Comparative effectiveness of bioassay methods in identifying the most virulent entomopathogenic fungal strains to control Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)

Sain, S. K.; Monga, D.; Kumar, Rishi; Nagrale, Dipak T.; Kranthi, S.; Kranthi, K. R.

doi:10.1186/s41938-019-0130-z

Cited by 15 publications

(11 citation statements)

References 20 publications

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“…Fungal isolates are effective biological tools against many target pests of controlled and semi-controlled glass-houses without causing remarkable loss to other biocontrol agents and pollinators. Various studies have found entomopathogens as a strong tool of biological control of sucking insect pests (Amnuaykanjanasin et al 2013;Yun et al 2017;El Husseini 2019c;Mohamed 2019;Sain et al 2019).…”

Section: Resultsmentioning

confidence: 99%

Pathogenicity of some entomopathogenic fungal strains to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae)

Javed

Mukhtar

et al. 2019

Egypt J Biol Pest Control

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Pathogenicity of four fungal strains, two of Beauveria bassiana (designated as BB-72 and BB-252), and two of Lecanicillium lecanii (designated as V-4 and CS-625) to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae) was assessed. All treatments showed highly significant effects on the mortality of the aphid when applied as filtrate or conidia. The application methods did not have a significant effect on the mortality rate. The overall mortalities caused by both applications were statistically similar. The maximum overall mortality was caused by BB-72, followed by CS-625 in both application methods. On the other hand, (V-4) caused the minimum mortality rate, followed by (BB-252), which was statistically similar to those caused by V-4. The time also had significant effects on aphid mortality rates. The mortality was the minimum after 3 days reaching its maximum after 10 days. As the number of days increased, there was a corresponding increase in the mortality showing a direct relationship between mortality and time. The maximum mean individual mortality of 95% was caused by CS-625, followed by BB-72 (92%) after 10 days with the conidial application. Similarly, the combined effects of the three most virulent fungal strains showed highly significant differences on the mortality of peach aphid. The combination BB-72 + BB-252 showed the highest percent mortality, followed by BB-72 + BB-252 + CS-625. The combinations BB-72 + CS-625 and BB-252 + CS-625 had statistically similar effects of causing aphid mortality.

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Section: Resultsmentioning

confidence: 99%

Pathogenicity of some entomopathogenic fungal strains to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae)

Javed

Mukhtar

et al. 2019

Egypt J Biol Pest Control

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“…There are relatively few whitefly toxicological bioassay studies where multiple methods were compared in the same project, mainly to assess either adult or immature whitefly response [ 14 , 33 ]. Sain et al [ 34 ] found that there was an advantage of using an intact leaf compared to multiple methods using leaf disk or detached leaf bioassays. Our results were similar in that the one detached leaf bioassay used, the dish method, provided the poorest dose response data for whiteflies.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Toxicological Bioassays for Whiteflies

Sparks

Riley

Simmons

et al. 2020

Insects

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Two Bemisia tabaci populations from Georgia and Florida, USA, were tested for their response to insecticides across different toxicological bioassay methods. Five insecticides in four Insecticide Resistance Action Committee (IRAC) groups (imidacloprid (4A), dinotefuran (4A), flupyradifurone (4D), pyriproxyfen (7C) and cyantraniliprole (28)), were evaluated against a water check. The routes of application to the plant used were either leaf drench or (systemic) root drench. The four different whitefly bioassay methodologies tested were two published IRAC methods, a clip cage method, and a new tube method. A split–split experimental design was used to assess any interactions between application route, bioassay method and insecticide treatment. Application route had no significant effect on efficacy. However, bioassay method affected overall whitefly mortality, with the dish method having reduced mortality compared to other methods, except for the clip cage method. High rates of cyantraniliprole, dinotefuran and flupyradifurone insecticides resulted in the highest incidence of adult whitefly mortality. Significant interactions relative to percent adult mortality were found between the insecticide and bioassay method for both populations assayed. The clip cage method was more sensitive in terms of dose mortality response followed by the cup and tube methods. The dish method was the least responsive to insecticide dose. Other interactions are discussed.

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“…The highest corrected mortality, after 4 days, with the highest concentration was 22.5%. Sain et al (2019) reported that the conidial concentration of C. javanica strains at 10 7 ml −1 caused 77.6-81.1% mortality in second to third instars of B. tabaci nymphs. An LC 50 value of 10 7 spores ml −1 after 4 days of inoculation has been reported against nymphs of the green peach aphid (Myzus persicae) (Kang et al 2018).…”

Section: Resultsmentioning

confidence: 99%

Efficacy of an Omani strain ofCordyceps javanicaand its culture filtrate against whitefly (Bemisia tabaci) under laboratory conditions

et al. 2020

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In this study, an entomopathogenic fungus was isolated from whitefly (Bemisia tabaci) cadaver samples collected from eggplant fields in Barka, Oman. The fungus was identified as Cordyceps javanica based on sequence analysis of ribosomal DNA internal transcribed spacer (ITS) regions. Under in vitro conditions, the fungus was able to grow between 20°C and 30°C, with a maximum growth at 30°C. No growth occurred at 35°C. Studies on the efficacy of different concentrations of conidia of C. javanica against whitefly nymphs revealed that the mortality rate increased with the increase in concentrations of inoculum. About 95.6 ± 2.1% corrected mortality was recorded at a concentration of 10 7 conidia ml −1 after 7 days of treatment. The culture filtrate of C. javanica exhibited toxicity against whitefly nymphs and recorded 61.3 ± 9.5% corrected mortality after 48 h of treatment. There was no significant difference between the mortality caused by conidial suspension and culture filtrate. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of 17 compounds in the culture filtrate of C. javanica. Lauric acid was present in the largest amount (28.7%) and the long chain (C22-25) hydrocarbons contributed 38.3%. This is the first report on the isolation and characterization of C. javanica from B. tabaci from Oman.

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Comparative effectiveness of bioassay methods in identifying the most virulent entomopathogenic fungal strains to control Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)

Cited by 15 publications

References 20 publications

Pathogenicity of some entomopathogenic fungal strains to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae)

Pathogenicity of some entomopathogenic fungal strains to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae)

Comparison of Toxicological Bioassays for Whiteflies

Efficacy of an Omani strain ofCordyceps javanicaand its culture filtrate against whitefly (Bemisia tabaci) under laboratory conditions

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