Comparative efficacy of a multi-epitope DNA vaccine via intranasal, peroral, and intramuscular delivery against lethal Toxoplasma gondii infection in mice

Cong, Hua; Yuan, Quan; Zhao, Qunli; Zhao, Lingxiao; Yin, Huiquan; Zhou, Huaiyu; He, Shenyi; Wang, Zhiyu

doi:10.1186/1756-3305-7-145

Cited by 34 publications

(25 citation statements)

References 37 publications

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“…This novel delivery system has proven successful in inducing protective immunity against many infections such as Toxoplasma gondii [7], Giardia lamblia [8] and Trypanosoma cruzi [9]. In our previous study, attenuated Salmonella typhimurium was used to orally deliver a DNA vaccine of Ts 87, an excretory/secretory antigen from T .…”

Section: Introductionmentioning

confidence: 99%

Oral Vaccination with Attenuated Salmonella typhimurium-Delivered TsPmy DNA Vaccine Elicits Protective Immunity against Trichinella spiralis in BALB/c Mice

Wang

et al. 2016

PLoS Negl Trop Dis

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BackgroundOur previous studies showed that Trichinella spiralis paramyosin (TsPmy) is an immunomodulatory protein that inhibits complement C1q and C8/C9 to evade host complement attack. Vaccination with recombinant TsPmy protein induced protective immunity against T. spiralis larval challenge. Due to the difficulty in producing TsPmy as a soluble recombinant protein, we prepared a DNA vaccine as an alternative approach in order to elicit a robust immunity against Trichinella infection.Methods and FindingsThe full-length TsPmy coding DNA was cloned into the eukaryotic expression plasmid pVAX1, and the recombinant pVAX1/TsPmy was transformed into attenuated Salmonella typhimurium strain SL7207. Oral vaccination of mice with this attenuated Salmonella-delivered TsPmy DNA vaccine elicited a significant mucosal sIgA response in the intestine and a systemic IgG antibody response with IgG2a as the predominant subclass. Cytokine analysis also showed a significant increase in the Th1 (IFN-γ, IL-2) and Th2 (IL-4, 5, 6, 10) responses in lymphocytes from the spleen and MLNs of immunized mice upon stimulation with TsPmy protein. The expression of the homing receptors CCR9/CCR10 on antibody secreting B cells may be related to the translocation of IgA-secreted B cells to local intestinal mucosa. The mice immunized with Salmonella-delivered TsPmy DNA vaccine produced a significant 44.8% reduction in adult worm and a 46.6% reduction in muscle larvae after challenge with T. spiralis larvae.ConclusionOur results demonstrated that oral vaccination with TsPmy DNA delivered by live attenuated S. typhimurium elicited a significant local IgA response and a mixed Th1/Th2 immune response that elicited a significant protection against T. spiralis infection in mice.

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Section: Introductionmentioning

confidence: 99%

Oral Vaccination with Attenuated Salmonella typhimurium-Delivered TsPmy DNA Vaccine Elicits Protective Immunity against Trichinella spiralis in BALB/c Mice

Wang

et al. 2016

PLoS Negl Trop Dis

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“…These properties make it an attractive antigen for diagnostics and vaccine development [12]. Having excellent immune features, recombinant SAG1 has been the most studied antigen of Toxoplasma for development of antibody-based serological tests and vaccine [32][33][34]. A previous study by Letillois and collaborators showed that rSAG-1 may be used as a marker of reactivated toxoplasmosis in HIV infected patients [11].…”

Section: Discussionmentioning

confidence: 99%

SELEX-Based Direct Enzyme-Linked Aptamer Assay(DELAA) for Diagnosis of Toxoplasmosis by Detection of SAG1 Antigen in Sera of Mice and Humans

Shen

Cui

Cudjoe

et al. 2020

Preprint

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Background: Toxoplasma gondii is a single-celled parasite commonly found in mammals. Diagnosis of toxoplasmosis largely depends on measurements of the antibody and/or antigen and Toxoplasma-derived DNAs due to the presence of tissue dwelling quiescent cysts and latent infection of the parasite. As a major surface antigen of T.gondii tachyzoites, SAG1 is a key marker for laboratory diagnosis. However, at present, there are no methods available for SAG1 detection using aptamer-based technology.Methods: Recombinant truncated SAG1（r-tSAG1）of Toxoplasma WH3 strain (type Chinese 1) was prokaryotically expressed and subjected to the synthetic oligonucleotide library for selection of nucleic acid aptamer which targets the r-tSAG1, with systematic evolution of ligands by exponential enrichment (SELEX) strategy. The screened specific aptamer-2 was used in direct enzyme-linked aptamer assay (DELAA) to detect native SAG1 obtained from tachyzoite lysates, mouse sera of acute infection, and human sera that had been verified to be positive for ToxoDNAs by PCR amplification. Results: The soluble r-tSAG1 protein was obtained from E.coli lysates by using 0.01M Tris-Cl in PBS, and was purified and identified by immunoblotting, and then labelled with biotin. The screened aptamers were amplified by PCR, followed by DNA sequencing. The results showed that the aptamer-2, with the highest affinity to nSAG1 among the four aptamer candidates, has a higher specificity and sensitivity when used in detection of nSAG1 in the sera of both animals and humans when compared with the commercial Toxoplasma circulating antigen testing kit.Conclusions: A new direct enzyme-linked aptamer assay (DELAA), with aptamer-2 as the recognition probe, was developed for detection of native SAG1 protein secreted by T.gondii. With increased sensitivity and specificity, stability during storage, easy and cheaper production, the aptamer-based technique is considered as a efficient method for the diagnosis of active and reactivated toxoplasmosis.

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“…Similarly, significant increase in the cellular and humoral immunity was generated when the mice was immunized with a multi-epitope vaccine containing two T cell epitopes and one B cell epitope of SAG1, GRA1, and GRA4. In addition, vaccinated mice obtained long-term survival rates compared with the unvaccinated controls [12].…”

Section: Epitope-based Vaccine Against Toxoplasmosismentioning

confidence: 99%

“…Intensive efforts and significant advances toward acquiring an effective vaccine are under way to control infection and limit the incidence of the disease; however, no vaccine has, thus, far been available for use in humans [11][12][13]. Currently, the live attenuated tachyzoites of the strain S48 (commercially named "Toxovax") is the only approved vaccine for veterinary use.…”

Section: Introductionmentioning

confidence: 99%

Epitope-based vaccine as a universal vaccination strategy against Toxoplasma gondii infection: A mini-review

et al. 2019

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Despite the significant progress in the recent efforts toward developing an effective vaccine against toxoplasmosis, the search for new protective vaccination strategy still remains a challenge and elusive goal because it becomes the appropriate way to prevent the disease. Various experimental approaches in the past few years showed that developing a potential vaccine against the disease can be achievable. The combination of multi-epitopes expressing different stages of the parasite life cycle has become an optimal strategy for acquiring a potent, safe, and effective vaccine. Epitope-based vaccines have gained attention as alternative vaccine candidates due to their ability of inducing protective immune responses. This mini-review highlights the current status and the prospects of Toxoplasma gondii vaccine development along with the application of epitope-based vaccine in the future parasite immunization as a novel under development and evaluation strategy.

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Comparative efficacy of a multi-epitope DNA vaccine via intranasal, peroral, and intramuscular delivery against lethal Toxoplasma gondii infection in mice

Cited by 34 publications

References 37 publications

Oral Vaccination with Attenuated Salmonella typhimurium-Delivered TsPmy DNA Vaccine Elicits Protective Immunity against Trichinella spiralis in BALB/c Mice

Oral Vaccination with Attenuated Salmonella typhimurium-Delivered TsPmy DNA Vaccine Elicits Protective Immunity against Trichinella spiralis in BALB/c Mice

SELEX-Based Direct Enzyme-Linked Aptamer Assay(DELAA) for Diagnosis of Toxoplasmosis by Detection of SAG1 Antigen in Sera of Mice and Humans

Epitope-based vaccine as a universal vaccination strategy against Toxoplasma gondii infection: A mini-review

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