Comparative evaluation of immunological and structural similarities of snake venom C-type lectin proteins

Castro, Helena C.; Lemos, Manuela; Bon, Cassian; Zingali, Russolina B.

doi:10.1016/s0041-0101(02)00358-6

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…GPIb-BP has a high similarity to botrocetin, and was reported to induce thrombocytopenia in mice and guinea pigs [ 42 ]. Interestingly, despite the high amino acid similarity between botrocetin, bothrojaracin, and jararaca IX/X binding protein found in BjV, polyclonal antibodies anti-native bothrojaracin, a CTLP that inhibits thrombin, shows low cross-reactivity with native botrocetin and jararaca IX/X binding protein, indicating a higher degree of selectivity of antibodies [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom

et al. 2021

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Patients bitten by snakes consistently manifest a bleeding tendency, in which thrombocytopenia, consumption coagulopathy, mucous bleeding, and, more rarely, thrombotic microangiopathy, are observed. Von Willebrand factor (VWF) is required for primary hemostasis, and some venom proteins, such as botrocetin (a C-type lectin-like protein) and snake venom metalloproteinases (SVMP), disturb the normal interaction between platelets and VWF, possibly contributing to snakebite-induced bleedings. To understand the relationship among plasma VWF, platelets, botrocetin and SVMP from Bothrops jararaca snake venom (BjV) in the development of thrombocytopenia, we used (a) Wistar rats injected s.c. with BjV preincubated with anti-botrocetin antibodies (ABA) and/or Na2-EDTA (a SVMP inhibitor), and (b) VWF knockout mice (Vwf-/-) injected with BjV. Under all conditions, BjV induced a rapid and intense thrombocytopenia. In rats, BjV alone reduced the levels of VWF:Ag, VWF:CB, high molecular weight multimers of VWF, ADAMTS13 activity, and factor VIII. Moreover, VWF:Ag levels in rats that received BjV preincubated with Na2-EDTA and/or ABA tended to recover faster. In mice, BjV caused thrombocytopenia in both Vwf-/- and C57BL/6 (background control) strains, and VWF:Ag levels tended to decrease in C57BL/6, demonstrating that thrombocytopenia was independent of the presence of plasma VWF. These findings showed that botrocetin present in BjV failed to affect the extent or the time course of thrombocytopenia induced by envenomation, but it contributed to decrease the levels and function of plasma VWF. Thus, VWF alterations during B. jararaca envenomation are an ancillary event, and not the main mechanism leading to decreased platelet counts.

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Section: Introductionmentioning

confidence: 99%

Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom

et al. 2021

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“…One such domain has been purified from Bothrops jararacussu, Bothrops jararaca and Crotalus atrox venom and characterized as a C-type galactoside-binding lectin (9,10). They have erythrocyte-agglutinating activity and other properties, such as adhesion to plasmatic proteins that affect blood homeostasis by inhibiting or activating specific platelet membrane receptors and blood coagulation factors (11,12).…”

Section: Introductionmentioning

confidence: 99%

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

Barbosa¹,

Martins²,

Toyama³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis pv. passiflorae treated with lectin disclosed a high vesiculation and membrane rupture. BmLec induced a strong and significant increase in insulin secretion at 2.8 and 16.7 mM glucose concentrations, and this effect was seen in the presence of EGTA in both experiments. BmLec (10 µg/mL) increased the perfusion pressure, renal vascular resistance and urinary flow. The glomerular filtration rate and percentages of sodium, potassium and chloride tubular transport were reduced at 60 minutes of perfusion. Renal alterations caused by BmLec were completely inhibited by indomethacin in all evaluated parameters. In conclusion, the C-type lectin isolated from Bothrops moojeni affected platelet aggregation, insulin secretion, antibacterial activity and isolated kidney function

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“…studied so far. Interestingly, common epitopes are also shared with high-molecularweight lectin-like molecules such as convulxin [15] .…”

Section: Introductionmentioning

confidence: 99%

Bothrojaracin, a <i>Bothrops jararaca</i> Snake Venom-Derived (Pro)Thrombin Inhibitor, as an Anti-Thrombotic Molecule

Zingali

Ferreira

Assafim

et al. 2005

Pathophysiol Haemos Thromb

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Bothrojaracin (BJC) is a selective and potent thrombin inhibitor (K_D = 0.6 nM) which also binds to prothrombin on proexosite I (K_D = 175 nM). Incubation of BJC with human or rat plasma produced a band that co-migrates with purified prothrombin-BJC complex. We further analyzed the in vivo anti-thrombotic effect of BJC on a venous thrombosis model in rats that combines stasis and hypercoagulability. The administration of 1 mg/kg (i.v.) doses of BJC decreased thrombus weight by ∼95%. Evaluation of the in vivo effect of BJC in mice using a pulmonary thromboembolism model induced by thrombin showed that BJC protects 100% of mice from death. Altogether, our data show that BJC is a potent anti-thrombotic agent that could further help the development of new prothrombin-directed drugs.

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Comparative evaluation of immunological and structural similarities of snake venom C-type lectin proteins

Cited by 8 publications

References 21 publications

Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom

Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom

Purification and biological effects of a C-type lectin isolated from Bothrops moojeni

Bothrojaracin, a <i>Bothrops jararaca</i> Snake Venom-Derived (Pro)Thrombin Inhibitor, as an Anti-Thrombotic Molecule

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