Comparative Experimental Study of Wound Healing in Mice: Pelnac versus Integra

Abstract

Strategies for skin regeneration have been developed to provide effective treatment for cutaneous wounds and disease. Dermal substitutes have been used to cover the lesion to facilitate cell colonization, thereby promoting dermal regeneration. However, very little is known about Pelnac matrix especially at histological level. Therefore, the present work carried out an experimental in vivo comparative analysis between Pelnac and Integra, the most used dermal templates, in a mouse model of full-thickness skin wounds. Histological sections performed at the 3rd, 6th and 9th days after surgery were analyzed with regard to inflammatory response and vascularization. Both templates were completely incorporated in all animals at the end of the analyzed period. Pelnac-treated animals displayed reduced granulation tissue during the first 6 days of treatment compared to the animals treated with Integra at the same time period. The number of inflammatory cells (neutrophils) was similar in both groups during the period, significantly reducing at the end of inflammatory phase (9th day of treatment) consistent with the progression of healing process. In addition, the density of blood vessels was also statistically similar in both matrices. Therefore, the two dermal templates displayed comparable biological behavior in tissue repair. It is noteworthy that this is the first experimental study comparing Pelnac and Integra dermal templates with focus on full-thickness skin wounds.

Utility of a New Artificial Dermis as a Successful Tool in Face and Scalp Reconstruction for Skin Cancer: Analysis of the Efficacy, Safety, and Aesthetic Outcomes

“…e dermal matrix of collagen is almost identical to endogenous collagen which results in poor antigenic response and a lower rate of rejection. In fact, it is gradually replaced by host (endogenous) [15][16][17][18][19][20]. At approximately three weeks, when well-vascularized neodermis is usually formed, the silicone layer may be peeled off and replaced with an autologous thin split-thickness skin graft.…”

Artificial dermal substitutes for tissue regeneration: comparison of the clinical outcomes and histological findings of two templates

“…Indeed, during the process of wound healing, collagen type III acts as a scaffold for fibroblast attachment, and high secretion of collagen type III is promoted by the DS graft. 29 In the Integra-treated tissue, we observed collagen type III fibers specifically localized in the tissue engraftment area with the DS, while in the Pelnac-treated tissue, the collagen type III fibers were homogeneously distributed throughout the dermis. All samples were negative for collagen type I, indicating that prominent mature collagen fibers (type I) were still not detectable 15 days after treatment ( Figure 6(i), (j)).…”

“…Additionally, several biologic and synthetic dermal and epidermal regenerative modalities have recently been investigated to treat fullthickness skin defects and investigate the fundamental characteristics of the biomaterial that closely resembles the natural features of tissue. 3,4,15,16,24,29,30,32 In the present study, we defined tissueengineered skin bio-constructs as DSs that were acellular with xenogeneic synthetic scaffolding and biodegradable material. We observed differences in the composition, structure, and storage adequacy of the two DSs.…”

In vitro Evaluations of Biodegradable Polyacrylamide Grafted Moringa Bark Gum Graft Copolymer (MOG-g- PAAM) as Biomedical and Controlled Drug Delivery Device Synthesized by Microwave Accelerated free Radical Synthesis

“…Foreign body giant cells observed in minor quantity in case of test shown in Figure 11, an inflammatory response is most common at the interface of both tissue and scaffold which is previously reported in several literature. 35,36 These results ensure the non-toxic nature of the graft copolymer GF4 as well as its efficiency to be utilized as a template material for tissue growth respectively.…”