Comparative Effect of Ulvan and Biosynthesized Silver Nanoparticles on Different Cell Lines Cytotoxicity and Gene Expression

Abstract

Until now, researchers searching for compounds that contribute for treating the most serious disease of this age, cancer. Researchers hope to contribute to this through this research. Activities of both Ulvan extracted from Ulva lactuca and silver nanoparticles (Ag-NPs) were examined against seven different cancer cell types called (Caco2 cells, A549 cells, Mcf7 cells, Hep2 cells, HepG2 cells, PC3 cells, HELA cells). Green synthesized Ag-NPs; via Ulva Lactuca; was characterized by UV-VIS, SEM and TEM. Cell viability assay was conducted to study cytotoxicity effects of both Ulvan and Ag-NPs treatments. RTPCR; using different sense and antisense primers; was carried out to study the expression of GAPDH, Bcl-2, Bax, P21 and P53 (apoptosis related genes) in treated cell lines. SEM and TEM showed that Ag-NPs shape is spherical with average size of 3.89-55 nm. Also, cell viability study showed that both Ulvan and Ag-NPs have cytotoxicity effects on different cancer cells. However, Ag-NPs are more effective than Ulvan. Gene expression data illustrated that both treatments induced down-regulation of Bcl2 and induced up-regulation of P53 genes. This work showed that both Ulvan and Ag-NPs have activities against the investigated cancer cell types.

Safe Ulvan Silver Nanoparticles Composite Films for Active Food Packaging

“…Nanoparticles do their work through their chemical, physical and biological properties, because of their small size and large surface size compared to their large counterparts (Cano et al, 2018). Silver Nanoparticles (Ag-NPs) for their antimicrobial properties have further been used in many fields, in agriculture as nanopesticides (Amin, 2020a) in medicine such as bandages, tooth paste and anticancer (Amin and Abdelreheem, 2020) in food industries such as containers for food storage, air filters, cosmetic and paints (Cano et al, 2018). Ag-NPs enhance the film barrier properties, make the film stronger and increase the hydrophobicity of films, so bio-nanotechnology should be used in food packaging (Nile et al, 2020).…”

“…Oceans and seas are rich in algae which had been used as human food in many countries in the world especially in Asia and have a major role in the industry such as fertilizer, biofuels and pesticides (Yu-Qing et al, 2016) as animal feed (Abd El-Galil and Amin, 2017) in medicine as anti-tumor drugs (Amin et al, 2015) and in the food processing industry products such as fish balls, meatballs, nuggets, broad bean beans and cooked horse beans (Meshhal, 2018). U. lactuca is a green macro alga that is called green lavers or sea lettuce is characterized by many beneficial phytochemicals (Amin, 2019) and is also characterized by polysaccharides that show good anti-microbial and antioxidants activities (Amin, 2020b) Its polysaccharides have a unique chemical structure that can't be found in higher plants or other algae called ulvan and is used in medicine for fighting many diseases (Yu-Qing et al, 2016) such as against cancer (Amin and Abdelreheem, 2020), in the bio-control activity of many microbes (Rivas-Garcia et al, 2018) and is also used as prebiotic in food industry (Shalaby and Amin, 2019) and in food packaging ) Guidara et al, 2020). Biopolymers such as polysaccharide, ulvan, are biocompatible, biodegradable, non-toxic, highly stable and safe for use in the food packaging industry and delivery of nutraceutical products (Ahmed and Ali, 2020).…”