Comparative effect of silver nanoparticles (AgNPs) derived from actinomycetes and henna on biohydrogen production by Clostridium beijerinckii (KTCC1737)

Abstract

The current study comparatively evaluated the effects of silver nanoparticles (AgNPs) on fermentative bio hydrogen (bio-H 2 ) production by Clostridium beijerinckii (KTCC1737). Green synthesized AgNPs were fabricated using henna (Lawsonia inermis) and DU-C2 actinomycete in batch using different AgNPs concentrations (0, 40, 80, and 120 μg/mL). The overall results revealed that after 72 hours of AgNPs incubation with C beijerinckii, the henna mediated AgNP synthesis increased the hydrogen production rate with a hydrogen yield of 1.71 mol H 2 /mol glucose added . However, the DU-C2-mediated AgNps synthesis inhibited bio-H 2 production during the batch H 2 fermentation. Performed live/dead staining showed AgNPs effect on the bacterial culture in batch fermentation. The results indicated that the henna mediated AgNPs enhanced the bacterial cell population, meanwhile, in case of DU-C2 AgNPs, less bacterial population occurred. Thus, the present study provides a clear overview on the application of metallic NPs as H 2 production catalysts to further improve bio-H 2 production.

Back to nature: henna extracts from nanotech to environmental biotechnology – a review

“…The results implied that the B20 blend, which reduced the amount of 20% pure diesel fuel, could be utilized in place of plain diesel if preferred. Khan et al (2021) employed henna and actinomycete DU-C2 in the production of AgNPs for biohydrogen production. In batch configurations, Clostridium beijerinckii was incubated with henna-mediated AgNPs at concentrations of 0, 40, 80, and 120 g/ml for 72 h, resulting in an increase in hydrogen production with a yield of 1.71 mol H2/mol added glucose.…”

Enhancement of biological hydrogen production from organic wastes with the application of nanomaterials

“…Green silver nanoparticles are examined as the best catalyst for biohydrogen production. Khan et al 86 performed biohydrogen production with the supplementation of silver nanoparticles by C beijerinckii . The green silver nanoparticles were synthesized with henna plant ( Lawsonia inermis ) and actinomycete.…”

Nanomaterials: stimulants for biofuels and renewables, yield and energy optimization

“…The fermentation was confirmed to be changed to ethanol-type from butyrate-type through respective bacterial abundance. Khan et al 154 demonstrated that green synthesized Ag nanoparticles through two ways namely henna with C beijerinckii KTCC and actinomycetes showed varied effects on biohydrogen production. The latter inhibited whereas former increased the hydrogen yield after 72 h incubation period.…”