Comparative genomic analysis of ovine and other host associated isolates of Staphylococcus aureus exhibit the important role of mobile genetic elements and virulence factors in host adaptation

Silva, Alessandra Lima da; Caetano, Ana Carolina Barbosa; Castillo, Raquel Hurtado; Santos, Roselane Gonçalves dos; Rodrigues, Diego Lucas Neres; Sousa, Thiago de Jesus; Kato, Rodrigo Bentes; Viana, Marcus Vinícius Canário; Gomide, Anne Cybelle Pinto; Aburjaile, Flávia Figueira; Tiwari, Sandeep; Jaiswal, Arun Kumar; Gala-García, Alfonso; Seyffert, Núbia; Castro, Thiago Luiz de Paula; Brenig, Bertram; Costa, Mateus Matiuzzi da; Dorneles, Elaine Maria Seles; Loir, Yves Le; Azevedo, Vasco

doi:10.1016/j.gene.2022.147131

Cited by 2 publications

(1 citation statement)

References 83 publications

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“…Together with some signals of cospeciation at higher taxonomic ranks in other mammals [i.e. Bacteroidaceae (Moeller et al, 2016), Bacteroidales (Groussin et al, 2017)], we reasoned that B. acidi-faciens represents an interesting model gut commensal to study bacterial genomic diversity according to host species, as comparable studies are largely limited to pathogenic taxa such as Burkholderia (Lee et al, 2021), Campylobacter (Costa et al, 2021), or Staphylococcus (Lima et al, 2022). Through a panel of genome-wide association analyses (GWAS) applied to pangenomic content, structural gene rearrangements, and SNPs, we reveal several candidates for adaptation to the host species environment, including genes involved in carbohydrate acquisition/utilization (SusD/RagB, amyA and amyS ) and de novo purine nucleotide biosynthesis ( purD ), which may serve as a basis for future studies of coadaptation within the mouse holobiont.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of novel Bacteroides acidifaciens isolates reveals candidates for adaptation to host subspecies in house mice

Fokt

Rühlemann

Godfroid

et al. 2023

Preprint

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The breadth of phenotypes influenced by the gut microbiome in multicellular hosts has attracted the keen and renewed interest of evolutionary biologists. Comparative studies suggest that coevolutionary processes may occur as hosts and their associated microbes (i.e., holobionts) diverge. The majority of studies to date however lack information beyond that of 16S rRNA gene profiling, and thus fail to capture potential underlying genomic changes among microbes. In this study, we conducted a comparative genomic analysis of 19 newly sampled Bacteroides acidifaciens isolates derived from the eastern and western house mouse subspecies, Mus musculus musculus and M. m. domesticus. Through a panel of genome-wide association (GWAS) analyses applied to pangenomic content, structural gene rearrangements, and SNPs, we reveal several candidates for adaptation to the host subspecies environment. The proportion of significant loci in each respective category is small, indicating low levels of differentiation according host subspecies. However, consistent signal is observed for genes involved in processes such as carbohydrate acquisition/utilization (SusD/RagB, amyA and amyS) and de novo purine nucleotide biosynthesis (purD), which serve as promising candidates for future experimental investigation in the house mouse as a model of holobiont evolution.

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Section: Introductionmentioning

confidence: 99%

Comparative genomics of novel Bacteroides acidifaciens isolates reveals candidates for adaptation to host subspecies in house mice

Fokt

Rühlemann

Godfroid

et al. 2023

Preprint

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Karakterisasi Feno-Genotipik Kemampuan Hemolisa Isolat Staphylococcus aureus Asal Susu Kambing Mastitis dan Daging Ayam Segar

Aziz,

Fitriana,

Setyorini

et al. 2023

J. Ilmu Peternak. dan Vet. Trop. (Journal Trop. Anim. Vet. Sci.

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Staphylococcus aureus (S. aureus) is a pathogenic bacterium with many virulence factors, such as toxins, immunomodulatory factors, and exoenzymes. Hemolysin is one of the essential toxins in the pathogenesis of S. aureus, with the action of lysing erythrocytes and other cells. This study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from animal origin. Eight S. aureus isolates from goat mastitis and raw chicken meat phenotypically evaluated the ability to hemolyze sheep blood. At the same time, genotypic were determinated by detecting the hla and hlb genes. The results showed that most 87.5% (7/8) of S. aureus isolates had β-hemolysis phenotypic. PCR determination showed that all (8/8) isolates were positive for the α-toxin (hla) encoding gene, while half (4/8) of the isolates were positive for the β-toxin (hlb) encoding gene. The use of 5% defibrinated sheep blood agar plates is recommended to determine the hemolytic character of S. aureus tested. Keywords: Character; Hemolysis; S. aureus Abstrak Staphylococcus aureus (S. aureus) merupakan bakteri patogen yang memiliki banyak faktor virulensi seperti toksin, faktor imunomodulator, dan eksoenzim. Hemolisin merupakan salah satu toksin penting dalam patogenesis S. aureus, dengan aksi kerja melisiskan eritrosit dan sel lainnya. Penelitian ini bertujuan untuk mengetahui karakter hemolisa isolat S. aureus asal hewan secara fenotipik dan genotipik. Delapan isolat S. aureus asal susu kambing mastitis dan daging ayam segar dievaluasi secara fenotipik untuk melihat kemampuan menghemolisa darah domba, sedangkan determinasi genotipik dengan deteksi gen hla dan gen hlb. Hasil penelitian menunjukkan mayoritas 87.5% (7/8) isolat S. aureus memiliki fenotipik β-hemolisa. Determinasi PCR menunjukkan 100% (8/8) isolat positif gen penyandi α-toksin (hla), sedangkan 50% (4/8) isolat positif gen penyandi β-toksin (hlb). Penggunaan 5% defibrinated pelat agar darah domba lebih direkomendasikan untuk melihat karakter hemolisa S. aureus yang diuji. Kata kunci: Hemolisa; Karakter; S. aureus

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Comparative genomic analysis of ovine and other host associated isolates of Staphylococcus aureus exhibit the important role of mobile genetic elements and virulence factors in host adaptation

Cited by 2 publications

References 83 publications

Comparative genomics of novel Bacteroides acidifaciens isolates reveals candidates for adaptation to host subspecies in house mice

Comparative genomics of novel Bacteroides acidifaciens isolates reveals candidates for adaptation to host subspecies in house mice

Karakterisasi Feno-Genotipik Kemampuan Hemolisa Isolat Staphylococcus aureus Asal Susu Kambing Mastitis dan Daging Ayam Segar

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