Comparative expression analysis of potential pathogenicity-associated genes of high- and low-virulent Sporisorium scitamineum isolates during interaction with sugarcane

Nalayeni, Kumaravel; Ashwin, N. M. R.; Barnabas, Leonard; Vinodhini, Thiyagarajan; Agisha, V. N.; Sundar, A. Ramesh; Malathi, P.; Viswanathan, R.

doi:10.1007/s13205-021-02893-7

Cited by 7 publications

(3 citation statements)

References 46 publications

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“…Teliospores of a high-virulent S. scitamineum isolate, Ss97009, and a low-virulent isolate, SsV89101, were collected from the smut susceptible sugarcane cultivars, Co 97009 and CoV 89101, respectively, from the experimental farm of ICAR-SBI, Coimbatore, India, and used in this study. Previously, the virulence of these isolates was evaluated by relative virulence testing on a smut susceptible variety and confirmed by differential host experiments ( Barnabas et al., 2018 ; Nalayeni et al., 2021 ). In the present study, single bud sets of 7-month-old Co 97009, a smut susceptible variety was used for plant inoculation experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The disease severity of sugarcane smut varies greatly, ranging from 10-80%, depending on the climatic conditions, the prevalence of pathogen races, and the resistance of the sugarcane varieties under cultivation in a given area ( Monteiro-Vitorello et al., 2018 ; Nalayeni et al., 2021 ). Until now, the most effective management strategy for sugarcane smut is the use of resistant cultivars ( Sundar et al., 2015 ; Sundar et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis of sugarcane reveals differential switching of major defense signaling pathways in response to Sporisorium scitamineum isolates with varying virulent attributes

et al. 2022

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Sugarcane smut caused by the basidiomycetous fungus Sporisorium scitamineum is one of the most devastating diseases that affect sugarcane production, globally. At present, the most practical and effective management strategy for the disease is the cultivation of resistant cultivars. In this connection, a detailed understanding of the host’s defense mechanism in response to smut isolates with varying degrees of virulence at the molecular level would facilitate the development of reliable and durable smut-resistant sugarcane varieties. Hence, in this study, a comparative whole transcriptome analysis was performed employing Illumina RNA-seq in the smut susceptible cultivar Co 97009 inoculated with two distinct S. scitamineum isolates, Ss97009 (high-virulent) and SsV89101 (low-virulent) during the early phases of infection (2 dpi and 5 dpi) and at the phase of sporogenesis (whip emergence) (60 dpi). Though the differential gene expression profiling identified significant transcriptional changes during the early phase of infection in response to both the isolates, the number of differentially expressed genes (DEGs) were more abundant at 60 dpi during interaction with the high virulent isolate Ss97009, as compared to the low virulent isolate SsV89101. Functional analysis of these DEGs revealed that a majority of them were associated with hormone signaling and the synthesis of defense-related metabolites, suggesting a complex network of defense mechanisms is being operated in response to specific isolates of the smut pathogen. For instance, up-regulation of hormone-related genes, transcription factors, and flavonoid biosynthesis pathway genes was observed in response to both the isolates in the early phase of interaction. In comparison to early phases of infection, only a few pathogenesis-related proteins were up-regulated at 60 dpi in response to Ss97009, which might have rendered the host susceptible to infection. Strikingly, few other carbohydrate metabolism-associated genes like invertases were up-regulated in Ss97009 inoculated plants during the whip emergence stage, representing a shift from sucrose storage to smut symptoms. Altogether, this study established the major switching of defense signaling pathways in response to S. scitamineum isolates with different virulence attributes and provided novel insights into the molecular mechanisms of sugarcane-smut interaction.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of sugarcane reveals differential switching of major defense signaling pathways in response to Sporisorium scitamineum isolates with varying virulent attributes

et al. 2022

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“…The low virulent strains possess some dormancy in the initial growth stages. This difference can often be seen during the comparison of the developmental stages of the pathogen isolates in-vitro (Nalayeni et al, 2021;.…”

Section: Introductionmentioning

confidence: 99%

The Morphological And Molecular Variability Of Sporisorium Scitamineum Isolates In Eswatini

Nkhabindze

Earnshaw

Wanyika

et al. 2022

Preprint

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Background: Sugarcane smut, caused by Sporisorium scitamineum, is a disease of economic importance in the sugarcane industry, occasioning losses of up to 50%. Current management practices have been shown to be ineffective in controlling the fungal disease and hence the understanding of the local pathogen and development of appropriate control measures is required. This study investigates the morphology, development patterns and molecular variability of Sporisorium scitamineum isolates in Eswatini to understand its pathogenicity for effective control.Results: Fungal isolates were collected along the Sugarbelt in Lowveld of Eswatini. The isolates were verified by polymerase chain reaction (PCR) using the bE 4 and bE 8 specific primers with an amplification of a 420bp fragment; this was further verified by the sequencing results. The teliospores from the isolates were uniform for the brown colour, spiny texture and circular shape. The teliospore sizes were significantly (P=0.05) different among the isolates. The isolate from Big-Bend had a mean diameter of 5.55µm, while Simunye, Nsoko and Mhlume had average diameters of 4.69µm, 4.98µm and 4.87µm, respectively. The documentation of the developmental stages revealed that the samples were of variable virulence with significantly (P=0.05) different rates of promycelium development. Genetic distance matrix analysis and the cluster analysis showed a high homology (99-100%) among the local isolates.Conclusion: There is very low variability in the strains that are found in the selected sugarcane growing areas in Eswatini. Local isolates may possess varying virulence levels, but may not require variable management strategies.

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Synergistic Integration of Sugarcane Proteomics with Genomics: Proteogenomics to Decipher the Mechanism of Disease Resistance in Sugarcane

Ashwin¹,

Agisha²,

Vinodhini³

et al. 2022

Agro-Industrial Perspectives on Sugarcane Production Under Environmental Stress

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Comparative expression analysis of potential pathogenicity-associated genes of high- and low-virulent Sporisorium scitamineum isolates during interaction with sugarcane

Cited by 7 publications

References 46 publications

Transcriptome analysis of sugarcane reveals differential switching of major defense signaling pathways in response to Sporisorium scitamineum isolates with varying virulent attributes

Transcriptome analysis of sugarcane reveals differential switching of major defense signaling pathways in response to Sporisorium scitamineum isolates with varying virulent attributes

The Morphological And Molecular Variability Of Sporisorium Scitamineum Isolates In Eswatini

Synergistic Integration of Sugarcane Proteomics with Genomics: Proteogenomics to Decipher the Mechanism of Disease Resistance in Sugarcane

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