Comparative genomic analysis and identification of pathogenicity islands of hypervirulent ST-17 Streptococcus agalactiae Brazilian strain

Lannes-Costa, Pamella Silva; Baraúna, Rafael Azevedo; Ramos, Juliana Nunes; Veras, João Flávio Carneiro; Conceição, Marcos Vinícius Reis; Vieira, Verônica Viana; Mattos‐Guaraldi, Ana Luíza; Ramos, Rommel Thiago Jucá; Doran, Kelly S.; Silva, Artur M. S.; Nagao, Prescilla Emy

doi:10.1016/j.meegid.2020.104195

Cited by 9 publications

(4 citation statements)

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“…Notably, the main STs included in CC1 (ST1) and CC19 (ST19), in combination with different serotypes, have been associated with healthy carriers of Guillain-Barré syndrome and are responsible for the majority of GBS infections (Poyart et al, 2007;Bergal et al, 2015). The data obtained confirm that ST17, which is associated with capsular type III, has a known host-specific human profile, is associated with invasive neonatal diseases (Martins et al, 2010;Bergal et al, 2015;Lyhs et al, 2016;Lannes-Costa et al, 2020), and is one of the most frequent human STs, mainly in asymptomatic pregnant women. ST196 (serotype IV) was found in only three human isolates: one from a biopsy and two from vaginal swabs.…”

Section: Discussionmentioning

confidence: 52%

Evidence of Common Isolates of Streptococcus agalactiae in Bovines and Humans in Emilia Romagna Region (Northern Italy)

et al. 2021

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Streptococcus agalactiae (group B Streptococcus, GBS) is one of the most important agents of bovine mastitis and causes remarkable direct and indirect economic losses to the livestock sector. Moreover, this species can cause severe human diseases in susceptible individuals. To investigate the zoonotic potential of S. agalactiae, 203 sympatric isolates from both humans and cattle, isolated in the same time frame (2018) and in the same geographic area (Emilia Romagna region, Northern Italy), were characterized by molecular capsular typing (MCT), pilus island typing (PI), and multi-locus sequence typing (MLST). In addition, antibiotic-resistant phenotypes were investigated. The distribution of the allelic profiles obtained by combining the three genotyping methods (MCT-PI-MLST) resulted in 64 possible genotypes, with greater genetic variability among the human compared to the bovine isolates. Although the combined methods had a high discriminatory power (>96,2%), five genotypes were observed in both species (20,9% of the total isolates). Furthermore, some of these strains shared the same antibiotic resistance profiles. The finding of human and bovine isolates with common genotypes and antibiotic resistance profiles supports the hypothesis of interspecies transmission of S. agalactiae between bovines and humans.

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Section: Discussionmentioning

confidence: 52%

Evidence of Common Isolates of Streptococcus agalactiae in Bovines and Humans in Emilia Romagna Region (Northern Italy)

et al. 2021

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“…The Orthofinder software identified 1,473 core, 131 shared, and 127 singleton genes. In a study comparing the serotype III isolates only from human hosts, 1,610 core genes were predicted in the pan-genome ( Lannes-Costa et al, 2020 ). The closeness in the sizes of the core genomes observed in the aforementioned study and the present study reinforces the possibility of a strong genomic similarity among serotype III isolates.…”

Section: Resultsmentioning

confidence: 99%

In silico Prediction of New Drug Candidates Against the Multidrug-Resistant and Potentially Zoonotic Fish Pathogen Serotype III Streptococcus agalactiae

et al. 2020

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Streptococcus agalactiae is an invasive multi-host pathogen that causes invasive diseases mainly in newborns, elderly, and individuals with underlying health complications. In fish, S. agalactiae causes streptococcosis, which is characterized by septicemia and neurological signs, and leads to great economic losses to the fish farming industry worldwide. These bacteria can be classified into different serotypes based on capsular antigens, and into different sequence types (ST) based on multilocus sequence typing (MLST). In 2015, serotype III ST283 was identified to be associated with a foodborne invasive disease in non-pregnant immunocompetent humans in Singapore, and the infection was related to raw fish consumption. In addition, a serotype III strain isolated from tilapia in Brazil has been reported to be resistant to five antibiotic classes. This specific serotype can serve as a reservoir of resistance genes and pose a serious threat to public health. Thus, new approaches for the control and treatment of S. agalactiae infections are needed. In the present study, 24 S. agalactiae serotype III complete genomes, isolated from human and fish hosts, were compared. The core genome was identified, and, using bioinformatics tools and subtractive criteria, five proteins were identified as potential drug targets. Furthermore, 5,008 drug-like natural compounds were virtually screened against the identified targets. The ligands with the best binding properties are suggested for further in vitro and in vivo analysis.

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“…2020; Lannes‐Costa et al . 2020). The increasing stability and dominance of CC17 clone among neonatal invasive infections during the last decade indicate a high adaptability to this group of human patients.…”

Section: Pyogenic Groupmentioning

confidence: 99%

“…This review provides a synopsis of Streptococcus species related to human health, zoonotic Accepted Article specific gene sets that were not limited to particular geographical areas. The high prevalence of S. agalactiae ST17 strains among neonate infected patients and stillbirth cases has been described worldwide (Burcham et al 2019;Gori et al 2020;Lannes-Costa et al 2020). The increasing stability and dominance of CC17 clone among neonatal invasive infections during the last decade indicate a high adaptability to this group of human patients.…”

Section: Introductionmentioning

confidence: 98%

A current review of pathogenicity determinants of Streptococcus sp.

et al. 2021

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Comparative genomic analysis and identification of pathogenicity islands of hypervirulent ST-17 Streptococcus agalactiae Brazilian strain

Cited by 9 publications

References 75 publications

Evidence of Common Isolates of Streptococcus agalactiae in Bovines and Humans in Emilia Romagna Region (Northern Italy)

Evidence of Common Isolates of Streptococcus agalactiae in Bovines and Humans in Emilia Romagna Region (Northern Italy)

In silico Prediction of New Drug Candidates Against the Multidrug-Resistant and Potentially Zoonotic Fish Pathogen Serotype III Streptococcus agalactiae

A current review of pathogenicity determinants of Streptococcus sp.

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