Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (<i>Xenopus laevis</i> and <i>Pleurodeles waltl</i>) using the comet assay and the micronucleus test

Mouchet, Florence; Gauthier, Laury; Baudrimont, Magalie; Gonzalez, Patrice; Mailhes, Corinne; Ferrier, Vincent; Devaux, Alain

doi:10.1002/tox.20267

Cited by 60 publications

(34 citation statements)

References 74 publications

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“…This may be different between species and cellular processes (i.e. metabolization/detoxication) (Gauthier et al 2007). As with many pesticides, the potential genotoxic effect of diuron appears to be mediated by the production of oxyradicals during biotransformation processes.…”

Section: Sensitivity Of Palaemon Serratus Spermatozoa Toward Genotoximentioning

confidence: 99%

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Erraud

Bonnard

Duflot

et al. 2017

Environ Sci Pollut Res

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The aim of this study was to adapt the Comet assay in spermatozoa of the marine prawn Palaemon serratus to use it as a marker of sperm quality. Indeed, due to the characteristics of their spermatozoa, the measurement of DNA integrity is one of the few markers which can be transferred to crustaceans to assess the quality of their semen. In the first step, the methods of collecting and maintaining spermatozoa were optimized. Cell survival was estimated during kinetics of preservation (i.e. 1, 2, 4 and 8 h) in various suspension media to define artificial seawater (ASW) as optimal. Several methods in the releasing of spermatozoa from the spermatophore of prawns were estimated with regard to their incidence both on the efficiency of extraction and the survival of cells. Pipetting up and down turned out to be the most successful and the least invasive technique. Secondly, the transfer of Comet assay was optimized by studying various times in both cell lysis (i.e. 1, 6, 18 h) and DNA denaturation (i.e. 15, 30 and 45 min), after in vitro exposure of spermatozoa to an H2O2 gradient as model genotoxicant. Results revealed that a minimum of 1 h in cell lysis and 15 min of DNA denaturation were sufficient to obtain valuable results, linked with a low compaction of DNA in spermatozoa of Palaemon sp. Finally, the sensitivity of P. serratus spermatozoa was assessed after in vitro exposures to model genotoxicants displaying various modes of interaction with DNA (i.e. UV-C, 13.3-79.5 J m; HO, 5-10 μM and MMS, 0.5-5 mM) and some environmental contaminants known or suspected to be genotoxic (i.e. cadmium and diuron, 0.015-1.5 μg L; carbamazepine, 0.1-10 μg L) for invertebrates. The low variability of the baseline level of DNA strand breaks recorded in controls highlighted the robustness of the method. P. serratus spermatozoa displayed significant DNA damage from the lowest doses tested for all model genotoxicants, but conversely, no genotoxic effect of tested environmental contaminants was observed. These results, which are discussed according to the protocol tested in the present study and the comparison with literature data, could suggest a difference in the response or sensitivity of spermatozoa to environmental genotoxicity between invertebrate species, and therefore the interest of Palaemonidae prawns in ecogenotoxicology. In conclusion, the present study underlines the potential of the Comet assay as a marker to assess the contamination impact on the sperm quality in Palaemonidae prawns in view to a potential application for in situ biomonitoring surveys.

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Section: Sensitivity Of Palaemon Serratus Spermatozoa Toward Genotoximentioning

confidence: 99%

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Erraud

Bonnard

Duflot

et al. 2017

Environ Sci Pollut Res

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“…The SCGE test has become extensively valuable as a biomarker in amphibians to monitor contaminated areas (in situ assay) (Burlibasa and Gavrila, 2011;Maselli et al, 2010), as well as for the screening of xenobiotics after direct or indirect exposure (in vivo assay) (Mouchet et al, 2007;Nikoloff et al, 2014b;Pérez-Iglesias et al, 2014;Ruiz de Arcaute et al, 2014). We observed that, regardless of the length of treatment, IMZT acute exposure within the 0.39-1.17 mg/L concentration range increased the frequency of primary DNA lesions estimated by alkaline SCGE, a result opposite that of the MN test.…”

Section: Figmentioning

confidence: 99%

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Iglesias

Soloneski

Nikoloff

et al. 2015

Ecotoxicology and Environmental Safety

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“…have been well documented in a wide range of different species: teratogenesis in Xenopus laevis (Mouchet et al 2006(Mouchet et al , 2007 and carcinogenesis in Syrian hamsters are useful examples of its toxicity (Waalkes and Rehm 1998). In addition, cadmium is irreversibly accumulated into cells, as it has been demonstrated in rat organs and marine mollusks (Roesijadi et al 1996;Haouem et al 2007).…”

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confidence: 96%

Environmentally relevant cadmium concentrations affect development and induce apoptosis of Paracentrotus lividus larvae cultured in vitro

et al. 2008

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Sea urchin embryos and larvae represent suitable model systems on where to investigate the effects of heavy metals on development and cell viability. Here, we tested the toxic effects of low (10(-12 )M), medium (10(-9 )M), and high (10(-6 )M) cadmium chloride concentrations, mimicking unpolluted, moderately and highly polluted seawaters, respectively, on Paracentrotus lividus sea urchins offspring. Larvae were continuously treated from fertilization and inspected at time intervals comprised between 10 and 30 days of development. Delays and/or morphological abnormalities were firstly evident in larvae treated for 15 days with high cadmium (10(-6 )M) and for 25 days with medium cadmium (10(-9 )M). Major defects consisted in the reduction and lack of arms and skeleton elongation. No obvious differences with respect to controls were observed in embryos/larvae exposed to low cadmium (10(-12) M), even after 30 days of exposure. Using in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL) assay on larvae whole mounts, we detected apoptosis after 10 days of treatment with 10(-6) and 10(-9) M CdCl(2,) when no morphological abnormalities were recognizable yet. Supernumerary apoptotic cells were found in arm buds, ciliary bands, and apex. In conclusion, echinoderm embryos and larvae represent candidates of choice for the study of stress and defense mechanisms activated by cadmium exposure.

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Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test

Cited by 60 publications

References 74 publications

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Toxic and genotoxic effects of the imazethapyr-based herbicide formulation Pivot H® on montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae)

Environmentally relevant cadmium concentrations affect development and induce apoptosis of Paracentrotus lividus larvae cultured in vitro

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