Comparative effect of carotenoid complex from golden neo-life dynamite (GNLD) and carrot ectracted caroteniods on immune parameters in albino wistar rats

Abstract

The immunomodulatory effect of Carotenoid complex from Golden Neo-Life Dynamite (GNLD) and carrot extracted Carotenoid was assessed using 24 albino Wistar rats. The rats were assigned to 4 groups of 6 rats each consisting of group 1(control group treated with distilled water), group 2 (treated with olive oil), group 3 (treated with carrot extract + olive oil) and group 4 (treated with GNLD Carotenoid complex + olive oil). The concentration of carrot extracted Carotenoid and that of the GNLD Carotenoid complex were559mg/kg.The animals were sacrificed after 21 days of treatment and whole blood collected for analysis of immune parameters. The parameters included; percentage lymphocytes, percentage eosinophils, percentage monocytes and platelet count. There were significant increases (P<0.05) in groups 3 and 4 which received carrot extract and Carotenoid complex supplement when compared to group 1 and 2 which received distilled water and olive oil. This supports the claim that Carotenoids have immunomodulatory effects. However, it is clear that there were significant increases (P<0.05) in percentage lymphocytes (40.15± 1.33), monocytes (2.50± 1.31), neutrophils (60.08± 2.06) and platelet count (7.91± 0.53) in group 4 which received carotenoid complex when compared to that of group 3 which received carrot extract; lymphocytes (33.01 ± 3.20), monocytes (1.52 ± 0.52), neutrophils (60.12 ± 1.50) and platelet count (7.81 ± 0.52).These significant increases(P<0.05) show that both carotenoids have immunomodulatory effects; and that the GNLD Carotenoid complex which consists of alpha-carotene, beta-carotene, lutein, Zeaxanthin, beta-cryptoxanthin, lycopene and alpha-tocopherol is more potent in its immunomodulatory effect compared to the carrot extracted carotenoid which contained mostly alphacarotene and beta-carotene alone.

Nutritional and Health Benefits of Carrots and Their Seed Extracts

“…Ekam et al [27] assessed the immunomodullatory effect of carrot-extracted carotenoid using 24 albino rats. The percentage variation in lymphocytes, eosinophils, monocytes and platelet count was evaluated.…”

Edible Vaccines: Promises and Challenges

“…Algal biomass accumulation is very quick, and the whole of the biomass can be used for vaccine production. Green microalgae such as Chlamydomonas reinhardtii as a feasible alternative for vaccine generation [78]. Notwithstanding, a few impediments of plant-derived vaccines, for example, low-expression levels and unsuitable glycosylation of antigen proteins, have been depicted.…”

“…Therefore, as officially portrayed for plantinferred palatable immunizations, the ease and less difficult strategic as far as assembling, stockpiling, conveyance, and organization of the green growth-based innovation make it a perfect framework with regards to asset-restricted settings contrasted with traditional antibody details [82]. There are algae-based vaccines right now in clinical trials; be that as it may, preclinical details against human papillomavirus (HPV), HBV, and foot-and-mouth ailment infection (FMDV) are a work in progress to defeat some specialized issues like low-expression level from the atomic genome and absence of glycosylation following chloroplast expression [78,83]. Research to date show that algae such as Chlamydomonas can produce complex antigens that can stimulate immunogenic responses and are suitable to be developed as vaccines [78].…”

“…There are algae-based vaccines right now in clinical trials; be that as it may, preclinical details against human papillomavirus (HPV), HBV, and foot-and-mouth ailment infection (FMDV) are a work in progress to defeat some specialized issues like low-expression level from the atomic genome and absence of glycosylation following chloroplast expression [78,83]. Research to date show that algae such as Chlamydomonas can produce complex antigens that can stimulate immunogenic responses and are suitable to be developed as vaccines [78].…”

Disease Prevention: An Opportunity to Expand Edible Plant-Based Vaccines?

“…Carrots, along with A. thaliana, were also utilized in experimental edible vaccines for surface HIV antigen expression, and studies performed in rats showed more positive effects in treated animals compared to non-treated animals [81]. The utilization of carrots to treat HIV appears promising not only because carrots are healthy and delicious but also because the consumption of carrot-derived carotenoids increases lymphocytes, monocytes and other immune defenders in rats [127]. Thus, people with weakened immune systems might benefit from consuming this potentially edible anti-HIV vaccine.…”