Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans

Lee, Hyo Seol; Lee, Jongeun; Kim, Seong‐Oh; Song, Je Seon; Lee, Jae Ho; Lee, Syng Ill; Jung, Han‐Sung; Choi, Byung Jai

doi:10.1371/journal.pone.0084201

Cited by 33 publications

(33 citation statements)

References 30 publications

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“…Thrombospondin-4 is a transmembrane CAM, which was the only upregulated CAMs apart from periostin and thus may have an important role. It has been detected in the PDL (Lee et al, 2013) but its function was mostly studied in the context of cancer, where it promotes cell proliferation and migration (Chen et al, 2019, p. 4).…”

Section: Discussionmentioning

confidence: 99%

Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function

et al. 2020

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The pre-occlusal eruption brings the molars into functional occlusion and initiates tensional strains during mastication. We hypothesized that upon establishment of occlusal contact, the periodontal ligament (PDL) undergoes cell and extracellular matrix maturation to adapt to this mechanical function. The PDL of 12 Wistar male rats were laser microdissected to observe the proteomic changes between stages of preocclusal eruption, initial occlusal contact and 1-week after occlusion. The proteome was screened by mass spectrometry and confirmed by immunofluorescence. The PDL underwent maturation upon establishment of occlusion. Downregulation of alphafetoprotein stem cell marker and protein synthesis markers indicate cell differentiation. Upregulated proteins were components of the extracellular matrix (ECM) and were characterized with the matrisome project database. In particular, periostin, a major protein of the PDL, was induced following occlusal contact and localized around collagen α-1 (III) bundles. This co-localization coincided with organization of collagen fibers in direction of the occlusal forces. Establishment of occlusion coincides with cellular differentiation and the maturation of the PDL. Co-localization of periostin and collagen with subsequent fiber organization may help counteract tensional forces and reinforce the ECM structure. This may be a key mechanism of the PDL to adapt to occlusal forces and maintain structural integrity.

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Section: Discussionmentioning

confidence: 99%

Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function

et al. 2020

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“…Abnormal dentition was noted in one previous patient with a similarly large deletion of 8q21 (9.8 Mb; chr8: 74362438‐84172022), suggesting that a gene within 8q21 but outside the critical region for the syndrome may be involved in dentition. One gene within this region, STMN2 was recently identified to be upregulated in the periodontal ligament, the connector between the tooth and alveolar jaw bone, compared to the dental follicle [Lee et al, ]. Two other genes within the region involved in bone metabolism, IL7 and PAG1 , were also shown to be expressed in periodontal ligament cells with changes in expression in response to tensile strain [Pinkerton et al, ; Chang et al, ].…”

Section: Discussionmentioning

confidence: 99%

8q21.11 microdeletion in two patients with syndromic peters anomaly

Happ

Schilter

Weh

et al. 2016

American J of Med Genetics Pt A

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Peters anomaly is a form of anterior segment dysgenesis characterized by central ocular opacity and corneo-lenticular adhesions. Isolated and syndromic Peters anomaly can be observed and demonstrate significant genetic heterogeneity. We report the identification of overlapping 8q21.11 deletions in two patients with syndromic Peters anomaly via whole exome sequencing and chromosomal microarray analyses. Microdeletions of 8q21.11 were recently reported in ten patients with highly variable phenotypes involving craniofacial features, ptosis, intellectual disability, abnormalities of the hands/feet and other defects; sclerocornea and/or microphthalmia were reported in three cases. The two additional cases presented in this report expand the phenotypic spectrum of 8q21.11 microdeletions to include Peters anomaly (seen in both patients) and persistent primary dentition (seen in one patient with a larger deletion). The two novel deletions include the ZFHX4 and PEX2 genes, which are also affected in all three previous cases involving ocular anomalies. Screening of remaining alleles of the ZFHX4 and PEX2 did not identify any additional likely pathogenic variants in either patient, suggesting dominant (haploinsufficiency) mechanism for the identified deletion. This report provides further insight into the phenotypes associated with 8q21.11 deletions and, for the first time, reports Peters anomaly as an additional ocular feature; screening for copy number variations of the 8q21.11 region should be considered in patients with Peters anomaly and related syndromic features.

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“…This chemokine and its receptor may be involved in the progression of tissue destruction (including bone resorption) during periapical inflammation (45). A recent study found that the gene expression in the dental follicles was related to bone development and remodeling, apoptosis, and chemotaxis (15). This might also suggest that the immune responses of the APC are related to the development and remodeling of tissue.…”

Section: Discussionmentioning

confidence: 99%

“…The quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was performed using a modified version of a method used in previous studies (14)(15)(16). Diluted cDNA was used as a template for quantitative polymerase chain reaction (qPCR), which was performed using the ABI 7300 Real-Time PCR system (Applied Biosystems, Warrington, UK).…”

Section: Quantitative Reverse-transcription Polymerase Chain Reactionmentioning

confidence: 99%

Comparative Gene Expression Analysis of the Coronal Pulp and Apical Pulp Complex in Human Immature Teeth

Kim

Shin

et al. 2016

Journal of Endodontics

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Comparative Gene-Expression Analysis of the Dental Follicle and Periodontal Ligament in Humans

Cited by 33 publications

References 30 publications

Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function

Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function

8q21.11 microdeletion in two patients with syndromic peters anomaly

Comparative Gene Expression Analysis of the Coronal Pulp and Apical Pulp Complex in Human Immature Teeth

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