Comparative Evaluation of EMB Agar and Hicrome E. coli Agar for Differentiation of Green Metallic Sheen Producing Non E. coli and Typical E. coli Colonies from Food and Environmental Samples

Antony, Ally C.; Paul, Mini; Silvester, Reshma; Aneesa, P A; Korpole, Suresh; Divya, P S; Paul, Simmy; Fathima, P A; Hatha, A. A. Mohamed

doi:10.22207/jpam.10.4.48

Cited by 23 publications

(13 citation statements)

References 16 publications

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“…Isolation and identification of causative pathogens of bovine mastitis by the use of specific media for growth and culture is still considered to be the gold standard (Radostits et al, 2000). However due to the appearance of false positive results and associated less sensitive and efficient results with the use of EMB agar for the detection of E.coli from various kinds of biological and environmental samples, use of modified media like Hicrome E.coli agar and further confirmation by using molecular methods like PCR has been recommended by various researchers (Antony et al, 2016;Islam et al, 2014). All the clinical bovine mastitis milk samples were used for the isolation and identification of gram negative bacteria using Mueller Hinton agar.…”

Section: Resultsmentioning

confidence: 99%

Incidence of Bovine Clinical Mastitis Caused by Escherichia coli

Manasa¹,

Kumar²,

Rao³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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Section: Resultsmentioning

confidence: 99%

Incidence of Bovine Clinical Mastitis Caused by Escherichia coli

Manasa¹,

Kumar²,

Rao³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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“…Similar lack of green metallic sheen has been reported for culture of milk samples 56 and culture of E. coli with interference of whitish colonies. 57 Overall, these results indicate that our acoustofluidic device is comparable with the standard method in maintaining bacterial cells’ proliferation ability.…”

Section: Resultsmentioning

confidence: 52%

On-chip stool liquefaction via acoustofluidics

Zhao

et al. 2019

Lab Chip

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“…The abundance of these subspecies in effluent from WWTP is in agreement with previous studies on wastewater samples from municipal wastewater plants (Yu and Zhang, 2012; Cydzik-Kwiatkowska and Zielinska, 2016). On EMB agar, the Gram negative colonies showing dark spots and a metallic green sheen were identified as Escherichia subspecies (Antony et al, 2016) while the clear Gram negative colonies on EMB agar were found to be Pseudomonas subspecies (Chiang et al, 2010) upon further analysis with 16S rRNA sequencing. Similarly, the Gram-positive isolates on TSA plates were identified as Streptococcus subspecies, while large pink colonies were identified as Klebsiella spp on MacConkey agar (Bagley and Seidler, 1978), and large yellow colonies were identified to be Staphylococcus spp.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Detection of Bacterial Community and Its Drug-Resistance Profiling From Local Reclaimed Wastewater Plants

Limayem

Wasson

Mehta

et al. 2019

Front. Cell. Infect. Microbiol.

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Treated wastewater from reclaimed facilities (WWTP) has become a reusable source for a variety of applications, such as agricultural irrigation. However, it is also a potential reservoir of clinically-relevant multidrug resistant (MDR) pathogens, including ESKAPE (Enterococcus faecium and Streptococcus surrogates, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species along with the emerging nosocomial Escherichia strains). This study was performed to decipher the bacterial community structure through Illumina high throughput 16S rRNA gene sequencing, and to determine the resistance profile using the Sensititre antimicrobial susceptibility test (AST) conforming to clinical lab standards (NCCLS). Out of 1747 bacterial strains detected from wastewater influent and effluent, Pseudomonas was the most predominant genus related to ESKAPE in influent, with sequence reads corresponding to 21.356%, followed by Streptococcus (6.445%), Acinetobacter (0.968%), Enterococcus (0.063%), Klebsiella (0.038%), Escherichia (0.028%) and Staphylococcus (0.004%). Despite the different treatment methods used, the effluent still revealed the presence of some Pseudomonas strains (0.066%), and a wide range of gram-positive cocci, including Staphylococcus (0.194%), Streptococcus (0.63%) and Enterococcus (0.037%), in addition to gram-negative Acinetobacter (0.736%), Klebsiella (0.1%), and Escherichia sub-species (0.811%). The AST results indicated that the strains Escherichia along with Klebsiella and Acinetobacter, isolated from the effluent, displayed resistance to 11 antibiotics, while Pseudomonas was resistant to 7 antibiotics, and Streptococcus along with Staphylococcus were resistant to 9 antibiotics. Results herein, proved the existence of some nosocomial MDR pathogens, known for ESKAPE, with potential drug resistance transfer to the non-pathogen microbes, requiring targeted remediation.

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Comparative Evaluation of EMB Agar and Hicrome E. coli Agar for Differentiation of Green Metallic Sheen Producing Non E. coli and Typical E. coli Colonies from Food and Environmental Samples

Cited by 23 publications

References 16 publications

Incidence of Bovine Clinical Mastitis Caused by Escherichia coli

Incidence of Bovine Clinical Mastitis Caused by Escherichia coli

On-chip stool liquefaction via acoustofluidics

High-Throughput Detection of Bacterial Community and Its Drug-Resistance Profiling From Local Reclaimed Wastewater Plants

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