Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats

Waheeb, Neven; Marouf, Sherif; Nasr, Eman; Abdelmalek, Shaymaa

doi:10.17582/journal.aavs/2022/10.3.466.471

Cited by 3 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Asi mismo Waheeb y col. [33], compararon las técnicas de ELISA vs PCR y encontraron la presencia de H. pylori en las heces de perros y gatos, con una tasa de casos positivos 20,5 % por ELISA y 22,7 % por la prueba de PCR. Este valor superior de +2 % indica la clara sensibilidad que posee la prueba del PCR, al necesitar para su diagnóstico, mucha menos cantidad del microorganismo presente en la muestra.…”

Section: Resultados Y Discusionunclassified

“…En la actualidad, los métodos de diagnóstico más utilizados, al no ser invasivos, siendo prácticos, baratos, cómodos y de fácil aplicación son los inmunoensayos enzimáticos y la PCR, ya que son métodos confiables para diagnosticar una infección activa y confirmar un tratamiento efectivo [27,33].…”

Section: Introductionunclassified

“…La prueba de EIA del antígeno de H. pylori (Kit) es un inmunoensayo enzimático de fase sólida basado en el principio de doble sándwich para la detección semicuantitativa del antígeno de H. pylori en heces humanas, no obstante, utilizada en perros y gatos [33]. La metodología consiste en poner en contacto la placa del micropocillo, la cual está recubierta con un anti-H monoclonal (anticuerpos Pylori).…”

Section: Introductionunclassified

See 2 more Smart Citations

Detección de Helicobacter pylori mediante la técnica micro ELISA en muestras fecales en perros (Canis lupus familiaris)

2022

View full text Add to dashboard Cite

Helicobacteriosis es una enfermedad causada por Helycobacter pylori, que se caracteriza por producir inflamación de la mucosa gástrica, provocando gastritis, úlcera péptica o incluso cáncer gástrico. Como reservorio natural del H. pylori ha sido considerado el hombre, no obstante, diversos estudios han confirmado la presencia de bacterias espirales en perros y gatos. Dada la estrecha relación que existe entre mascotas y seres humanos, algunos investigadores apuestan a la posible transmisión zoonótica entre ellos. Para diagnosticar la presencia de H. pylori actualmente hay pruebas de inmunoensayos enzimáticos, no invasivos, prácticos, cómodos y de fácil aplicación. Debido a esta particularidad se realizó la presente investigación, cuyo objetivo fue el diagnóstico de la presencia de antígenos de H. pylori en heces fecales de perros que asistieron a consulta Veterinaria en la ciudad de Cuenca, Ecuador, mediante la técnica de micro ELISA. El análisis estadístico contempló pruebas estadísticas descriptivas y ji-cuadrado; se estudiaron algunos factores predisponentes que se midieron en la consulta veterinaria, tales como el sexo, la edad, tipo de alimentación, compañía de otro animal y el hábitat. Los resultados obtenidos indican que de los 92 pacientes evaluados solo 11 (12 %) de ellos resultaron positivos a la EIA Test Kit®. Estos hallazgos confirman que el H. pylori está ampliamente distribuido en perros. Entre los factores predisponentes estudiados, a excepción de la compañía entre perros y gatos, los demás factores analizados en esta investigación no afectaron la prevalencia de la enfermedad. El sexo aproximó diferencia al 6 %, destacando que la helicobacteriosis es más frecuente en hembras que en machos. Para concluir se confirma la existencia de H. pylori en perros, convirtiéndole por lo tanto en un posible ente de difusión de este germen en la población.

show abstract

Section: Resultados Y Discusionunclassified

Section: Introductionunclassified

See 1 more Smart Citation

Detección de Helicobacter pylori mediante la técnica micro ELISA en muestras fecales en perros (Canis lupus familiaris)

2022

View full text Add to dashboard Cite

show abstract

“…Second run: Initial denaturation at 94°C for 3 minutes, 20 cycles at 94°C for 30 seconds, annealing at 58°C for 30 seconds, extension at 72°C for 30 seconds, and final extension at 72°C for 5 minutes, followed by electrophoresis on a 1% agarose gel. The gel was stained with ethidium bromide and examined under a UV transilluminator for the presence of the amplified DNA (Image Quant 400, GE Healthcare) (Waheeb et al, 2022).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Comparative Assessment of HpSA-LFIA Using a Hemi-Nested 16SrRNA PCR for Diagnosis of H. pylori in Human Stool and Feces of Animals

Wafy

Abdelmalek

Mostafa

2022

Journal of Current Veterinary Research

View full text Add to dashboard Cite

Helicobacter pylori is a zoonotic gut pathogen that affects humans and animals. Half of the world's population is infected with H. pylori. Helicobacter pylori stool antigen lateral flow immunochromatographic assay is the most common rapid test for disease diagnosis. This study aimed to evaluate the commercial Helicobacter pylori stool antigen lateral flow immunochromatographic assay using a gold standard test, a Hemi-nested 16srRNA PCR. Methodology: 200 stool and fecal samples were collected from dyspeptic humans, dogs, and cats. HpSA-LFIA and 16srRNA Hemi-nested PCR were performed on the collected samples. Statistical analysis was applied to get the sensitivity and specificity of the test under evaluation. The sample size was calculated with a power of 80% (online calculator). The stool and fecal samples were preserved at -20°C until the test performance. Results:The obtained Se and Sp were 91.89% and 59.51%, respectively. Conclusion: HpSA-LFIA in our country is a highly sensitive test with low specificity and low accuracy to be the sole test for diagnosis. The test was intended to be used as a screening test and provided a preliminary result which was not enough for precision and final diagnosis.

show abstract

“…Invasive and non-invasive methods are used in the diagnosis of Helicobacter infection. Among invasive methods with endoscopic biopsy, histological examination, culture, rapid urease test (RUT), and polymerase chain reaction (PCR) are shown; while among noninvasive methods, faecal antigen test, urea breath test (UBT), and serological tests are named [5]. Although gastroscopy, which is within the invasive diagnostic methods, has higher sensitivity and specificity than other methods, it should be noted that many veterinarians do not have the opportunity and ability to perform gastroscopy, in addition to the risks of gastroscopy and associated anaesthesia.…”

Section: Introductionmentioning

confidence: 99%

Treatment and Determination of the Presence of Helicobacter in Shelter dogs by Faecal Antigen Testing and Enzyme–Linked Immunosorbent Assay

Gökalp,

Kırbaş,

Sayar

et al. 2024

RC FCV-LUZ

View full text Add to dashboard Cite

In order to determine the presence of Helicobacter in shelter Dogs in Yozgat Province of Türkiye by fecal antigen test and ELISA and to treat the infection during the initial period. As the material of the study, faecal antigen test, faecal samples for ELISA, blood samples for haematological and biochemical analyses were collected from 82 dogs in Sorgun animal shelter where food and water bowls were kept in compartments. Infected and control groups were formed according to the results of the fecal antigen test. As a result of fecal antigen test, 6 of 82 animals were antigen positive and 76 were antigen negative. According to ELISA analysis; 53 dogs were antibody positive and 29 dogs were antibody negative. No significance was determined between the groups in terms of age and gender in both tests (P>0.05). Dogs in the infected group were treated according to a protocol known as triple therapy, which is used in Helicobacter infections. WBC, NEU and MON counts, RDW and % NEU values of the infected group were higher in the hematological examination, while the % LYM values were significantly lower than the control group. In the biochemical examination, the concentrations TNF–α (21.17 pg·mL-1 vs. 48.21 pg·mL-1), IL–1β (73.41 pg·mL-1 vs. 37.60 pg·mL-1) (P<0.01) and CRP (644.0 mg·dL-1 vs. 234.01 mg·dL-1) (P<0.001) were found to be higher than those of the control group. As a result, it was determined in the study that the presence of Helicobacter was intense in dogs that were in one–to–one contact with each other, including the feeding of dogs in shelter conditions. With the methods used in the study, it is predicted that the presence of Helicobacter can be detected and treated in the early stages, thus preventing transmission of Helicobacter in dogs.

show abstract

Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats

Cited by 3 publications

References 20 publications

Detección de Helicobacter pylori mediante la técnica micro ELISA en muestras fecales en perros (Canis lupus familiaris)

Detección de Helicobacter pylori mediante la técnica micro ELISA en muestras fecales en perros (Canis lupus familiaris)

Comparative Assessment of HpSA-LFIA Using a Hemi-Nested 16SrRNA PCR for Diagnosis of H. pylori in Human Stool and Feces of Animals

Treatment and Determination of the Presence of Helicobacter in Shelter dogs by Faecal Antigen Testing and Enzyme–Linked Immunosorbent Assay

Contact Info

Product

Resources

About