Comparative differential proteomic analysis of minimal change disease and focal segmental glomerulosclerosis

Abstract

BackgroundMinimal change disease (MCD) and primary focal segmental glomerulosclerosis (FSGS) are glomerular diseases characterized by nephrotic syndrome. Their diagnosis requires a renal biopsy, but it is an invasive procedure with potential complications. In a small biopsy sample, where only normal glomeruli are observed, FSGS cannot be differentiated from MCD. The correct diagnosis is crucial to an effective treatment, as MCD is normally responsive to steroid therapy, whereas FSGS is usually resistant.The purpose of our study was to discover and validate novel early urinary biomarkers capable to differentiate between MCD and FSGS.MethodsForty-nine patients biopsy-diagnosed of MCD and primary FSGS were randomly subdivided into a training set (10 MCD, 11 FSGS) and a validation set (14 MCD, 14 FSGS). The urinary proteome of the training set was analyzed by two-dimensional differential gel electrophoresis coupled with mass spectrometry. The proteins identified were quantified by enzyme-linked immunosorbent assay in urine samples from the validation set.ResultsUrinary concentration of alpha-1 antitrypsin, transferrin, histatin-3 and 39S ribosomal protein L17 was decreased and calretinin was increased in FSGS compared to MCD. These proteins were used to build a decision tree capable to predict patient’s pathology.ConclusionsThis preliminary study suggests a group of urinary proteins as possible non-invasive biomarkers with potential value in the differential diagnosis of MCD and FSGS. These biomarkers would reduce the number of misdiagnoses, avoiding unnecessary or inadequate treatments.Electronic supplementary materialThe online version of this article (doi:10.1186/s12882-017-0452-6) contains supplementary material, which is available to authorized users.

Chronic kidney disease: a review of proteomic and metabolomic approaches to membranous glomerulonephritis, focal segmental glomerulosclerosis, and IgA nephropathy biomarkers

“…They quantified identified proteins from the training set in the urine samples of the validation set by ELISA method. The most significant alterations included decrement of α-1 antitrypsin, transferrin, 39S ribosomal protein L17, and histatin-3, and increased calretinin in FSGS patients compared to MCD [6]. Among these proteins, the role of α-1 antitrypsin in FSGS pathogenesis might be related to its anti-inflammatory and anti-apoptotic actions, as previously shown in renal injuries [92].…”

Proteomic Analysis of Larval Integument in a Dominant Obese Translucent (Obs) Silkworm Mutant

“…A myofilin protein encoded by gi|226903597| has been reported to be involved in the formation of the thick filaments of insect muscle ( Qiu et al 2005 ). BGIBMGA008477 is a silkworm homologue of the gene encoding 39S ribosomal protein L17, which is known to be involved in the process of translation, and has been reported to be a noninvasive biomarker with potential value in the differential diagnosis of minimal change disease and primary focal segmental glomerulosclerosis ( Pérez et al 2017 ). The protein encoded by BGIBMGA008526 is a protein of unknown function.…”

A systematic review and meta-analysis of integrated traditional Chinese medicine and Western medicine in treating glomerulosclerosis

“…Nevertheless, the high recurrence rate and adverse reactions have greatly limited the outcomes of these strategies. [ 5 , 6 ] Traditional Chinese medicine classifies glomerulosclerosis as “consumptive disease,” “urine turbid” etc, and the herbal preparations have been very effective in mitigating the symptoms. [ 7 , 8 ] We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) that compared the therapeutic effects of Western medicine (WM) and TCM+WM on patients with glomerulosclerosis.…”