Comparative fluorescence study of cell membranes and reconstituted liposomes

Methyl-β-Cyclodextrins and Liposomes as Water-Soluble Carriers for Cholesterol Incorporation into Membranes and Its Evaluation by a Microenzymatic Fluorescence Assay and Membrane Fluidity-Sensitive Dyes

“…As hydrophilic carrier complexes, cholesterol-rich dispersions (10,11), cholesterol-loaded liposomes (12), or cholesterol-coated Sephadex beads (13) have been applied. Among these methods, cholesterol-loaded liposomes is the most popular because of easy processing, but different incorporation results are reported (11,12,14,15). Recently, cyclodextrins have been reported to mediate membranal cholesterol content very efficiently (16,17).…”