Comparative Evaluation of Lateral Flow Assay and PCR for Detection of Canine Parvovirus

Abstract

P arvoviral gastroenteritis is a highly contagious viral disease which causes severe acute haemorrhagic enteritis and myocarditis in puppies over the age of 3-4 months. The disease is spread from dog to dog mainly through exposure to contaminated faeces and is caused by CPV-2. It is also spread through contact with fomites (contaminated objects). It is also spread through contact with fomites. Further, large pool of unvaccinated apparently healthy stray dogs may act as carriers without showing any symptoms and become source of infection to other susceptible dogs. The stools will be watery, yellow in colour or tinged with frank blood. Faeces can vary from soft in mild cases to grossly hemorrhagic in severe cases. Death ensues due to dehydration, leading to the peripheral circulatory failure. Parvovirus myocarditis is a condition of disease is caused by Canine parvovirus-2 (CPV-2). The virus can remain on a dog's hair coat and serve as a means of transmission long after dogs have recovered young dogs and primarily a lit

Comparison of pre-labelled primers and nucleotides as DNA labelling method for lateral flow detection of Legionella pneumophila amplicons

“…For example, PCR as NAAT (Nucleic Acid Amplification Technology) is mainly used in laboratories due to its complexity and requirements for trained staff, but also due to its extremely high sensitivity and reliability. In contrast, simple lateral flow assays (LFA) can be carried out by non-professionals in the privacy of one's own home, are less expensive and provide a quick result, as the use of pregnancy tests and rapid corona tests still impressively demonstrates [11][12][13][14] .In recent years, and especially since isothermal amplification methods have eliminated the need for wellequipped laboratories when it comes to nucleic acid amplification, these two methods are being increasingly combined [15][16][17][18][19][20][21] . The result is a nucleic acid-based lateral flow detection which unites the essential advantages of both methods.…”

“…For example, PCR as NAAT (Nucleic Acid Amplification Technology) is mainly used in laboratories due to its complexity and requirements for trained staff, but also due to its extremely high sensitivity and reliability. In contrast, simple lateral flow assays (LFA) can be carried out by non-professionals in the privacy of one's own home, are less expensive and provide a quick result, as the use of pregnancy tests and rapid corona tests still impressively demonstrates [11][12][13][14] .…”