Comparative evaluation of two automated systems for nucleic acid extraction of BK virus: NucliSens easyMAG versus BioRobot MDx

High-Throughput Direct Fecal PCR Assay for Detection of Mycobacterium avium subsp. paratuberculosis in Sheep and Cattle

“…The use of a commercial DNA extraction procedure (Qiagen Bio-Sprint 96 one-for-all vet kit) gave high sensitivity as well as highthroughput capacity in a diagnostic laboratory setting. Magnetic bead DNA purification methods generally lead to lower carryover of contaminants or potential PCR inhibitors from a sample, as the method involves the physical removal of the DNA from the remainder of the sample (31)(32)(33)(34).…”

Extraction of viral nucleic acids: Comparison of five automated nucleic acid extraction platforms

“…The insufficient removal of PCR inhibitors was recently reported to be an issue when detection rates of BK virus in urine specimens after extraction on automated extraction platforms were compared. 23 For this study, it should be mentioned that the assay that had been used for pretesting may present as a minor and unavoidable weakness in the study design. When results obtained for the detection of CMV DNA after extraction on automated extraction platforms were compared, less than two-thirds of the samples analyzed gave positive results with all platforms studied.…”

COVID-19 Molecular Testing in Korea: Practical Essentials and Answers From Experts Based on Experiences of Emergency Use Authorization Assays

“…Respiratory virus multiplex PCR should then be performed, and the positive rate of extraction and Ct values of the target viral gene and internal control (IC) for the proposed reagent should be compared with those for the QIAamp Viral RNA Mini Kit extraction reagent. Although there are no definitive criteria for assessment, a reagent is generally considered comparable in performance to that of the QIAamp Viral RNA Mini Kit extraction reagent, if the Ct value difference is ≤1.66 of 0.5 log 10 copies/mL equivalents [ 9 , 10 ].…”