted template.…”

Specific Detection of Klebsiella variicola and K. oxytoca by Loop-Mediated Isothermal Amplification

“…As a result, this technology has been used widely for molecular detection of several microorganisms, including clinical and plant-associated bacterial [32,[61][62][63][64][65][66][67][68][69][70][71][72][73], fungal [74][75][76], viral [47,[77][78][79][80][81][82][83][84], and parasitic [57,59,[85][86][87][88] pathogens, and is therefore suitable as a rapid field test.…”