Comparative Evaluation of in vitro Anti-Inflammatory Activity of Different Extracts of Selected Medicinal Plants from Saurashtra Region, Gujarat

Modi, Chirag M.; Bhatt, Punit R.; Pandya, Kajal B.; Patel, Harshad B.; Patel, Urvesh D.

doi:10.20546/ijcmas.2019.805.195

Cited by 12 publications

(6 citation statements)

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“…The main advantages of medicinal plants are, they are economical, readily available and has no side effects. Many medicinal plants from Saurashtra region are reported to have antidiabetic, antibacterial, anti-inflammatory and antioxidant effects (Kaneria et al, 2009;Modi et al, 2019;.…”

Section: Introductionmentioning

confidence: 99%

“…Many synthetic drugs like indomethacin, aspirin, ibuprofen, diclofenac and aceclofenac have potent anti-inflammatory action, but due to significant side effects like kidney failure, damage to the liver and upper GIT bleeding, many drugs are either banned or less used for long term treatment (Patidar et al, 2014). Medicinal plants are a rich source of a variety of phytochemicals like saponin, triterpenoid, flavonoids, alkaloids, which possess anti-inflammatory action via different mechanisms like inhibition COX enzymes, albumin denaturation and protease inhibition (Fawole et al, 2010;Modi et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Antidiabetic, antioxidant and anti-inflammatory activity of medicinal plants collected from nearby area of Junagadh, Gujarat

Bhatt¹,

Pandya²,

Patel³

et al. 2019

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The present study was planned to carry out phytochemical analysis and to evaluate in vitro antidiabetic, antioxidant and anti-inflammatory activities of different extracts of six medicinal plants collected from the nearby area of Junagadh, Gujarat, India, viz., Luffa echinata Roxb. (fruit), Operculina turpethum (L.) Silva Manso (leaf), Sphaeranthus indicus L. (fruit), Cressa cretica L. (leaf), Corchorus depressus L. (root) and Cassia absus (seed). An in vitro antidiabetic activity was evaluated by alpha-amylase and alpha-glucosidase inhibition methods, antioxidant activity by DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay and anti-inflammatory activity by albumin denaturation assay. Hydro-alcoholic and methanolic extracts of L.echinata, water extract of C.cretica and methanolic and hydro-alcoholic extracts of C. depressus showed significant inhibition either of alpha-amylase or alpha-glucosidase or both in vitro at various concentrations. Hydro-alcoholic and methanolic extracts of L. echinata fruit and hydroalcoholic and methanolic extracts of S. indicus fruit showed significant in vitro antioxidant effect. Water extract of O. turpethum leaf showed significant anti-inflammatory activity by preventing albumin denaturation in vitro. Phytochemical analysis revealed the presence of glycosides, flavonoids, alkaloids, tannins and steroids in different extracts of medicinal plants which might be responsible for significant in vitro antidiabetic, antioxidant and antiinflammatory activities. Identification and isolation of active substances from such plants and in vivo efficacy evaluation will be helpful for further validation of pharmacological activities of these medicinal plants.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antidiabetic, antioxidant and anti-inflammatory activity of medicinal plants collected from nearby area of Junagadh, Gujarat

Bhatt¹,

Pandya²,

Patel³

et al. 2019

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“…This allows them to react as reducing agents, hydrogen donors and singlet oxygen quenchers [44]. Modi et al (2019) reported that the mechanism of protein denaturation changes the tertiary and secondary structures of protein due to alteration in electrostatic forces due to hydrophobia, disulphide bonds, and hydrogen [45], and some chemical and physical agents, like alcohol, acetone, acids, alkalies, heavy metal salts, dyes [46], heat, light and pressure, can also alter the protein denaturation. Mizushima and Kobayashi, in 1968, reported that the inflammatory drugs salicylic acid, phenylbutazone, etc., had a dose-dependent impact on thermally induced protein denaturation.…”

Section: Discussionmentioning

confidence: 99%

Anti-Arthritic and Anti-Inflammatory Potential of Spondias mangifera Extract Fractions: An In Silico, In Vitro and In Vivo Approach

Khalid

Alqarni

Shoaib

et al. 2021

Plants

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The fruits of Spondias mangifera (S. mangifera) have traditionally been used for the management of rheumatism in the northeast region of India. The present study explores the probable anti-arthritis and anti-inflammatory potential of S. mangifera fruit extract’s ethanolic fraction (EtoH-F). To support this study, we first approached the parameters in silico by means of the active constituents of the plant (beta amyrin, beta sitosterol, oleonolic acid and co-crystallised ligands, i.e., SPD-304) via molecular docking on COX-1, COX-2 and TNF-α. Thereafter, the absorption, distribution, metabolism, excretion and toxicity properties were also determined, and finally experimental activity was performed in vitro and in vivo. The in vitro activities of the plant extract fractions were evaluated by means of parameters like 1,1-Diphenyl-2- picrylhydrazyl (DPPH), free radical-reducing potential, albumin denaturation, and protease inhibitory activity. The in vivo activity was evaluated using parameters like COX, TNF-α and IL-6 inhibition assay and arthritis score in Freund Adjuvant (CFA) models at a dose of 400 mg/kg b.w. per day of different fractions (hexane, chloroform, alcoholic). The molecular docking assay was performed on COX-1, COX-2 and TNF-α. The results of in vitro studies showed concentration-dependent reduction in albumin denaturation, protease inhibitors and scavenging activity at 500 µg/mL. Administration of the S. mangifera alcoholic fraction at the abovementioned dose resulted in a significant reduction (p < 0.01) in arthritis score, paw diameters, TNF-α, IL-6 as compared to diseased animals. The docking results showed that residues show a critical binding affinity with TNF-α and act as the TNF-α antagonist. The alcoholic fraction of S. mangifera extract possesses beneficial effects on rheumatoid arthritis as well as anti-inflammatory potential, and can further can be used as a possible agent for novel target-based therapies for the management of arthritis.

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“…The antitrypsin activity was noticed more for seed ethanol extract (3.14%) than for Leaf ethanol extract (1.26%). Solanum xanthocarpum aerial part water extract has been demonstrated to have 67% protease inhibition at a concentration of 0.5 mg/mL concentration 22 . For proteinase inhibitory action, aqueous extract of Solanum aethiopicum has an IC 50 value of 19.85 μg/ml compared to the standard aspirin with IC 50 value of 9.35 μg/ml 23 .…”

Section: Fig 1: In-vitro Anti-inflammatory Activity Of Standard Aspir...mentioning

confidence: 99%

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2024

IJPSR

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In view of rising concern of problem associated with synthetic drugs against inflammation, current research has been focused towards safer, effective and natural compounds. Hence, we aimed at assessing the antiinflammatory effect of four plants from Solanum species (S. seaforthianum, S. diphyllum, S. sisymbriifollium and S. anguivi) through albumin denaturation assay, trypsin inhibition and RBC membrane stabilization assays. The data showed that the methanol extracts of these plants had concentration dependent protection. S. anguivi extract showed maximum inhibition of albumin denaturation (26.15 ± 0.08%), whereas S. sisymbriifollium extract displayed maximum (16.51 ± 0.002%) trypsin inhibition. With respect to heat induced RBC lysis, S. anguivi extract showed highest inhibition (26.16 ± 0.08%), whereas S. seaforthianum extract displayed maximum inhibition (36.49 ± 0.002%) of RBC lysis induced by hypotonicity. From the study, it can be inferred that anti-inflammatory activity in the tested Solanum species extract varied considerably and its activity can be attributed to its richness in phytochemical compounds.

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Comparative Evaluation of in vitro Anti-Inflammatory Activity of Different Extracts of Selected Medicinal Plants from Saurashtra Region, Gujarat

Cited by 12 publications

References 6 publications

Antidiabetic, antioxidant and anti-inflammatory activity of medicinal plants collected from nearby area of Junagadh, Gujarat

Antidiabetic, antioxidant and anti-inflammatory activity of medicinal plants collected from nearby area of Junagadh, Gujarat

Anti-Arthritic and Anti-Inflammatory Potential of Spondias mangifera Extract Fractions: An In Silico, In Vitro and In Vivo Approach

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