“…Therefore, in this study, single-rooted teeth with approximatively the same root canal volume were selected. E. faecalis was chosen as a representative endodontic pathogen because it often survives chemomechanical root canal treatment, it is associated with both primary and refractory endodontic infection (22), and is often used in similar studies (18,23). The time taken for E. faecalis colonization or the biofilm formation in the root canals varies between studies (23).…”