Comparative genome analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains isolated in Korea

Choi, Jong-Chul; Lee, Kun-Kyu; Pi, Jae Ho; Park, Seung‐Yong; Song, Chang‐Seon; Choi, In‐Soo; Lee, Joong‐Bok; Lee, Dong Hun

doi:10.1016/j.meegid.2014.06.005

Cited by 28 publications

(19 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The five US strains were reported to belong to North American clades I and II, following the PEDV outbreak in April 2013 (Vlasova et al., ). The three Korean strains are thought to be co‐circulating in Korea and showed high homology with the five US strains (Choi et al., ; Lee et al., ). The 31 isolated strains occupied all the 13 cities, suggesting that G3 was the most prevalent genotype in Shandong Province (Fig.…”

Section: Resultsmentioning

confidence: 99%

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Zhang

Tian

Lin

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

As one of the top pork producers in China, Shandong Province suffered frequent outbreaks of porcine epidemic diarrhoea virus (PEDV) on pig farms from January 2012 to July 2015, resulting in significant economic losses. To better understand the prevalence situation, we conducted molecular epidemiological analyses of 38 PEDV strains isolated from 13 cities in Shandong Province. The detection rate of PEDV was 71.2% (146/205) by reverse transcription polymerase chain reaction (RT-PCR). The S genes of the 38 isolated samples were 4146 to 4161 nt in length and shared high levels of sequence identity (93.3-99.6% nt, 92.1-99.4% aa) with those of the 41 reference strains. Among the 38 strains, 31 strains that occupied 12 cities were classed into G3 genotype, while the other seven that only existed in four cities were classed into G2 genotype. In addition, the strains CH-SDLY-2-2014 and CH-SDLY-3-2014 isolated from Linyi were classed into the Gd subgenotype. Notably, there were multiple insertions or deletions in the S genes and several mutations in the neutralizing epitopes of the PEDV S protein. Overall, the results revealed that G2 and G3 are the predominant PEDV genotypes circulating in Shandong Province during 2012-2015, and Gd subgenotype in G3 group had already spread towards northern China in 2014.

show abstract

Section: Resultsmentioning

confidence: 99%

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Zhang

Tian

Lin

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Overall, the virulence of highly virulent PEDV was characterized by disease/infection induced by a low infectious dose, short incubation times, severe clinical signs and intestinal lesions, and extensive PEDV replication throughout the intestine (Table 4). In addition to antigenic variation, these disease characteristics also explained why current highly virulent PED outbreaks were so devastating in the major pig producing countries in Asia (Choi et al, 2014;Oka et al, 2014;Sun et al, 2012) and North America (Stevenson et al, 2013;Vlasova et al, 2014). 4.3.…”

Section: Emerging Non-s Indel Pedv Strains Are Highly Virulent In Pigsmentioning

confidence: 99%

Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains

et al. 2016

View full text Add to dashboard Cite

Emerging and re-emerging coronaviruses cause morbidity and mortality in human and animal populations, resulting in serious public and animal health threats and economic losses. The ongoing outbreak of a highly contagious and deadly porcine epidemic diarrhea virus (PEDV) in Asia, the Americas and Europe is one example. Genomic sequence analyses of PEDV variants have revealed important insights into the evolution of PEDV. However, the antigenic variations among different PEDV strains are less explored, although they may contribute to the failure of PEDV vaccines in Asian countries. In addition, the evolution of PEDV results in variants with distinct genetic features and virulence differences; thus PEDV can serve as a model to explore the molecular mechanisms of coronavirus evolution and pathogenesis. In this article, we review the evolution, antigenic relationships and pathologic features of PEDV strains. This information and review of researches will aid in the development of strategies for control and prevention of PED.

show abstract

“…In 2014, several reports from Asian countries, including Korea, Taiwan and Japan [37,38], suggested that there has been an emergence of US-like PEDV in Asian countries. The results of our study, however, demonstrate that at present, PEDV in Vietnam is not influenced by US-like PEDV strains.…”

Section: Discussionmentioning

confidence: 99%

Complete genome characterization of porcine epidemic diarrhea virus in Vietnam

et al. 2015

View full text Add to dashboard Cite

Porcine epidemic diarrhea virus (PEDV) first emerged in Vietnam in 2009. In this study, the complete genomes of three Vietnamese PEDV isolates were characterized. These three isolates were isolated from 3-day-old pigs experiencing diarrhea. Two isolates were from swine farms in the south, and the other was from northern Vietnam. The whole genome sequences of these isolates are 28,035 nucleotides in length and have characteristics similar to those of other PEDV isolates. All three Vietnamese PEDV isolates share 99.8 % and 99.6 % sequence identity at the nucleotide and amino acid level, respectively, and have insertions of four amino acids (GENQ) and one amino acid (N) at positions 56-59 and 140, respectively, and one deletion of two amino acids (DG) at positions 160-161. Phylogenetic analysis based on the whole genome revealed that the three Vietnamese PEDV isolates are grouped together with new variants from China from 2011 to 2012 and are genetically distinct from US isolates and the classical PEDV variant. The results suggest that Vietnamese PEDV isolates are new variants, as evidenced by their genetic composition of insertions and a deletion in the spike gene, and they might have originated from the same ancestor as the Chinese PEDV strain. This study provides a better understanding of the molecular characteristics of PEDV in Vietnam.

show abstract

Comparative genome analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains isolated in Korea

Cited by 28 publications

References 13 publications

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Two Distinct Genotypes of Porcine Epidemic Diarrhoea Virus in Vaccinated Pig Flocks in Shandong Province of China, 2012-2015

Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains

Complete genome characterization of porcine epidemic diarrhea virus in Vietnam

Contact Info

Product

Resources

About