Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Şevik, Murat; Öztürk, Fahri Ferudun

doi:10.3906/vet-1303-26

Cited by 10 publications

(7 citation statements)

References 19 publications

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“…The internationally accepted methods for the detection of antibody response after vaccination are virus neutralization test (VNT), LPB-ELISA and solid phase competition ELISA [ 10 ]. In the present study, LPB-ELISA was standardized and used for antigenic characterization of CSFV isolates and compared the result with neutralization peroxidase linked assay (NPLA) and both the tests showed positive correlation [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of specific humoral immune response in pigs vaccinated with cell culture adapted classical swine fever vaccine

et al. 2016

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Aim:To determine an efficient vaccination schedule on the basis of the humoral immune response of cell culture adapted live classical swine fever virus (CSFV) vaccinated pigs and maternally derived antibody (MDA) in piglets of vaccinated sows.Materials and Methods:A cell culture adapted live CSFV vaccine was subjected to different vaccination schedule in the present study. Serum samples were collected before vaccination (day 0) and 7, 14, 28, 42, 56, 180, 194, 208, 270, 284 and 298 days after vaccination and were analyzed by liquid phase blocking enzyme-linked immunosorbent assay. Moreover, MDA titre was detected in the serum of piglets at 21 and 42 days of age after farrowing of the vaccinated sows.Results:On 28 days after vaccination, serum samples of 83.33% vaccinated pigs showed the desirable level of antibody titer (log10 1.50 at 1:32 dilution), whereas 100% animals showed log10 1.50 at 1:32 dilution after 42 days of vaccination. Animals received a booster dose at 28 and 180 days post vaccination showed stable high-level antibody titre till the end of the study period. Further, piglets born from pigs vaccinated 1 month after conception showed the desirable level of MDA up to 42 days of age.Conclusion:CSF causes major losses in pig industry. Lapinised vaccines against CSFV are used routinely in endemic countries. In the present study, a cell culture adapted live attenuated vaccine has been evaluated. Based on the level of humoral immune response of vaccinated pigs and MDA titer in piglets born from immunized sows, it may be concluded that the more effective vaccination schedule for prevention of CSF is primary vaccination at 2 months of age followed by booster vaccination at 28 and 180 days post primary vaccination and at 1 month of gestation.

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Section: Discussionmentioning

confidence: 99%

Evaluation of specific humoral immune response in pigs vaccinated with cell culture adapted classical swine fever vaccine

et al. 2016

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“…There are several specific ELISAs for detecting the non-structural proteins (NSP) of FMDV such as Mab trapping (MAT) ELISA and blocking ELISA for detecting of antibodies against 3ABC-NSP (Sorensen et al, 1998). Currently, competitive-ELISA is used for detecting NSP-FMD antibodies and this serological test is more sensitive and rapid than the virus neutralization test (Sevik and Ozturk, 2013). In addition, RT-PCR technique has become established as a confirmatory test.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Risk Factors of Foot and Mouth Disease Virus in Nineveh Province, Iraq

Salim¹,

Talb²,

Yousif³

et al. 2019

Adv. Anim. Vet. Sci.

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| The objectives of the present research are to evaluate the prevalence of foot and mouth disease (FMD) in cattle employing competitive enzyme-linked immunosorbent assay (c-ELISA) and conventional reverse transcriptase polymerase chain reaction (RT-PCR technique), to identify FMDV genotypes and to investigate some epidemiological risk factors related to the disease prevalence. A total of 460 saliva swabs and blood samples were collected from cattle of different ages and sexes in Nineveh province, Iraq. Epidemiological data were collected through interviews with the farmer s' employer. Viral RNA was extracted from saliva, and RT-PCR was conducted to identify the virus genotypes based on VP1 and VP3 genes amplification. The overall occurrence of FMD in Nineveh province based on c-ELISA test and RT-PCR was 46.95% and 40.43%, respectively. According to Kappa value (0.81), it has a mean that is of higher compatibility between c-ELISA test and RT-PCR for diagnosis of FMDV in cattle. The risk factors that are significantly associated with higher prevalence of FMD including: Feedlot calves >6 months -2 years old, females, pregnant animals, imported animals, indoor feeding, large herd size, beef purpose, non-vaccinated, in north-eastern parts of the province and in spring and winter months. Four genotypes of FMDV (A, O, Asia1 and SAT) were detected in Nineveh province at (12.39%, 6.95%, 17.39% and 3.69%) respectively. Genotype C was not detected in this research. In conclusion, our results reported the first time detection of four FMDV genotypes in Nineveh province and there are several risk factors linked to the disease occurrence, which require strict control measures. Citation | Salim SAS, Talb OQ, Yousif AS, Daher HS (2020). Prevalence and risk factors of foot and mouth disease virus in nineveh province, iraq. Adv. Anim. Vet. Sci. 8(1): 1-10.

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“…All data for nodes P2 and P3 were collected from the Russian Federation Service for Veterinary and Phytosanitary Surveillance. The minimum and maximum values for diagnostic sensitivity were taken from a study of virus detection using ELISA (P4) [ 40 , 41 ]. Referring to the international standards (AS/NZS ISO 31,000:2009 Risk management - principles and guidelines), combined with the risk identification, analysis and evaluation methods and risk management process proposed in Chinese national standards (GB/T27921-2011 Risk management - Risk assessment techniques), a semi-quantitative model of FMD positive cattle export caused by the failure of Russian control measures was established.…”

Section: Methodsmentioning

confidence: 99%

Risk assessment and integrated surveillance of foot-and-mouth disease outbreaks in Russia based on Monte Carlo simulation

et al. 2021

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Background Foot-and-mouth disease (FMD) is a highly contagious disease of livestock worldwide. Russia is a big agricultural country with a wide geographical area where FMD outbreaks have become an obstacle for the development of the animal and animal products trade. In this study, we aimed to assess the export risk of FMD from Russia. Results After simulation by Monte Carlo, the results showed that the probability of cattle infected with FMD in the surveillance zone (Surrounding the areas where no epidemic disease has occurred within the prescribed time limit, the construction of buffer areas is called surveillance zone.) of Russia was 1.29 × 10− 6. The probability that at least one FMD positive case was exported from Russia per year in the surveillance zone was 6 %. The predicted number of positive cattle of the 39,530 - 50,576 exported from Russia per year was 0.06. A key node in the impact model was the probability of occurrence of FMD outbreaks in the Russian surveillance zone. By semi-quantitative model calculation, the risk probability of FMD defense system defects was 1.84 × 10− 5, indicating that there was a potential risk in the prevention and control measures of FMD in Russia. The spatial time scan model found that the most likely FMD cluster (P < 0.01) was in the Eastern and Siberian Central regions. Conclusions There was a risk of FMDV among cattle exported from Russia, and the infection rate of cattle in the monitored area was the key factor. Understanding the export risk of FMD in Russia and relevant epidemic prevention measures will help policymakers to develop targeted surveillance plans.

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Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Cited by 10 publications

References 19 publications

Evaluation of specific humoral immune response in pigs vaccinated with cell culture adapted classical swine fever vaccine

Evaluation of specific humoral immune response in pigs vaccinated with cell culture adapted classical swine fever vaccine

Prevalence and Risk Factors of Foot and Mouth Disease Virus in Nineveh Province, Iraq

Risk assessment and integrated surveillance of foot-and-mouth disease outbreaks in Russia based on Monte Carlo simulation

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