Comparative Evaluation of Protein Hydrolysates in the Process of Constructing Based on Them Nutrient Medium for Brucella Cultivating

Ковтун, Ю. С.; Курилова, А. А.; Катунина, Л. С.; Василенко, Е. И.

doi:10.21055/0370-1069-2016-4-93-97

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…в ряде работ показана возможность успешного культивирования бруцелл на таких источниках азотистого питания, как ферментативные гидролизаты крови, желатина, сои, соевого концентрата, глютена кукурузного, рыбной кормовой муки, плацентарноэмбрионально-маточный гидролизат, дрожжевой автолизат, патока и др. [16][17][18][19][20][21]. зарубежные среды аналогичного назначения в качестве белковой основы включают ферментативные гидролизаты казеина, пептические перевары животной ткани, папаиновые перевары соевой муки, бактериологические пептоны и триптозу.…”

Section: питательные среды для диагностики бруцеллезаunclassified

Nutrient Media for Diagnosis of Brucellosis

Ковтун

Курилова

Катунина

et al. 2020

Problemy Osobo Opasnykh Infektsii

Self Cite

View full text Add to dashboard Cite

The overview contains information from Russian and foreign literature about composition and usage of nutrient media for brucellosis diagnosis and immunobiological preparations production. Nutrient media for cultivation, isolation, enrichment, identification and differentiation of Brucella and their L-form as well as transport media and synthetic media are considered. Advantages and disadvantages are described for some of the products. The overview also contains information about nutritional bases, growth factors and selective components included in these media. There is a particular focus on selective media, their history and improvement. The article gives the requirements for the inhibitory properties of these products and the disadvantages of using certain antimicrobial agents. The authors list nutrient media for various purposes, certified for use by the Russian regulatory documents, recommendations of World Health Organization and World Organization for Animal Health. Data about principles for assessing the quality of culture media and test strains used to control biological parameters of culture media are covered. The authors also present materials about the trends in nutrient media development for Brucella cultivation and isolation, methods of their application. In conclusion, they identify the need to conduct research in the sphere of the development of new nutrient media for Brucella isolation from the material contaminated by foreign microorganisms.

show abstract

Section: питательные среды для диагностики бруцеллезаunclassified

Nutrient Media for Diagnosis of Brucellosis

Ковтун

Курилова

Катунина

et al. 2020

Problemy Osobo Opasnykh Infektsii

Self Cite

View full text Add to dashboard Cite

show abstract

“…Наличие в ПС хлорид-ионов или сульфат-ионов, накапливаемых при использовании кислотных гидролизатов, избирательно для ряда микроорганизмов. Избыточное количество этих ионов бывает нежелательно и может ингибировать биотехнологические процессы [1, [13][14][15][16].…”

unclassified

Development of the technology for preparation of enzymatic hydrolysate of waste chick embryos

Овсянников¹,

Дурсенев²

2021

Biological Products. Prevention, Diagnosis, Treatment

View full text Add to dashboard Cite

The development of technologies for preparation of protein nutritional bases for microbiological nutrient media, from production waste of mainly readily available or non-food products, is a promising area in biotechnology. Researchers of Vyatka State Agrotechnological University assume that non-food secondary raw materials, such as waste chick embryos (WCEs) used in the production of anti-influenza products, could be used for these purposes, after removal of the virus-containing allantoic fluid. The aim of the study was to develop a technology for preparation of WCE enzymatic hydrolysate (WCEEH), and to evaluate growth properties of the hydrolysate-based solid nutrient medium, using Escherichia coli M-17 and Pseudomonas alcaligenes IP-1 test strains. Materials and methods: the authors offer methodological approaches to obtaining WCEEH and substantiate hydrolysis parameters. The obtained WCEEH was characterised in terms of physico-chemical properties: pH, amine nitrogen, total nitrogen, sodium chloride, degree of protein cleavage. The growth properties of the hydrolysate-based nutrient medium were studied using E. coli M-17 and Ps. alcaligenes IP-1 test strains. Results: the experiments demonstrated the feasibility of performing enzymatic hydrolysis of WCEs, and assessed physico-chemical properties of the prepared WCEEH batches. The study demonstrated the possibility of using the prepared hydrolysate as a component of solid nutrient media for growing the selected test strains. Conclusions: the study substantiated the optimal technological parameters for WCE enzymatic hydrolysis: pH (7.6 ± 0.3), duration (48 ± 2 h), temperature (49 ± 1) °C. The loading of hydrolysis components was optimised: mass fraction of the substrate—500 g/L, mass fraction of the hydrolysing agent—100 g/L. The physico-chemical properties of WCEEH make it suitable for preparation of microbiological media; the hydrolysate-based solid nutrient medium consistently ensures the growth of E. coli M-17 and Ps. alcaligenes IP-1 test strains with standard properties. The growth properties of the experimental medium are comparable to those of the meat-peptone broth-based nutrient medium.

show abstract

Assessment of the Use of Gentian Violet and Malachite Green as Selective Agents in the Isolation of Brucella

Ковтун

Курилова

Катунина

et al. 2021

Problemy Osobo Opasnykh Infektsii

View full text Add to dashboard Cite

The aim of the work is to evaluate ability of gentian violet and malachite green to inhibit 2 Brucella test strains and 8 strains of unwanted organisms and the inﬂuence of autoclaving on their activity. The study also aims to evaluate variability of inhibition in diﬀerent batches of colorants and necessity in adjustment of their concentration.Materials and methods. The study included 3 imported lots of gentian violet and malachite green. Inhibitors were put into nutrient medium containing pancreatic hydrolysates of gelatin and fsh meal, yeast extract, sodium chloride, glucose, sodium pyrosulfate, and agar. The same medium without colorants was used as a control medium. Eﬀect of the colorants was studied with the help of test strains of Brucella abortus 19 ВА, B. melitensis Rev I, Enterococcus faecium VKM B-602, Escherichia coli ATCC 25922, Proteus vulgaris HX 19 222, Pseudomonas aeruginosa 22/99, Serracia plymuthica 1, Shigella ﬂexneri 1a 8516, Staphylococcus aureus ATCC 25923, Yersinia enterocolitica C-187.Results and discussion. We have determined biological parameters of nutrient media containing gentian violet (5,0; 2,5 1,25 mg/L) and malachite green (2.0; 1.0; 0.5 mg/L) when cultivating brucella. Gentian violet is able to inhibit Brucella greatly at concentration of 5 mg/L. S. aureus ATCC 25923 did not show any growth on the media containing malachite green or gential violet (5.0 and 2.5 mg/L). Malachite green inhibited the growth of enterococcus more actively than gentian violet. However, it inhibited swarming growth of Proteus less successfully than gentian violet. Other unwanted organisms were not sensitive to such concentrations of colorants at all. We have demonstrated the necessity to combine colorants with other selective agents, as well as advisability to put gentian violet into sterile media, while malachite green – into the media before its autoclaving. Adjustments to the concentration of gentian violet should be made when producing nutrient medium, as variations in inhibiting activity in diﬀerent batches of this colorant were revealed.

show abstract

Comparative Evaluation of Protein Hydrolysates in the Process of Constructing Based on Them Nutrient Medium for Brucella Cultivating

Cited by 3 publications

References 0 publications

Nutrient Media for Diagnosis of Brucellosis

Nutrient Media for Diagnosis of Brucellosis

Development of the technology for preparation of enzymatic hydrolysate of waste chick embryos

Assessment of the Use of Gentian Violet and Malachite Green as Selective Agents in the Isolation of Brucella

Contact Info

Product

Resources

About

Comparative Evaluation of Protein Hydrolysates in the Process of Constructing Based on Them Nutrient Medium for Brucella Cultivating

Cited by 3 publications

References 0 publications

Nutrient Media for Diagnosis of Brucellosis

Nutrient Media for Diagnosis of Brucellosis

Development of the technology for preparation of enzymatic hydrolysate of waste chick embryos

Assessment of the Use of Gentian Violet and Malachite Green as Selective Agents in the Isolation of Brucella

Contact Info

Product

Resources

About

Comparative Evaluation of Protein Hydrolysates in the Process of Constructing Based on Them Nutrient Medium for Brucella Cultivating