Comparative evaluation of growth factors from platelet concentrates: An in vitro study

Chatterjee, Anirban; Debnath, Koel

doi:10.4103/jisp.jisp_678_18

Cited by 24 publications

(11 citation statements)

References 34 publications

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“…The PRFs release a high amount of growth factors including transforming growth factor-β1 and β2 (TGFβ-1 and TGFβ-2), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor 1 and 2 (IGF-1 and IGF-2), fibroblasts growth factor (FGF), and matrix glycoproteins (such as thrombospondin-1) for at least 7 days in vitro. [10][11][12] The PDGF occurs in the form of both homodimers (PDGF-AA and PDGF-BB) and heterodimers (PDGF-AB), and influences the synthesis of DNA strains, inducing angiogenesis, chemotaxis, and mitogenesis of fibroblasts, osteoblasts and monocytes. It cooperates with IGFs, which, in turn, leads to the differentiation of fibroblasts into osteoblasts, further increasing the amount of bone collagen in osteoblasts.…”

Section: Introductionmentioning

confidence: 99%

PRF versus xenograft in sinus augmentation in case of HA-coating implant placement: A 36-months retrospective study

Dominiak¹,

Karuga-Kuźniewska²,

Popecki³

et al. 2021

Adv Clin Exp Med

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Background. Sinus lift with a simultaneous implant placement in the residual maxilla is a common technique used worldwide. Nevertheless, choosing an ideal grafting material remains an object of dispute. The use of an autologous blood-derived graft, known as platelet-rich fibrin (PRF), has not yet been recognized to be as good as xenografts and alloplastic materials. However, initial results have been promising.Objectives. To conduct a clinical and radiological comparison of implantation with a simultaneous sinus lift using xenograft or PRF clots. Materials and methods.Thirty sinus lifts with simultaneous implantation were conducted using a lateral window approach and the tent pole technique, with xenograft (group 1 (G 1 )) or PRF (group 2 (G 2 )) as a filling material. To be included in the study, patients must have had an alveolar ridge height of 4-5 mm, no signs of inflammatory processes, good oral hygiene, and no other grafting procedures performed in region of implant insertion. In each case, the measurements taken were probing pocket depth (PPD), height of keratinized tissue (HKT), clinical attachment level (CAL), recession depth/width (RD/RW), and, on panoramic X-rays, marginal bone loss (MBL), grafted sinus high (GSH), and bone gain (BG). Pre-and post-operative treatment was applied to reduce the chance of infection.Results. During the study, 30 implants (hydroxyapatite-coated implants manufactured by SGS -10 mm in length and 4.2 mm in diameter) were placed. The survival rate of implants in both groups was 100% with no implant mobility, pain, paresthesia, or inflammatory processes in the direct vicinity of the implants observed, except in 1 patient. After 36 months of follow-up, the radiological assessments for G 1 were: GSH 4.5 mm, MBL 0.46 mm and BG 4.53 mm; and for G 2 : 3.4 mm, 0.6 mm and 3.4 mm, respectively. Results of the clinical measurements were for G 1 : HKT after 36 months (HKT 36 ) 2.46 mm, CAL 0.47 mm and PPD 2 mm; and for G 2 : HKT 36 3. 13 mm, CAL 0.6 mm and PPD 2.07 mm. Conclusions.After 3 years of follow-up, the results of sinus lifting solely using PRF with simultaneous implantation were promising, especially in terms of soft tissue management. Therefore, PRF can be regarded as an alternative to previously used materials.

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Section: Introductionmentioning

confidence: 99%

PRF versus xenograft in sinus augmentation in case of HA-coating implant placement: A 36-months retrospective study

Dominiak¹,

Karuga-Kuźniewska²,

Popecki³

et al. 2021

Adv Clin Exp Med

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“…To the best of our knowledge only one paper [52] compared PRFM and PRF products, in terms of growth factor release. In this study, PRFM and PRF were obtained by "home-made" protocols and appeared to have a different kinetic release.…”

Section: Platelet-rich Fibrinmentioning

confidence: 99%

Platelet Concentrates in Musculoskeletal Medicine

Mariani

Pulsatelli

2020

IJMS

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Platelet concentrates (PCs), mostly represented by platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are autologous biological blood-derived products that may combine plasma/platelet-derived bioactive components, together with fibrin-forming protein able to create a natural three-dimensional scaffold. These types of products are safely used in clinical applications due to the autologous-derived source and the minimally invasive application procedure. In this narrative review, we focus on three main topics concerning the use of platelet concentrate for treating musculoskeletal conditions: (a) the different procedures to prepare PCs, (b) the composition of PCs that is related to the type of methodological procedure adopted and (c) the clinical application in musculoskeletal medicine, efficacy and main limits of the different studies.

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“…Суттєва роль у репаративному остеогенезі належить тромбоцитам (Zhang et al, 2012;Saikrishna & Yelamali, 2015;Burianov et al, 2017;Ho-Shui-Ling et al, 2018;Chatterjee & Debnath, 2019;Kornsuthisopon et al, 2020) завдяки дії їх паракринних біомолекулфакторів росту та цитокінів (Bansal et al, 2017;Shevchenko et al, 2019), які виконують певну роль у процесах відновлення пошкоджених структур: запаленні, синтезі колагену та ініціації ангіогенезу (Pascale et al, 2015;Neiva et al, 2016). Власне завдяки цьому перспективними у наданні остеокондуктивним матеріалам остеоіндуктивних і остеогенних властивостей вважаються різні технологічні варіанти аутологічних тромбоцитарних матеріалів.…”

Section: вступunclassified

“…TGF-β1 є одним з найважливіших факторів росту, який виділяється тромбоцитами під час загоєння кісток і сприяє проліферації фібробластів, стовбурових клітин кісткового мозку, преостеобластів і активності остеокластів. Крім того, судинно-ендотеліальний фактор росту (VEGF) покращує ангіогенез, що є фундаментальним елементом за регенерації кістки (Peric et al, 2015;Pascale et al, 2015;Chatterjee & Debnath, 2019;Kornsuthisopon et al, 2020).…”

Section: результати та їх обговоренняunclassified

“…Попередні дослідження на модельних тваринах засвідчили позитивний вплив фібрину, збагаченого тромбоцитами, на прискорення регенерації в комбінації з неорганічним компонентом кісток ВРХ, для загоєння внутрішньокісткових дефектів щелепи (Chatterjee & Debnath, 2019;Chou et al, 2020).…”

Section: результати та їх обговоренняunclassified

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Histomorphological characteristics of bone replacement in rabbits with hydroxyapatite ceramics and Platelet-Rich Fibrin

et al. 2021

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The results of a histomorphological study of bone regenerates in rabbits after osteosubstitution with platelet-rich fibrin and its combination with hydroxyapatite granules with β-tricalcium phosphate in spongy and compact bone tissue at different periods of reparative osteogenesis are presented. Three groups of rabbits were formed. In the control group, the defects were left to heal under the blood clot. Bone lesions in the first experimental group were filled with platelet-rich fibrin, in the second – with a combination of platelet-rich fibrin and hydroxyapatite with β-tricalcium phosphate. All animals were kept in the same conditions of feeding and housing, had unlimited access to water. During the study, the rabbits were monitored clinically. The animals were taken out of the experiment on the 14th, 21st and 42nd days, samples of bone tissue were taken, they were fixed, decalcified, dehydrated in alcohols of increasing concentration and embedded in paraffin. In the presented study, it is most likely that the newly formed bone tissue is formed precisely due to osteoinduction in the experimental groups. When using granules of hydroxyapatite and β-tricalcium phosphate with platelet-rich fibrin, bone regenerate is between the composite granules and is not associated with contact with the maternal bone. Its cells appear in different places of the defect. In the spongy bone tissue on the 21st day, regeneration proceeded more fully and faster in the second experimental group, as evidenced by the significant density of cells of the osteoblastic row, the thickness of the bone trabeculae and their volume, filling the site of the defect. On the 42nd day, in the second experimental group, when using a combination of autobiomaterial and hydroxyapatite granules with β-tricalcium phosphate, the regenerate contained a significantly larger number of osteogenic cells in the thickness of the trabeculae, which indicated a more intensive course of reparative osteogenesis in comparison with the first experimental and control groups. In the compact bone on day 21, regeneration was more complete and faster in the second experimental group. On the 42nd day, according to the degree of maturity of the bone regenerate, calcium-phosphate ceramics in combination with platelet-rich fibrin optimizes reparative osteogenesis most clearly. According to the degree of intensity of the osteoregeneration process, the groups can be placed in the following sequence: control ˂PRF˂PRF+HA/β-TCP–700. According to histomorphological characteristics, the combination of hydroxyapatite with β-tricalcium phosphate and platelet-rich fibrin gives greater osteoinduction to the composite material, which is confirmed by the high cell density, namely of osteoblasts and osteocytes. The use of PRF in combination with other materials may become promising for the correction of reparative osteogenesis in conditions of limited or reduced regenerative potential of bone tissue.

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Comparative evaluation of growth factors from platelet concentrates: An in vitro study

Cited by 24 publications

References 34 publications

PRF versus xenograft in sinus augmentation in case of HA-coating implant placement: A 36-months retrospective study

PRF versus xenograft in sinus augmentation in case of HA-coating implant placement: A 36-months retrospective study

Platelet Concentrates in Musculoskeletal Medicine

Histomorphological characteristics of bone replacement in rabbits with hydroxyapatite ceramics and Platelet-Rich Fibrin

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