Comparative evaluation of the antibacterial effects of four dentine bonding systems: An in vitro study

Abstract

Aim:The aim of this in vitro study was to evaluate and the compare antibacterial efficacy of four dentin bonding system against Streptococcus mutans, Streptococcus salivarius and Lactobacillus acidophilus over a period of three months using agar disk diffusion test.Methodology:All the three standard bacterial strains were inoculated into BHI broth and incubated at 37°C for 48 hours. A 100 μl of broth suspension containing aliquots of S. mutans, S. salivarius and L. acidophilus were spread onto M-H agar medium using sterile cotton swabs. The experimental groups were as follows: GROUP A: Test conducted for evaluation of antimicrobial efficacy against S. mutans MTCC 497, GROUP B: Test conducted for evaluation of antimicrobial efficacy against S. salivarius MTCC 1938, and GROUP C: Test conducted for evaluation of antimicrobial efficacy against L. acidophilus MTCC 447. For sample preparation, 20 μl bonding agent was dropped with micropipettes on paper disks, and blown dry for 10 seconds. Then it was light-cured at 2 mm for 20 sec using a QTH visible light curing unit. For first reading, the sample disks were placed over the freshly inoculated agar plates and then incubated at 37°C for 48 hours. The rest of the paper disks were stored in dark, submerged in distilled water at 37°C. They were placed on freshly inoculated spread plates after specific time intervals- 1 day, 1 week, 2 weeks, 1 month and 3 months after ageing in PBS. After incubation, the diameters of zones of inhibition around the plates were measured. The experiment was performed twice in triplicate. The data was then statistically analysed using Two way ANOVA test and post hoc.Results:Results showed that Xeno III had the maximum antibacterial efficacy over a period of three months, followed by XP bond. This antibacterial activity was maximum against Streptococcus mutans, followed by Lactobacillus acidophilus and least against Streptococcus salivarius. Adper Easy One and G bond had minimal effect against the test bacteria during the test period.Conclusion:The antibacterial effect decreased over a period of three months for all the dentin bonding systems.

Antimicrobial properties of conventional restorative filling materials and advances in antimicrobial properties of composite resins and glass ionomer cements—A literature review

“…The most commonly used method is the agar diffusion test, due to its simplicity and cost-effectiveness [12]. In fact, the use of this method is very common, when it comes to testing of antimicrobial properties of restorative materials [1,11,12,22,23,25,[28][29][30]33,34,44,65,71,74,[76][77][78]84,87,103,104,111,118,122,144,146,152,170]. Measurement of the area of inhibition has been suggested in order to reduce any misinterpretation of results, due to this test's qualitative nature [25].…”

Comparative evaluation of antibacterial activity of total-etch and self-etch adhesive systems: An ex vitro study

“…[15192627] In this study, 0.2% chlorhexidine showed a very highly significant antibacterial activity against A. viscosus compared to AP and the mean inhibition zone was less than AP in relation to S. mutans and L. acidophilus .…”

An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

“…Another study [ 72 ] proved cinnamon essential oil to possess the highest antibacterial activity against S. mutans among other nine oils (eucalyptol, lime, clove, mint, vinegar, cedar and citrus grass). In addition, Arora and Kaur [ 73 ] observed the antimicrobial activity of clove EO against C. albicans . It was confirmed by the present study, in which clove EOs exhibited significantly higher activity against C. albicans and L. acidophilus than CHX, whereas no significant difference between in activity of clove EO and CHX against S. mutans was found.…”