Comparative Extraction and Quantifi cation of Myricetin from Leaves of Madhuca longifolia Using RP-HPLC

Abstract

Background: This study’s purpose was to develop a sensitive, repeatable reverse-phase high-performance liquid chromatography (RP-HPLC) method for extracting the main fl avonoid Myricetin from the leaves of Madhuca longifolia and quantifying it. Materials and Methods: Diff erent leaves extracts were prepared using various solvent systems, including ethanol, ethanol: water (50:50), and water, through various extraction methodologies, including hot and cold maceration and soxhlet-assisted extraction, in order to optimize the best solvent system and ideal extraction methodology (SAE). Myricetin was separated by chromatography using a C18 column. Results: According to the fi ndings, the hydroalcoholic extract had the greatest content of myricetin at 2.524 ng. This study reports the presence of fl avonoid content in M. longifolia leaves extract that can be extracted using, water:ethanol and ethanol. The best solvent system was water:ethanol, and the best extraction technique was cold maceration. Conclusion: M. longifolia extracts were produced by hot and cold maceration, SAE techniques, and their fl avonoid concentrations were measured using HPLC-ESI-MS/MS. Water: Cold maceration and ethanol were shown to be promising solvent systems. While trying to extract myricetin from M. longifolia leaves, the extraction approach was more eff ective than other traditional extraction methods.