Comparative evaluation of angiogenesis using a novel platelet-rich product: An <i>in vitro</i> study

Gomez, Treesa William; Gopal, Rajesh; Gaffoor, Faisal M A; Kumar, Santhosh T.R; Sabari, Girish C; Prakash, R

doi:10.4103/jcd.jcd_216_18

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…These observations were reproduced in embryonic kidney fibroblasts and in various cell lines such as HEK293, MG-63 osteosarcoma cells, human oral keratinocytes, SIRC, and 3T3 cells [18]. Mesenchymal cells, endothelial cells [23,42,55,63], epithelial cells [22], and macrophages [69] also responded to PRF with increasing proliferation. In contrast, PRF failed to induce proliferation of L929 fibroblasts [53] and human mesenchymal stem cells on collagen scaffolds [17].…”

Section: Selection Of Studiesmentioning

confidence: 60%

Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

et al. 2019

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Objective To systematically assess the effects of platelet-rich fibrin (PRF) on in vitro cellular behavior. Methods A systematic electronic search using MEDLINE database was performed. In vitro studies using PRF were considered and articles published up to June 31, 2018 were screened. Eligible studies were selected based on the use of human PRF. Results In total, 1746 titles were identified with the search terms, from these 37 met the inclusion criteria and were chosen for data extraction. In addition, 16 new studies, mainly published in 2019, were also included in the analysis resulting in 53 studies. No meta-analysis could be performed due to the heterogeneity of study designs. Included studies show that PRF enhances proliferation, migration, adhesion, and osteogenic differentiation on a variety of cell types along with cell signaling activation. Furthermore, PRF reduces inflammation, suppresses osteoclastogenesis, and increases the expression of various growth factors in mesenchymal cells. Summary and conclusions Despite some notable differences of the studies, the overall findings suggest a positive effect of PRF on cell proliferation, migration, adhesion, differentiation, and inflammation pointing towards a therapeutic potential in regenerative dentistry. Clinical relevance PRF serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. Although the cellular mechanisms by which PRF supports the clinical outcomes remain unclear, in vitro research provides possible explanations. This systematic review aims to provide an update of the existing research on how PRF affects basic physiological processes in vitro. The overall findings suggest that PRF induces cell proliferation, migration, adhesion, and differentiation along with possessing anti-inflammatory properties further supporting its therapeutic potential in wound healing and bone regeneration.

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Section: Selection Of Studiesmentioning

confidence: 60%

Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

et al. 2019

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“…A previous dose-response study determined that the intracerebroventricular infusion of vascular endothelial growth factor (VEGF) increases vascular density with minimal associated brain edema at a concentration of 5 μg/ml [13]. It was found that titanium-prepared PRP (TPRP) has better angiogenic potential than its counterpart PRP [14]. These novel growth factor concentrates may be further studied for their effects on wound healing and their administration may be incorporated in CINPT.…”

Section: Positive Pressure Infusion With Growth Factor Concentrate Anmentioning

confidence: 99%

Clinical Applications and Benefits of Using Closed-Incision Negative Pressure Therapy for Incision and Surrounding Soft Tissue Management: A Novel Approach for Comorbid Wounds

Gomez¹,

Gomez²,

Gopal³

2020

Cureus

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The principle of negative pressure technique dates back to the earliest civilizations; during the Roman era, the technique of using dome-shaped cupping glasses was used to create the suction needed to promote healing. This technique was used throughout the 19th century. In 1821, a British physician named Dr. Francis Fox invented the "glass leech" technique. Thereafter in 1952, an innovative approach was introduced to the treatment of serious, complex wounds through the use of sub-atmospheric or negative pressure known as "negative pressure wound therapy" (NPWT). Later, the "vacuum-assisted closure", or VAC therapy system founded by Dr. Louis Argenta in 1990 revolutionized the advanced wound care market, and still remains the most clinically proven alternative for the treatment of complex, hard-to-heal wounds. These therapies utilize a foam dressing that is conformed to the wound bed. When sealed and placed under negative (vacuum) pressure, the system creates a unique wound-healing environment that has been shown to promote the wound-healing process, reduce edema, prepare the wound bed for closure, promote the formation of granulation tissue and remove infectious materials. The negative pressure therapy system addresses patient quality of life through an easy-to-use system designed to assist surgeons in the management and treatment of comorbid wounds, and open abdomen and other wound complications to help achieve primary fascial closure. Comorbidities can be defined as a concurrence of multiple chronic diseases in the same patient. Closed-incision negative pressure therapy (CINPT) has revolutionized the way in which caregivers treat the most serious, complex wounds or comorbid wounds. Wound healing can be achieved by the host's innate and adaptive immune defence mechanisms as in an uninfected simple surgical incision through the skin or by combination of the host's defence mechanisms and therapeutic modalities. It has been confirmed in some clinical researches that growth factors exert amazing effects on wound-healing promotion and skin function restoration without any obvious side effects. In this review, we have hypothesized a novel modality, focusing on the treatment of wound complications secondary to comorbidity by a combination of negative pressure therapy followed by a positive pressure infusion with growth factor concentrates.

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“…Also calcium hydroxide has become one of the most popular methods for pulp capping materials to form dentin bridge (2) . Mineral trioxide aggregate MTA has been an acceptable pulp capping material (3) . Moreover; many bio materials such as Platelets Rich Plasma (PRP), Platelets Rich Fibrin (PRF) have been used for direct pulp capping (4) .…”

Section: Introductionmentioning

confidence: 99%

“…So it has been proposed as possible medicament for pulp capping. Platelet-rich fibrin (PRF) is high platelet concentrate which improves soft and hard-tissue healing (3) . PRF has upper hand over the known PRP include ease of processing/application, minimal cost and no anticoagulant is required.…”

Section: Introductionmentioning

confidence: 99%

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Pulp Tissue Response to Platelets Rich Plasma, Platelets Rich Fibrin and Mineral Trioxide Aggregate as Pulp Capping Materials

et al. 2021

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Purpose: to evaluate the biological response of the dog teeth to Platelets Rich plasma (PRP), Platelets Rich fibrin (PRF) and mineral trioxide aggregate (MTA) as pulp capping materials. Material and Methods: A total of 90 teeth of dogs were included. Class V cavities were prepared. Pulp exposure was performed in the middle of cavity floor. The teeth were divided into three groups of 30 teeth according to the material used; A1: PRP, A2: PRF and A3: MTA. Glass ionomer restoration was applied as restoration for the cavities. The tissue response to the materials was assessed at three different post-operative periods of 7, 30, 60 days. The animal was sacrificed. Histomorphometric analysis was performed. Result: the results showed high inflammatory cells count at 7 days for all treated groups; PRP treated teeth showed the highest at 7 days, while PRF showed the least. At 1 and 2 months postoperatively no significant difference was found between all the treated groups. For dentin bridge scores; there was no significant difference between all the materials at 7 days and 1 month. At 2 months; the MTA treated teeth recorded the greatest dentin bridge score as compared to all treated teeth.As for integrity of odontoblast score; results showed no significant difference between different materials at 7 days and 1 month. The MTA treated teeth showed the highest score as compared to PRP and PRF at 2 months. Conclusion: Among the three different materials, PRP and PRF appeared to be promising pulp capping agents.

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Comparative evaluation of angiogenesis using a novel platelet-rich product: An in vitro study

Cited by 5 publications

References 22 publications

Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies

Clinical Applications and Benefits of Using Closed-Incision Negative Pressure Therapy for Incision and Surrounding Soft Tissue Management: A Novel Approach for Comorbid Wounds

Pulp Tissue Response to Platelets Rich Plasma, Platelets Rich Fibrin and Mineral Trioxide Aggregate as Pulp Capping Materials

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